AUTHOR=Liu Caiyan , Shi Chao , Li Mengzhe , Wang Mengyuan , Ma Cuiping , Wang Zonghua 

TITLE=Rapid and Simple Detection of Viable Foodborne Pathogen Staphylococcus aureus

JOURNAL=Frontiers in Chemistry

VOLUME=7

YEAR=2019

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2019.00124

DOI=10.3389/fchem.2019.00124

ISSN=2296-2646

ABSTRACT=<p><italic>Staphylococcus aureus</italic> (<italic>S. aureus</italic>) contamination in food safety has become a worldwide health problem. In this work, we utilized RNA one-step detection of denaturation bubble-mediated Strand Exchange Amplification (SEA) method to realize the detection of viable foodborne pathogen <italic>S. aureus</italic>. A pair of <italic>S. aureus</italic> specific primers were designed for the SEA reaction by targeting hypervariable V2 region of 16S rDNA and the amplification reaction was finished about 1 h. The results of amplification reaction could be observed by the naked eyes with a significant color change from light yellow to red to realize the colorimetric detection of <italic>S. aureus</italic>. Therefore, there only required an isothermal water bath, which was very popular for areas with limited resources. In real sample testing, although the SEA detection was so time-saving compared with the traditional plating method, the SEA method showed great consistency with the traditional plating method. In view of the above-described advantages, we provided a simple, rapid and equipment-free detection method, which had a great potential on ponit-of-care testing (POCT) application. Our method reported here will also provide a POCT detection platform for other food-borne pathogens in food, even pathogenic bacteria from other fields.</p>