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METHODS article

Front. Chem. Biol.
Sec. Structure, Spectroscopy & Imaging
Volume 3 - 2024 | doi: 10.3389/fchbi.2024.1425571
This article is part of the Research Topic Bioluminescence and Fluorescence Molecular Imaging in Chemical Biology View all 5 articles

Evaluation of the microenvironment formed by interferon-β

Provisionally accepted
Mai Yamagishi Mai Yamagishi 1,2*Yutaka Hori Yutaka Hori 3Nobutake Suzuki Nobutake Suzuki 1,2Yu Peng Yu Peng 4Yoshie Harada Yoshie Harada 4Takashi Funatsu Takashi Funatsu 2Osamu Ohara Osamu Ohara 5Yoshitaka Shirasaki Yoshitaka Shirasaki 2,6*
  • 1 Live Cell Diagnosis Co., Ltd, Saitama, Kanagawa, Japan
  • 2 Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo, Tōkyō, Japan
  • 3 Department of Applied Physics and Physico-informatics, Faculty of Science and Technology, Keio University, Hiyoshi Campus, Yokohama, Kanagawa, Japan
  • 4 Institute for Protein Research, Osaka University, Suita, Ōsaka, Japan
  • 5 Department of Applied Genomics, Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
  • 6 Research Center for Advanced Science and Technology, The University of Tokyo, Komaba, Japan

The final, formatted version of the article will be published soon.

    Heterogeneity in the cellular microenvironment in vivo affects the variability of reactivity among immune cells. Individual-specific microenvironmental differences play a crucial role in determining macroscopic outcomes, such as the efficacy of immunotherapy and disease progression. The microenvironment is also featured by cytokines released from cells, significantly regulating immune cell function. However, the overall understanding, at single-cell resolution, of how cytokines shape the microenvironment and promote paracrine signaling remains unclear. In this manuscript, we propose a methodology that addresses both the microenvironment itself and the response to the microenvironment to comprehend microenvironment behavior at the single-cell level. Our objective is to contribute to basic understanding of the interplay between immune cells and their microenvironment, with particular relevance to implications for immune therapy and disease progression.

    Keywords: cytokine, microenvironment, live-cell imaging, heterogeneity, immune response, IFN-β

    Received: 30 Apr 2024; Accepted: 18 Nov 2024.

    Copyright: © 2024 Yamagishi, Hori, Suzuki, Peng, Harada, Funatsu, Ohara and Shirasaki. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Mai Yamagishi, Live Cell Diagnosis Co., Ltd, Saitama, Kanagawa, Japan
    Yoshitaka Shirasaki, Research Center for Advanced Science and Technology, The University of Tokyo, Komaba, Japan

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.