AUTHOR=Cassiano Chiara , Esposito Roberta , Tosco Alessandra , Casapullo Agostino , Mozzicafreddo Matteo , Tringali Corrado , Riccio Raffaele , Monti Maria Chiara TITLE=Chemical Proteomics-Guided Identification of a Novel Biological Target of the Bioactive Neolignan Magnolol JOURNAL=Frontiers in Chemistry VOLUME=7 YEAR=2019 URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2019.00053 DOI=10.3389/fchem.2019.00053 ISSN=2296-2646 ABSTRACT=

Understanding the recognition process between bioactive natural products and their specific cellular receptors is of key importance in the drug discovery process. In this outline, some potential targets of Magnolol, a natural bioactive compound, have been identified by proteomic approaches. Among them, Importin-β1 has been considered as the most relevant one. A direct binding between Magnolol and this nuclear chaperone has been confirmed by DARTS and molecular docking, while its influence on Importin-β1 translocation has been evaluated by in vitro assays.