AUTHOR=Luo Fang , Qin GeGe , Wang Lina , Fang Xiaohong TITLE=Single-Molecule Fluorescence Imaging Reveals GABAB Receptor Aggregation State Changes JOURNAL=Frontiers in Chemistry VOLUME=9 YEAR=2022 URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2021.779940 DOI=10.3389/fchem.2021.779940 ISSN=2296-2646 ABSTRACT=

The GABAB receptor is a typical G protein–coupled receptor, and its functional impairment is related to a variety of diseases. While the premise of GABAB receptor activation is the formation of heterodimers, the receptor also forms a tetramer on the cell membrane. Thus, it is important to study the effect of the GABAB receptor aggregation state on its activation and signaling. In this study, we have applied single-molecule photobleaching step counting and single-molecule tracking methods to investigate the formation and change of GABAB dimers and tetramers. A single-molecule stoichiometry assay of the wild-type and mutant receptors revealed the key sites on the interface of ligand-binding domains of the receptor for its dimerization. Moreover, we found that the receptor showed different aggregation behaviors at different conditions. Our results offered new evidence for a better understanding of the molecular basis for GABAB receptor aggregation and activation.