AUTHOR=Lindner Marco , Tresztenyak Aliz , Fülöp Gergö , Jahr Wiebke , Prinz Adrian , Prinz Iris , Danzl Johann G. , Schütz Gerhard J. , Sevcsik Eva TITLE=A Fast and Simple Contact Printing Approach to Generate 2D Protein Nanopatterns JOURNAL=Frontiers in Chemistry VOLUME=6 YEAR=2019 URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2018.00655 DOI=10.3389/fchem.2018.00655 ISSN=2296-2646 ABSTRACT=

Protein micropatterning has become an important tool for many biomedical applications as well as in academic research. Current techniques that allow to reduce the feature size of patterns below 1 μm are, however, often costly and require sophisticated equipment. We present here a straightforward and convenient method to generate highly condensed nanopatterns of proteins without the need for clean room facilities or expensive equipment. Our approach is based on nanocontact printing and allows for the fabrication of protein patterns with feature sizes of 80 nm and periodicities down to 140 nm. This was made possible by the use of the material X-poly(dimethylsiloxane) (X-PDMS) in a two-layer stamp layout for protein printing. In a proof of principle, different proteins at various scales were printed and the pattern quality was evaluated by atomic force microscopy (AFM) and super-resolution fluorescence microscopy.