AUTHOR=Ayaz Muhammad , Junaid Muhammad , Ullah Farhat , Sadiq Abdul , Shahid Muhammad , Ahmad Waqar , Ullah Ihsan , Ahmad Ashfaq , Syed Nawazish-i-Husain 

TITLE=GC-MS Analysis and Gastroprotective Evaluations of Crude Extracts, Isolated Saponins, and Essential Oil from Polygonum hydropiper L.

JOURNAL=Frontiers in Chemistry

VOLUME=5

YEAR=2017

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2017.00058

DOI=10.3389/fchem.2017.00058

ISSN=2296-2646

ABSTRACT=<p>Peptic ulceration is among the most prevalent gastrointestinal disorders characterized by pepsin and gastric acid mediated mucosal damage, as result of imbalance between defensive and offensive processes. The main objective of the current study was to investigate the antiulcer potentials of <italic>Polygonum hydropiper</italic> crude methanolic ectract (Ph.Cr) in aspirin induced ulcerogenesis using pylorus ligated rat model. <italic>In-vitro</italic> urease and <italic>Proteus mirabilis</italic> inhibitory potentials were evaluated using standard protocols. All fractions were analyzed using GC-MS to identify major components. The aspirin induced ulcerogenesis in pylorus ligated rat model was associated with significant changes in the mean ulcer score [<italic>F</italic><sub>(5, 30)</sub> = 7.141, <italic>P</italic> = 0.0002], gastric juice volume [<italic>F</italic><sub>(5, 30)</sub> = 8.245, <italic>P</italic> &lt; 0.0001], gastric juice pH [<italic>F</italic><sub>(5, 30)</sub> = 5.715, <italic>P</italic> = 0.0008], free acidity [<italic>F</italic><sub>(5, 30)</sub> = 4.544, <italic>P</italic> = 0.0033], total acidity [<italic>F</italic><sub>(5, 30)</sub> = 2.740, <italic>P</italic> = 0.0373], and pepsin concentration [<italic>F</italic><sub>(5, 30)</sub> = 2.335, <italic>P</italic> = 0.0664]. Pre-treatment with Ph.Cr at 100, 200, and 400 mg/kg dose exhibited marked gastroprotective and anti-ulcerogenic effect in the aspirin induced pyloric ligation ulcerogenesis model at 100, 200, and 400 mg/kg as indicated by ulcerative biochemical parameters. In urease inhibition assay, leaves essential oil (Ph.Lo), saponins (Ph.Sp), and chloroform extract (Ph.Chf) exhibited highest activities with IC<sub>50</sub> of 90, 98, and 520 μg/ml, respectively. Ph.Sp, Ph.Chf, ethyl acetate (Ph.EtAc), and Ph.Cr showed MICs of 25, 30, 32.25, and 40.50 μg/ml, respectively against <italic>P. mirabilis</italic>. Several compounds were identified in GC-MS analysis of samples. Significant <italic>in-vivo</italic> antiulcer, urease inhibitory as well as anti-proteus potentials of <italic>P. hydropiper</italic> solvent extracts, signify its potential use for the management of peptic ulcers and may provide scientific bases for the traditional uses of the plant.</p>