Xin Tian
Yu Chong
Cuicui Ge- State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, China
Due to the increasing amount of work being put into the development of nanotechnology, the field of nanomaterials holds great promise for revolutionizing biomedicine. However, insufficient understanding of nanomaterial-biological microenvironment (nano−bio) interactions hinders the clinical translation of nanomedicine. Therefore, a systematic understanding of nano−bio interaction is needed for the intelligent design of safe and effective nanomaterials for biomedical applications. In this review, we summarize the latest experimental and theoretical developments in the fields of nano−bio interfaces and corresponding biological outcomes from the perspective of corona and redox reactions. We also show that nano–bio interaction can offer a variety of multifunctional platforms with a broad range of applications in the field of biomedicine. The potential challenges and opportunities in the study of nano–bio interfaces are also provided.
Introduction
In recent years, the use of nanomaterials for targeted delivering and controlled releasing drugs, crossing biological barriers, activating immune cells, and reacting with redox species for diseases treatment (Zhang et al., 2018; Cai and Chen, 2019; Liu et al., 2019; Yang et al., 2019; Zhao et al., 2019) has been widely investigated. However, insufficient understanding of the interactions of nanomaterials with biological molecules and structures (such as, proteins, membranes, phospholipids, DNA, and free radicals) hinders the application of nanomedicine (Tian et al., 2016; Fang et al., 2017; Li et al., 2018; Yu et al., 2019). Upon entering into biological fluids, engineered nanomaterials can rapidly interact with various biomolecules, which mainly contain the three following aspects: (1) adsorption of biomolecules on the surface of nanomaterials, forming protein corona; (2) reconstruction and change of functional proteins; and (3) redox reactions between nanomaterials and reactive species (Scheme S1). These nano–bio interactions will not only greatly influence the function and fate of nanomaterials, but also affect cellular biological function (Liu et al., 2013). Therefore, it is important to evaluate the basic mechanisms of the reactions at nanomaterial–biology (nano–bio) interfaces and find strategies to manipulate the nano–bio reactions.
In this review, we reviewed current literature on the basic understanding of nano–bio interactions and their biological outcomes from associated nanotoxicity to promising biomedicine development including neurological disorders, bacterial infection, and cancer therapy. The knowledge presented here could lead to a better understanding of the nano–bio reactions and bring benefits to the development of nanomedicine.
Nanoparticle-Protein Interaction at the Nano-Bio Interface
Once entering the biological environment, engineered nanomaterials will immediately interact with surrounding biomolecules, especially the most abundant proteins, resulting in the formation of so-called protein corona, a term first coined by Dawson and co-workers in 2007 (Cedervall et al., 2007). Subsequently, we investigated the competitive binding of single-wall carbon nanotubes (SWCNTs) with highly abundant blood proteins [i.e., BSA, transferrin (Tf), gamma globulin (Ig), and bovine fibrinogen (BFG)] and found that hydrophobic interactions, especially π-π stacking interactions, are the driving forces behind the strong adsorption of serum proteins (Figure 1A) (Ge et al., 2011). Interestingly, two-dimensional graphene oxide (GO) nanosheets showed a much higher protein adsorption capacity than one-dimensional SWCNTs, although they exhibited similar binding model features (Figure 1B) (Chong et al., 2015). In addition, the nanoparticle-protein interaction underwent an intrinsically dynamic exchange at the nano-bio interface, forming “hard corona” containing higher affinity proteins or “soft corona” composed of lower affinity proteins (Chen et al., 2016).
Figure 1. Nanoparticle-protein interaction at the nano-bio interface. (A) AFM images and molecular modeling illustrations of SWCNTs coated by four abundant blood proteins (Ge et al., 2011) (with permission of National Academy of Sciences of the United States of America). (B) Quantitative analysis of serum proteins adsorption onto various carbon-based nanoparticles including GO, rGO, and SWCNTs (Chong et al., 2015) (with permission of American Chemical Society). (C) The interaction of nanoparticle–corona complex, rather than the bare nanoparticle, with biological machinery (Monopoli et al., 2012) (with permission of The Royal Society of Chemistry). (D) The recognition of protein coronas with diverse composition by different cell receptors, leading to the internalization via different mechanisms. (Francia et al., 2019) (with permission of Springer Nature). (E) Disruption of GO nanosheets on the actin cytoskeleton of A549 cells (Tian et al., 2017) (with permission of WILEY-VCH Verlag GmbH & Co. KGaA.). (F) AFM images of Aβ1-40 fibrils dissociated by GO treatment (Yang et al., 2015) (with permission of The Royal Society of Chemistry).
The presence of protein corona could affect the internalization and biodistribution of nanoparticles, even altering immune system activation and the final fate of nanomaterials (Figure 1C) (Monopoli et al., 2012). For instance, BSA corona greatly decreased the cellular uptake of GO by limiting its penetration into the cell membrane via the reduction of available surface area and lipid bilayer damaging (Duan et al., 2015). Corona's composition induced different endocytic pathways since the surface of nanoparticles was recognized by diverse cell receptors (Figure 1D) (Francia et al., 2019). When nanomaterials meet cell-conditioned media, such as, immune cells, it was found that the secretion of several specific cytokines could be either increased or mitigated owing to the formation of different protein corona on the surface of nanomaterials (Dai et al., 2017). In addition, upon the addition of graphene nanosheets, the structure of the intracellular cytoskeleton was dramatically disrupted (Tian et al., 2017), which retarded the cellular migration without causing acute cytotoxicity (Figure 1E). By exploiting this strong interaction between nanomaterials and proteins, carbon-based nanomaterials have been developed for to inhibit the fibrillation of amyloid-beta peptide or α-synuclein monomer, as well as effectively clearing the mature fibrils by destructively extracting peptide molecules from fibrils (Figure 1F) (Yang et al., 2015; Kim et al., 2019).
Redox Reaction at the Nano-Bio Interface
Redox reaction at the nano–bio interface is another critical factor that regulates the functions and toxicities of nanomaterials. Nanomaterials interact with these redox-related chemical species by generating and/or scavenging reactive oxygen species (ROS), which influences the fate of cells in vivo. Therefore, research on the interaction between nanomaterials and ROS not only help us to understand the mechanism of nanomaterial toxicity, but also broadens the applications of nanomaterials in medicine.
Numerous studies have demonstrated that many types of engineered nanomaterials are capable of accomplishing natural enzyme-like catalytic performance. For instance, iron oxide nanoparticles (Fe3O4) (Chen et al., 2012), graphene quantum dots (GQDs) (Sun et al., 2014), and Au nanoparticles (Wang et al., 2017) have oxidase- and/or peroxidase (POD)-like activities. In addition, we have investigated GO, GQDs, silver (Ag) nanoparticles, Pd nanoparticles, and Pd@Ir nanoparticles that have ROS-generating abilities (Chong et al., 2016, 2017; Ge et al., 2016; Fang et al., 2018; Cai et al., 2019; Tian et al., 2019). The ROS-generating abilities of these nanomaterials differ in sizes, shapes, and facets. For instance, Gao et al. have found that the POD-like activity of cooper nanoparticles is state-dependent (Figures 2A,B) (Xi et al., 2019). Furthermore, we have found that Pd nanoparticles with oxidase-like activity can catalyze the oxidation of ascorbate and generate H2O2. Pd nanoparticles enclosed by high-index facets remarkably amplify the oxidation of ascorbate, which is selective against cancer cells (Figures 2C–E) (Chong et al., 2018).
Figure 2. Redox reaction at the nano-bio interface. (A) TEM images of copper/carbon nanozymes. (B) Tuning catalytic activity by the copper state for antibacterial therapy. (C) TEM images of Pd nanocrystals. (Xi et al., 2019) (with permission of American Chemical Society). (D) Oxidation of ascorbate catalyzed by Pd nanocrystals. (E) Tumor growth curves from HCT116 tumor with ascorbate or Pd nanocrystals (Chong et al., 2018) (with permission of Springer Nature).
The scavenging of overexpressed ROS in pathological sites has been employed as a general therapeutic approach to pathological abnormalities, such as, Alzheimer's disease (Kwon et al., 2016), hepatitis (Zhang et al., 2016), and radiation damage (Cheng et al., 2018). Therefore, nanomaterials with catalase (CAT)- and/or superoxide dismutase (SOD)-like activities have been studied as therapeutic agents in ROS-related diseases. Metal-based nanomaterials, such as, cerium oxide (CeO2), manganese oxide (Mn3O4), Pd, and Pt, have attracted extensive attention due to their excellent enzyme-like activities (Chen et al., 2016; Kwon et al., 2016; Singh et al., 2017). For instance, custom-made CeO2 nanoparticles possessed SOD-like activity and can catalyze the reaction of to generate O2. These nanoparticles showed an excellent ability to protect neuronal cells from oxidative damage (Zeng et al., 2018). It is expected that these nanomaterials with a strong ability to scavenge ROS could be developed as a promising therapeutic agent for oxidative stress-related diseases.
Conclusions and Perspectives
Research on the nano–bio interfaces of engineered nanomaterials is an important issue in the development of nanomedicine. This is because nano–bio interfaces are related to the intelligent design of safe and effective nanomedicine, drug delivery, pathological site targeting, metabolism, and biocompatibility. In this review, we summarized recent advances in nano–bio interactions of nanomaterials from the perspective of corona and redox reactions. With these advances, the future use of nanomaterials in biomedicine will hold great promise, especially in ROS-related diseases. Nevertheless, the research of nano–bio interfaces still has many challenges: (1) A full understanding of the catalytic mechanisms of nanomaterials toward redox species is still lacking. (2) Regulation strategy on the catalytic activity of nanomaterials needs to be developed for their effective application as smarter therapeutic and diagnostic modalities. (3) Research on nano–bio interactions needs to consider the complex environment in vivo. (4) More attention should be paid to theoretical simulation in order to accurately and deeply investigate the nano–bio interactions. Thus, more efforts should be made in the research of nano–bio interactions.
Author Contributions
The manuscript was prepared by XT and YC, who contributed equally. CG conceived the review and contributed to the improvement of the manuscript.
Funding
This work was supported by the National Natural Science Foundation of China (Grand No. 31971320, 11575123, 21906110), the Natural Science Foundation of Jiangsu Province (BK20170353), a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection.
Conflict of Interest
The handling Editor declared a past co-authorship with one of the authors CG.
The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Supplementary Material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fchem.2020.00446/full#supplementary-material
References
Cai, R., and Chen, C. Y. (2019). The crown and the scepter: roles of the protein corona in nanomedicine. Adv. Mater. 31:e1805740. doi: 10.1002/adma.201805740
Cai, T., Fang, G., Tian, X., Yin, J. J., Chen, C., and Ge, C. (2019). Optimization of antibacterial efficacy of noble-metal-based core-shell nanostructures and effect of natural organic matter. ACS Nano 13, 12694–12702. doi: 10.1021/acsnano.9b04366
Cedervall, T., lynch, I., Lindman, S., Berggard, T, Thulin, E., Nilsson, H., Dawson, K. A., et al. (2007). Understanding the nanoparticle–protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles. Proc. Natl. Acad. Sci. U.S.A. 104, 2050–2055. doi: 10.1073/pnas.0608582104
Chen, F., Wang, G., Griffin, J., Brenneman, B., Banda, N., Holers, V., et al. (2016). Complement proteins bind to nanoparticle protein corona and undergo dynamic exchange in vivo. Nat. Nanotechnol. 12, 387–393. doi: 10.1038/nnano.2016.269
Chen, Z. W., Yin, J. J., Zhou, Y. T., Zhang, Y., Song, L., Song, M. J., et al. (2012). Dual enzyme-like activities of iron oxide nanoparticles and their implication for diminishing cytotoxicity. ACS Nano 6, 4001–4012. doi: 10.1021/nn300291r
Cheng, X., Ni, X., Wu, R., Chong, Y., Gao, X., Ge, C., et al. (2018). Evaluation of the structure-activity relationship of carbon nanomaterials as antioxidants. Nanomedicine 13, 733–747. doi: 10.2217/nnm-2017-0314
Chong, Y., Dai, X., Fang, G., Wu, R., Zhao, L., Ma, X., et al. (2018). Palladium concave nanocrystals with high-index facets accelerate ascorbate oxidation in cancer treatment. Nat. Commun. 9:4861. doi: 10.1038/s41467-018-07257-z
Chong, Y., Ge, C., Fang, G., Tian, X., Ma, X., Wen, T., et al. (2016). Crossover between anti- and pro-oxidant activities of graphene quantum dots in the absence or presence of light. ACS Nano 10, 8690–8699. doi: 10.1021/acsnano.6b04061
Chong, Y., Ge, C., Fang, G., Wu, R., Zhang, H., Chai, Z., et al. (2017). Light-enhanced antibacterial activity of graphene oxide, mainly via accelerated electron transfer. Environ. Sci. Technol. 51, 10154–10161. doi: 10.1021/acs.est.7b00663
Chong, Y., Ge, C., Yang, Z., Garate, J. A., Gu, Z., Weber, J., et al. (2015). Reduced cytotoxicity of graphene nanosheets mediated by blood-protein coating. ACS Nano 9, 5713–5724. doi: 10.1021/nn5066606
Dai, Q., Guo, J., Yan, Y., Ang, C. S., Bertleff-Zieschang, N., and Caruso, F. (2017). Cell-conditioned protein coronas on engineered particles influence immune responses. Biomacromolecules 18, 431–439. doi: 10.1021/acs.biomac.6b01545
Duan, G., Kang, S. G., Tian, X., Garate, J. A., Zhao, L., Ge, C., et al. (2015). Protein corona mitigates the cytotoxicity of graphene oxide by reducing its physical interaction with cell membrane. Nanoscale 7, 15214–15224. doi: 10.1039/C5NR01839K
Fang, G., Li, W., Shen, X., Perez-Aguilar, J. M., Chong, Y., Gao, X., et al. (2018). Differential Pd-nanocrystal facets demonstrate distinct antibacterial activity against Gram-positive and Gram-negative bacteria. Nat. Commun. 9:129. doi: 10.1038/s41467-017-02502-3
Fang, G., Luan, B. Q., Ge, C. C., Chong, Y., Dong, X., Guo, J., et al. (2017). Understanding the graphene quantum dots-ubiquitin interaction by identifying the interaction sites. Carbon 121, 285–291. doi: 10.1016/j.carbon.2017.05.096
Francia, V., Yang, K., Deville, S., Reker-Smit, C., Nelissen, I., and Salvati, A. (2019). Corona composition can affect the mechanisms cells use to internalize nanoparticles. ACS Nano 13, 11107–11121. doi: 10.1021/acsnano.9b03824
Ge, C., Du, J., Zhao, L., Wang, L., Liu, Y., Li, D., et al. (2011). Binding of blood proteins to carbon nanotubes reduces cytotoxicity. Proc. Natl. Acad. Sci. U.S.A. 108, 16968–16973. doi: 10.1073/pnas.1105270108
Ge, C., Fang, G., Shen, X., Chong, Y., Wamer, W. G., Gao, X., et al. (2016). Facet energy versus enzyme-like activities: the unexpected protection of palladium nanocrystals against oxidative damage. ACS Nano 10, 10436–10445. doi: 10.1021/acsnano.6b06297
Kim, D., Yoo, J., Hwang, H., Lee, J., Lee, S., Yun, S., et al. (2019). Graphene quantum dots prevent α-synucleinopathy in Parkinson's disease. Nat. Nanotechnol. 7, 779–786. doi: 10.1038/s41565-018-0179-y
Kwon, H. J., Cha, M. Y., Kim, D., Kim, D. K., Soh, M., Shin, K., et al. (2016). Mitochondria-targeting ceria nanoparticles as antioxidants for Alzheimer's disease. ACS Nano 10, 2860–2870. doi: 10.1021/acsnano.5b08045
Li, M., Gu, M. M., Tian, X., Xiao, B. B., Lu, S., Zhu, W., et al. (2018). Hydroxylated-graphene quantum dots induce DNA damage and disrupt microtubule structure in human esophageal epithelial cells. Toxicol. Sci. 164, 339–352. doi: 10.1093/toxsci/kfy090
Liu, J., Chen, C., and Zhao, Y. (2019). Progress and prospects of graphdiyne-based materials in biomedical applications. Adv. Mater. 31:e1804386. doi: 10.1002/adma.201804386
Liu, Y., Zhao, Y., Sun, B., and Chen, C. (2013). Understanding the toxicity of carbon nanotubes. ACC. Chem. Res. 46, 702–713. doi: 10.1021/ar300028m
Monopoli, M. P., Aberg, C., Salvati, A., and Dawson, K. A. (2012). Biomolecular coronas provide the biological identity of nanosized materials. Nat. Nanotechnol. 7, 779–786. doi: 10.1038/nnano.2012.207
Singh, N., Savanur, M. A., Srivastava, S., D'Silva, P., and Mugesh, G. (2017). A redox modulatory Mn3O4 nanozyme with multi-enzyme activity provides efficient cytoprotection to human cells in a Parkinson's disease model. Angew. Chem. Int. Edit. 56, 14267–14271. doi: 10.1002/anie.201708573
Sun, H. J., Gao, N., Dong, K., Ren, J. S., and Qu, X. G. (2014). Graphene quantum dots-band-aids used for wound disinfection. ACS Nano 8, 6202–6210. doi: 10.1021/nn501640q
Tian, X., Sun, Y., Fan, S., Boudreau, M. D., Chen, C., Ge, C., et al. (2019). Photogenerated charge carriers in molybdenum disulfide quantum dots with enhanced antibacterial activity. ACS Appl. Mater. Inter. 11, 4858–4866. doi: 10.1021/acsami.8b19958
Tian, X., Xiao, B. B., Wu, A., Yu, L., Zhou, J., Wang, Y., et al. (2016). Hydroxylated-graphene quantum dots induce cells senescence in both p53-dependent and -independent manner. Toxicol. Res. (Camb) 5, 1639–1648. doi: 10.1039/C6TX00209A
Tian, X., Yang, Z., Duan, G., Wu, A., Gu, Z., Zhang, L., et al. (2017). Graphene qxide nanosheets retard cellular migration via disruption of actin cytoskeleton. Small 13:1602133. doi: 10.1002/smll.201602133
Wang, Z. Z., Dong, K., Liu, Z., Zhang, Y., Chen, Z. W., Sun, H. J., et al. (2017). Activation of biologically relevant levels of reactive oxygen species by Au/g-C3N4 hybrid nanozyme for bacteria killing and wound disinfection. Biomaterials 113, 145–157. doi: 10.1016/j.biomaterials.2016.10.041
Xi, J., Wei, G., An, L., Xu, Z., Xu, Z., Fan, L., et al. (2019). Copper/carbon hybrid nanozyme: Tuning catalytic activity by the copper state for antibacterial therapy. Nano Lett. 19, 7645–7654. doi: 10.1021/acs.nanolett.9b02242
Yang, B., Chen, Y., and Shi, J. (2019). Nanocatalytic medicine. Adv. Mater. 31:1901778. doi: 10.1002/adma.201901778
Yang, Z., Ge, C., Liu, J., Chong, Y., Gu, Z., Jimenez-Cruz, C. A., et al. (2015). Destruction of amyloid fibrils by graphene through penetration and extraction of peptides. Nanoscale 7, 18725–18737. doi: 10.1039/C5NR01172H
Yu, L., Tian, X., Gao, D., Lang, Y., Zhang, X., Yang, C., et al. (2019). Oral administration of hydroxylated-graphene quantum dots induces intestinal injury accompanying the loss of intestinal stem cells and proliferative progenitor cells. Nanotoxicology 13, 1409–1421. doi: 10.1080/17435390.2019.1668068
Zeng, F., Wu, Y., Li, X., Ge, X., Guo, Q., Lou, X., et al. (2018). Custom-made ceria nanoparticles show a neuroprotective effect by nodulating phenotypic polarization of the microglia. Angew. Chem. Int. Edit.130, 5910–5914. doi: 10.1002/ange.201802309
Zhang, C., Wu, W., Li, R., Qiu, W. X., Zhuang, Z. N., Cheng, S., et al. (2018). Peptide-based multifunctional nanomaterials for tumor imaging and therapy. Adv. Funct. Mater. 28:1804492. doi: 10.1002/adfm.201804492
Zhang, W., Hu, S., Yin, J. J., He, W., Lu, W., Ma, M., et al. (2016). Prussian blue nanoparticles as multienzyme mimetics and reactive oxygen species scavengers. J. Am. Chem. Soc. 138, 5860–5865. doi: 10.1021/jacs.5b12070
Keywords: nanomaterials, nano–bio interface, corona, reactive oxygen species, nanomedicine
Citation: Tian X, Chong Y and Ge C (2020) Understanding the Nano–Bio Interactions and the Corresponding Biological Responses. Front. Chem. 8:446. doi: 10.3389/fchem.2020.00446
Received: 31 January 2020; Accepted: 28 April 2020;
Published: 10 June 2020.
Edited by:
Ruhong Zhou, IBM Research, United StatesReviewed by:
Bengt Fadeel, Karolinska Institutet (KI), SwedenFeng Ding, Clemson University, United States
Copyright © 2020 Tian, Chong and Ge. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Cuicui Ge, ccge@suda.edu.cn
†These authors have contributed equally to this work