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ORIGINAL RESEARCH article
Front. Cell. Neurosci.
Sec. Cellular Neurophysiology
Volume 18 - 2024 |
doi: 10.3389/fncel.2024.1455158
Magnetic field in the extreme low frequency band protects neuronal and microglia cells from oxygen-glucose deprivation
Provisionally accepted- 1 Achucarro Basque Center for Neuroscience, Leioa, Spain
- 2 Semi Zabala, DONOSTIA - SAN SEBASTIAN, Spain
- 3 Tecnalia Research & Innovation, San Sebastian, Spain
Ischemic stroke consists of rapid neural death as a consequence of brain vessel obstruction, followed by damage to the neighboring tissue known as ischemic penumbra. The cerebral tissue in the core of the lesions becomes irreversibly damaged, however, the ischemic penumbra is potentially recoverable during the initial phases after the stroke. Therefore, there is real need for emerging therapeutic strategies to reduce ischemic damage and its spread to the penumbral region. For this reason, we tested the effect of Extreme Low Frequency Electromagnetic Stimulation (ELF-EMS) on in vitro primary neuronal and microglial cultures under oxygen-glucose deprivation (OGD) conditions. ELF-EMS under basal non-OGD conditions did not induce any effect in cell survival. However, ELF-EMS significantly reduced neuronal cell death in OGD conditions and reduced ischemic induced Ca 2+ overload. Likewise, ELF-EMS modulated microglia activation and OGD-induced microglia cell death.Hence, this study suggests potential benefits in the application of ELF-EMS to limit ischemic irreversible damages under in vitro stroke conditions, encouraging in vivo preclinical validations of ELF-EMS as a potential therapeutic strategy for ischemic stroke.
Keywords: Stroke, Extreme Low Frequency Electromagnetic Stimulation (ELF-EMS), oxygen and glucose deprivation, Cell viability, Neuron, Microglia
Received: 26 Jun 2024; Accepted: 14 Oct 2024.
Copyright: © 2024 Mata, Calovi, Benli, Iglesias, Hernandez, Martin, Samartín, Ramos-Murguialday, Domercq and Ortego Isasa. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Iñaki Ortego Isasa, Tecnalia Research & Innovation, San Sebastian, 20009, Spain
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