AUTHOR=Spampinato Valentina , Parracino Maria Antonietta , La Spina Rita , Rossi Francois , Ceccone Giacomo TITLE=Surface Analysis of Gold Nanoparticles Functionalized with Thiol-Modified Glucose SAMs for Biosensor Applications JOURNAL=Frontiers in Chemistry VOLUME=4 YEAR=2016 URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2016.00008 DOI=10.3389/fchem.2016.00008 ISSN=2296-2646 ABSTRACT=

In this work, Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS), Principal Component Analysis (PCA) and X-ray Photoelectron Spectroscopy (XPS) have been used to characterize the surface chemistry of gold substrates before and after functionalization with thiol-modified glucose self-assembled monolayers and subsequent biochemical specific recognition of maltose binding protein (MBP). The results indicate that the surface functionalization is achieved both on flat and nanoparticles gold substrates thus showing the potential of the developed system as biodetection platform. Moreover, the method presented here has been found to be a sound and valid approach to characterize the surface chemistry of nanoparticles functionalized with large molecules. Both techniques were proved to be very useful tools for monitoring all the functionalization steps, including the investigation of the biological behavior of the glucose-modified particles in the presence of the maltose binding protein.