AUTHOR=Yang Yu-pei , Jian Yu-qing , Liu Yong-bei , Ismail Muhammad , Xie Qing-ling , Yu Huang-he , Wang Bin , Li Bin , Peng Cai-yun , Liu Bin , Man Rong-yong , Wang Wei 

TITLE=Triterpenoids From Kadsura coccinea With Their Anti-inflammatory and Inhibited Proliferation of Rheumatoid Arthritis-Fibroblastoid Synovial Cells Activities

JOURNAL=Frontiers in Chemistry

VOLUME=9

YEAR=2021

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2021.808870

DOI=10.3389/fchem.2021.808870

ISSN=2296-2646

ABSTRACT=<p>One new 3,4-<italic>seco</italic>-17,13-friedo-lanostane triterpenoid heilaohuacid A (<bold>1</bold>), one new 3,4-<italic>seco</italic>-17,14-friedo-lanostane triterpenoid heilaohuacid B (<bold>2</bold>), five new 3,4<italic>-seco</italic>-lanostane triterpenoids heilaohuacids C-D (<bold>3–4</bold>) and heilaohumethylesters A-C (<bold>7–9</bold>), one new 3,4-<italic>seco</italic>-cycloartane triterpenoid heilaohuacid E (<bold>5</bold>), and one new <italic>intact</italic>-lanostane triterpenoid heilaohuacid F (<bold>6</bold>), together with twenty-two known analogues (<bold>10–31</bold>), were isolated from heilaohu. Their structures were determined using HR-ESI-MS data, 1D and 2D NMR spectra, <sup>13</sup>C NMR calculations, and electronic circular dichroism (ECD) calculations. Heilaohuacids A and B (<bold>1</bold> and <bold>2</bold>) contain a 3,4-<italic>seco</italic> ring A and unprecedented migration of Me-18 from C-13 to C-17 or C-14 to C-18. This type of lanostane triterpenoid derivatives was rarely reported so far. More importantly, all compounds against inflammatory cytokines IL-6 and TNF-α levels on LPS-induced RAW 264.7 macrophages were evaluated, and compounds <bold>4</bold> and <bold>31</bold> significantly inhibited the release level of IL-6 with IC<sub>50</sub> values of 8.15 and 9.86 μM, respectively. Meanwhile, compounds 17, 18, and 31 significantly inhibited proliferation of rheumatoid arthritis-fibroblastoid synovial (RA-FLS) cells <italic>in vitro</italic> with IC<sub>50</sub> values of 7.52, 8.85, and 7.97 μM, respectively.</p>