AUTHOR=Zhao Ming , Qin Chunjun , Li Lingxin , Xie Haotian , Ma Beining , Zhou Ziru , Yin Jian , Hu Jing 

TITLE=Conjugation of Synthetic Trisaccharide of Staphylococcus aureus Type 8 Capsular Polysaccharide Elicits Antibodies Recognizing Intact Bacterium

JOURNAL=Frontiers in Chemistry

VOLUME=8

YEAR=2020

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2020.00258

DOI=10.3389/fchem.2020.00258

ISSN=2296-2646

ABSTRACT=<p><italic>Staphylococcus aureus</italic> causes a wide range of life-threatening diseases. One of the powerful approaches for prevention and treatment is to develop an efficient vaccine as antibiotic resistance greatly increases. <italic>S. aureus</italic> type 8 capsular polysaccharide (CP8) has shown great potential in vaccine development. An understanding of the immunogenicity of CP8 trisaccharide repeating unit is valuable for epitope-focused vaccine design and cost-efficient vaccine production. We report the chemical synthesis of conjugation-ready CP8 trisaccharide <bold>1</bold> bearing an amine linker, which effectively served for immunological evaluation. The trisaccharide <bold>1</bold>-CRM197 conjugate elicited a robust immunoglobulin G (IgG) immune response in mice. Both serum antibodies and prepared monoclonal antibodies recognized <italic>S. aureus</italic> strain, demonstrating that synthetic trisaccharide <bold>1</bold> can be an efficient antigen for vaccine development.</p>