AUTHOR=Gwynne Lauren , Sedgwick Adam C. , Gardiner Jordan E. , Williams George T. , Kim Gyoungmi , Lowe John P. , Maillard Jean-Yves , Jenkins A. Toby A. , Bull Steven D. , Sessler Jonathan L. , Yoon Juyoung , James Tony D. 

TITLE=Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells

JOURNAL=Frontiers in Chemistry

VOLUME=7

YEAR=2019

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2019.00255

DOI=10.3389/fchem.2019.00255

ISSN=2296-2646

ABSTRACT=<p>A long wavelength TCF-based fluorescent probe (<bold>TCF-ALP</bold>) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of <bold>TCF-ALP</bold> resulted in a significant fluorescence “turn on” (58-fold), which was accompanied by a colorimetric response from yellow to purple. <bold>TCF-ALP</bold> was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, <bold>TCF-ALP</bold> proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.</p>