AUTHOR=Goyard David , Thomas Baptiste , Gillon Emilie , Imberty Anne , Renaudet Olivier 

TITLE=Heteroglycoclusters With Dual Nanomolar Affinities for the Lectins LecA and LecB From Pseudomonas aeruginosa

JOURNAL=Frontiers in Chemistry

VOLUME=7

YEAR=2019

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2019.00666

DOI=10.3389/fchem.2019.00666

ISSN=2296-2646

ABSTRACT=<p>Multivalent structures displaying different instead of similar sugar units, namely heteroglycoclusters (hGCs), are stimulating the efforts of glycochemists for developing compounds with new biological properties. Here we report a four-step strategy to synthesize hexadecavalent hGCs displaying eight copies of αFuc and βGal. These compounds were tested for the binding to lectins LecA and LecB from <italic>Pseudomonas aeruginosa</italic>. While parent fucosylated (<bold>19</bold>) and galactosylated (<bold>20</bold>) homoclusters present nanomolar affinity with LecB and LecA, respectively, we observed that hGCs combining these sugars (<bold>11</bold> and <bold>13</bold>) maintain their binding potency with both lectins despite the presence of an unspecific sugar. The added multivalency is therefore not a barrier for efficient recognition by bacterial receptors and it opens the route for adding different sugars that can be selected for their immunomodulatory properties.</p>