AUTHOR=Obisesan Olaoluwa R. , Adekunle Abolanle S. , Oyekunle John A. O. , Sabu Thomas , Nkambule Thabo T. I. , Mamba Bhekie B. 

TITLE=Development of Electrochemical Nanosensor for the Detection of Malaria Parasite in Clinical Samples

JOURNAL=Frontiers in Chemistry

VOLUME=7

YEAR=2019

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2019.00089

DOI=10.3389/fchem.2019.00089

ISSN=2296-2646

ABSTRACT=<p>In this study, electrochemical nanosensors were developed from the synthesized metal oxide (MO) nanoparticles by supporting it on a gold electrode (Au). The activity of the developed nanosensor toward the detection of malaria biomarker (β-hematin) was determined and the optimum conditions at which the maximum detection and quantification occurred were established. β-Hematin current response at the sensors was higher when compared with the bare Au electrode and followed the order Au-CuO (C) &gt; Au-CuO (M) &gt; Au-Fe<sub>2</sub>O3 (M) &gt; Au-Fe<sub>2</sub>O3 (C) &gt; Au-Al<sub>2</sub>O3 (M) &gt; Au-Al<sub>2</sub>O3 (C) &gt; bare Au. The developed sensors were stable with a relatively low current drop (10.61–17.35 %) in the analyte. Au-CuO sensor had the best performance toward the biomarker and quantitatively detected <italic>P. berghei</italic> in infected mice's serum samples at 3.60–4.8 mM and <italic>P. falciparum</italic> in human blood serum samples at 0.65–1.35 mM concentration.</p>