AUTHOR=Yang Mingwang , Fan Jiangli , Du Jianjun , Long Saran , Wang Jia , Peng Xiaojun 

TITLE=Imaging of Formaldehyde in Live Cells and Daphnia magna via Aza-Cope Reaction Utilizing Fluorescence Probe With Large Stokes Shifts

JOURNAL=Frontiers in Chemistry

VOLUME=6

YEAR=2018

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2018.00488

DOI=10.3389/fchem.2018.00488

ISSN=2296-2646

ABSTRACT=<p>Formaldehyde (FA), a highly reactive carbonyl species, plays significant role in physiological and pathological functions. However, elevated FA will lead to cognitive impairments, memory loss and various neurodegenerative diseases due to its potent DNA and protein cross-linking mechanisms. In this work, a fluorescence probe, <bold>BD-CHO</bold>, based on benz-2-oxa-1, 3- diazole (<bold>BD</bold>) skeleton, was designed and synthesized for detection of FA via Aza-Cope reaction with high selectivity and large Stokes shifts (about 118 nm). <bold>BD-CHO</bold> was successfully applied to monitor the changes FA level in living cells, and kidney tissues of mice. Importantly it was the first time that <bold>BD-CHO</bold> was used for visualizing exogenous FA changes in <italic>Daphnia magna</italic> through fluorescence microscopy, demonstrating its potential application for studies of biological processes associated with FA.</p>