AUTHOR=Wong Alan , Boutin Céline , Aguiar Pedro M. 

TITLE=1H high resolution magic-angle coil spinning (HR-MACS) μNMR metabolic profiling of whole Saccharomyces cervisiae cells: a demonstrative study

JOURNAL=Frontiers in Chemistry

VOLUME=Volume 2 - 2014

YEAR=2014

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2014.00038

DOI=10.3389/fchem.2014.00038

ISSN=2296-2646

ABSTRACT=The low sensitivity of Nuclear Magnetic Resonance (NMR) is its prime shortcoming compared to other analytical methods for metabolomic studies. It relies on large sample volume (30–50 µl for HR-MAS) for rich metabolic profiling, hindering high-throughput screening especially when the sample requires a labor-intensive preparation or is a sacred specimen. This is indeed the case for some living organisms. This study evaluates a 1H HR-MAS approach for metabolic profiling of small volume (250 nl) whole bacterial cells, Saccharomyces cervisiae, using an emerging micro-NMR technology: high-resolution magic-angle coil spinning (HR-MACS). As a demonstrative study for whole cells, we perform two independent metabolomics studies identifying the significant metabolites associated with osmotic stress and aging.