Skip to main content

ORIGINAL RESEARCH article

Front. Cell. Neurosci.
Sec. Cellular Neurophysiology
Volume 18 - 2024 | doi: 10.3389/fncel.2024.1455158

Magnetic field in the extreme low frequency band protects neuronal and microglia cells from oxygen-glucose deprivation

Provisionally accepted
Gilda Paloma Mata Gilda Paloma Mata 1Stefano Calovi Stefano Calovi 1Kami Pars Benli Kami Pars Benli 2Leyre Iglesias Leyre Iglesias 1Maria Isabel Hernandez Maria Isabel Hernandez 1Abraham Martin Abraham Martin 1Alberto P. Samartín Alberto P. Samartín 1Ander Ramos-Murguialday Ander Ramos-Murguialday 3Maria Domercq Maria Domercq 1Iñaki Ortego Isasa Iñaki Ortego Isasa 3*
  • 1 Achucarro Basque Center for Neuroscience, Leioa, Spain
  • 2 Semi Zabala, DONOSTIA - SAN SEBASTIAN, Spain
  • 3 Tecnalia Research & Innovation, San Sebastian, Spain

The final, formatted version of the article will be published soon.

    Ischemic stroke consists of rapid neural death as a consequence of brain vessel obstruction, followed by damage to the neighboring tissue known as ischemic penumbra. The cerebral tissue in the core of the lesions becomes irreversibly damaged, however, the ischemic penumbra is potentially recoverable during the initial phases after the stroke. Therefore, there is real need for emerging therapeutic strategies to reduce ischemic damage and its spread to the penumbral region. For this reason, we tested the effect of Extreme Low Frequency Electromagnetic Stimulation (ELF-EMS) on in vitro primary neuronal and microglial cultures under oxygen-glucose deprivation (OGD) conditions. ELF-EMS under basal non-OGD conditions did not induce any effect in cell survival. However, ELF-EMS significantly reduced neuronal cell death in OGD conditions and reduced ischemic induced Ca 2+ overload. Likewise, ELF-EMS modulated microglia activation and OGD-induced microglia cell death.Hence, this study suggests potential benefits in the application of ELF-EMS to limit ischemic irreversible damages under in vitro stroke conditions, encouraging in vivo preclinical validations of ELF-EMS as a potential therapeutic strategy for ischemic stroke.

    Keywords: Stroke, Extreme Low Frequency Electromagnetic Stimulation (ELF-EMS), oxygen and glucose deprivation, Cell viability, Neuron, Microglia

    Received: 26 Jun 2024; Accepted: 14 Oct 2024.

    Copyright: © 2024 Mata, Calovi, Benli, Iglesias, Hernandez, Martin, Samartín, Ramos-Murguialday, Domercq and Ortego Isasa. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Iñaki Ortego Isasa, Tecnalia Research & Innovation, San Sebastian, 20009, Spain

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.