AUTHOR=Lin Xiaona , Shi Yongli , Yu ShaSha , Li Siyi , Li Wenhui , Li Meishuang , Chen Shengxi , Wang Yuanbo , Cong Mei 

TITLE=Preparation of Poloxamer188-b-PCL and Study on in vitro Radioprotection Activity of Curcumin-Loaded Nanoparticles

JOURNAL=Frontiers in Chemistry

VOLUME=8

YEAR=2020

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2020.00212

DOI=10.3389/fchem.2020.00212

ISSN=2296-2646

ABSTRACT=<p>A novel polymer of poloxamer188-<italic>b</italic>-PCL was synthesized via a ring-opening polymerization. Fourier transform infrared spectroscopy (FTIR), Raman, and <sup>1</sup>H nuclear magnetic resonance (<sup>1</sup>H NMR) spectra were used to study the structures of obtained poloxamer188-<italic>b</italic>-PCL. The thermo-stability of poloxamer188 -<italic>b</italic>-PCL was carried out with a thermal gravimetric analyzer (TGA), and cytotoxicity was obtained using the CCK8 method. Cargo-free and curcumin (CUR)-loaded poloxamer188-<italic>b</italic>-PCL NPs were fabricated via the solvent evaporation method. The morphology, particle size distribution, and stability of cargo-free NPs were studied with a scanning electron microscope (SEM) and laser particle analyzer. The <italic>in vitro</italic> radioprotection activity of CUR-loaded NPs was performed. FTIR, Raman, and <sup>1</sup>H NMR spectra confirmed that poloxamer188-<italic>b</italic>-PCL was obtained. TGA curves suggested poloxamer188-<italic>b</italic>-PCL had better thermo-stability than original poloxamer188. Cell tests suggested that the cargo-free NPs had no cytotoxicity. SEM image showed that the cargo-free NPs were spherical with a diameter of 100 nm. Free radical scavenging experiments proved that CUR-loaded NPs had better antioxidant activity than CUR solutions. CUR-loaded NPs could be detected in all tissues, including liver, kidneys and lung. In summary, this work demonstrated a feasibility of developing an injective formulation of CUR and provided a protection agent in caner radiotherapy.</p>