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ORIGINAL RESEARCH article
Front. Cell. Neurosci.
Sec. Non-Neuronal Cells
Volume 19 - 2025 | doi: 10.3389/fncel.2025.1547905
This article is part of the Research Topic New Approaches To Overcome the Blood-Brain Barrier for the Treatment of CNS Disorders View all articles
The circulating CD4+, CD8+ and double negative T cells in ischemic stroke and stroke associated infection -a prospective case-control study
Provisionally accepted
Magdalena Telec 1*
Magdalena Frydrychowicz 1 Radosław Kazmierski 2 Izabela Wojtasz 3 Grzegorz Dworacki 1 Wojciech Kozubski 1
Maria Łukasik 1- 1 Poznan University of Medical Sciences, Poznań, Poland
- 2 University of Zielona Góra, Zielona Góra, Lubusz, Poland
- 3 Medicover, Poznan, Poland
Adaptive immunity after stroke results in the shift of T cells between the compartments, leading to peripheral lymphopenia and an increased number of T cells within the brain lesion.Stroke-associated infection (SAI) is a clinically significant issue in stroke units. The role of T cell subsets in a post-stroke immune response and SAI remains unclear. Thus we aimed to observe the quantitative changes of circulating CD4+, CD8+, double negative T cells, and CD4+/CD8+ ratio in stroke and SAI. We prospectively assessed the circulating CD4+, CD8+, and double negative T cells with flow cytometry in 52 patients on days 1.,3.,10. and 90. after ischemic stroke and we compared the results to those obtained in age-, sex-and vascular risk factor-matched controls. We analyzed lymphocyte parameters regarding clinical outcome, SAI, infarct lesion volume, and burden risk factors. There were no differences in studied parameters between stroke patients and controls as well as between subjects with SAI and those without infection. The higher percentage of CD4+ T cells and higher CD4+/CD8+ ratio correlated with better clinical status in the acute and subacute phase, while CD8+ T cells correlated oppositely. The percentage of CD8+ T cells correlated positively with CRP levels Sformatowano: Wyróżnienie in the acute and subacute phases of stroke, as well as in the control group. A negative correlation was noted between the percentage of CD4+ T cells on D1 and the serum level of CRP on D10 after stroke. Consistently, the CD4+/CD8+ ratio on D1 correlated negatively with CRP on D1, D3 and D10. In the patients with hypertension (HT) in history, an increased percentage of CD8+ T cells and a decreased percentage of CD4+ T cells as compared to those without HT, were found in the acute phase of stroke.
Keywords: ischemic stroke, stroke associated infection, CD4+ T cells, CD8+ T cells, CD4+/CD8+ratio, double negative T cells
Received: 18 Dec 2024; Accepted: 24 Feb 2025.
Copyright: © 2025 Telec, Frydrychowicz, Kazmierski, Wojtasz, Dworacki, Kozubski and Łukasik. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Magdalena Telec, Poznan University of Medical Sciences, Poznań, Poland
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