AUTHOR=Wang Yan-Duo , Song Cheng-Gang , Yang Jian , Zhou Tao , Zhao Yu-Yang , Qin Jian-Chun , Guo Lan-Ping , Ding Gang 

TITLE=Accurate Identification of Degraded Products of Aflatoxin B1 Under UV Irradiation Based on UPLC-Q-TOF-MS/MS and NMR Analysis

JOURNAL=Frontiers in Chemistry

VOLUME=9

YEAR=2021

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2021.789249

DOI=10.3389/fchem.2021.789249

ISSN=2296-2646

ABSTRACT=<p>Analysis, purification, and characterization of AFB<sub>1</sub> degraded products are vital steps for elucidation of the photocatalytic mechanism. In this report, the UPLC-Q-TOF-MS/MS technique was first coupled with purification and NMR spectral approaches to analyze and characterize degraded products of AFB<sub>1</sub> photocatalyzed under UV irradiation. A total of seventeen degraded products were characterized based on the UPLC-Q-TOF-MS/MS analysis, in which seven ones (1–7) including four (stereo) isomers (1,2, 5, and 6) were purified and elucidated by NMR experiments. According to the structural features of AFB<sub>1</sub> and degraded products (1–7), the possible photocatalytic mechanisms were suggested. Furthermore, AFB<sub>1</sub> and degraded products (1–7) were evaluated against different cell lines. The results indicated that the UPLC-Q-TOF-MS/MS technique combined with purification, NMR spectral experiments, and biological tests was an applicable integrated approach for analysis, characterization, and toxic evaluation of degraded products of AFB<sub>1</sub>, which could be used to evaluate other mycotoxin degradation processes.</p>