AUTHOR=Li Qiang , Pan Ying , Han Li , Yang Yakun , Wu Xinran , Lei Yuguo TITLE=Improving three-dimensional human pluripotent cell culture efficiency via surface molecule coating JOURNAL=Frontiers in Chemical Engineering VOLUME=4 YEAR=2022 URL=https://www.frontiersin.org/journals/chemical-engineering/articles/10.3389/fceng.2022.1031395 DOI=10.3389/fceng.2022.1031395 ISSN=2673-2718 ABSTRACT=
Human pluripotent stem cells (hPSCs) are ideal “raw materials” for making various human cell types for regenerative medicine and are needed in large numbers. 3D suspension culturing (e.g., stirred-tank bioreactor or STR), which suspends and cultures cells in an agitated medium, has been extensively studied to scale up hPSC production. However, a significant problem with 3D suspension is the uncontrolled spheroid agglomeration. It leads to cell growth arrest, cell apoptosis, and inhomogeneity in cell purity and quality. We propose that i) inhibiting the spheroid adhesion can prevent spheroid agglomeration and ii) the inhibition can be achieved