AUTHOR=Alam El Din Dowlette-Mary , Shin Jeongwon , Lysinger Alexandra , Roos Matthew J. , Johnson Erik C. , Shafer Timothy J. , Hartung Thomas , Smirnova Lena 

TITLE=Organoid intelligence for developmental neurotoxicity testing

JOURNAL=Frontiers in Cellular Neuroscience

VOLUME=18

YEAR=2024

URL=https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2024.1480845

DOI=10.3389/fncel.2024.1480845

ISSN=1662-5102

ABSTRACT=<p>The increasing prevalence of neurodevelopmental disorders has highlighted the need for improved testing methods to determine developmental neurotoxicity (DNT) hazard for thousands of chemicals. This paper proposes the integration of organoid intelligence (OI); leveraging brain organoids to study neuroplasticity <italic>in vitro,</italic> into the DNT testing paradigm. OI brings a new approach to measure the impacts of xenobiotics on plasticity mechanisms – a critical biological process that is not adequately covered in current DNT <italic>in vitro</italic> assays. Finally, the integration of artificial intelligence (AI) techniques will further facilitate the analysis of complex brain organoid data to study these plasticity mechanisms.</p>