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ORIGINAL RESEARCH article

Front. Cell. Neurosci.
Sec. Non-Neuronal Cells
Volume 18 - 2024 | doi: 10.3389/fncel.2024.1442786

Anti-Neuroinflammatory Effects of Conjugated Linoleic Acid (CLA) Isomers, c9,t11 and t10,c12, on Activated BV-2 Microglial Cells

Provisionally accepted
Clara Porcedda Clara Porcedda Claudia Manca Claudia Manca Gianfranca Carta Gianfranca Carta Franca Piras Franca Piras Sebastiano Banni Sebastiano Banni Valeria Sogos Valeria Sogos *Elisabetta Murru Elisabetta Murru
  • Department of Biomedical Sciences, University of Cagliari, Cagliari, Sardinia, Italy

The final, formatted version of the article will be published soon.

    Conjugated linoleic acid (CLA) isomers exhibit anti-inflammatory properties within the central nervous system (CNS). This study investigated the effects of CLA isomers c9,t11 and t10,c12 on fatty acid (FA) and N-acylethanolamine (NAE) profiles and their association with pro-inflammatory molecule expression in BV-2 microglia cell line, the CNS's resident immune cells responsible for maintaining neuronal activity and immune homeostasis.BV-2 cells were treated with 25 µM of c9,t11-CLA, t10,c12-CLA, or oleic acid (OA) for 24 hours, followed by lipopolysaccharide (LPS) stimulation. After treatment, the cell's FA and NAE profiles and pro-inflammatory molecule expression were analyzed.Our results demonstrated that CLA isomers mitigate LPS-induced morphological changes in BV-2 cells and reduce gene expression and protein levels of inflammatory markers. This effect was linked to an upregulation of acyl-CoA oxidase 1, a key enzyme in the anti-inflammatory peroxisomal betaoxidation pathway that efficiently metabolizes CLA isomers. Notably, t10,c12-CLA significantly suppressed stearoyl-CoA desaturase 1, impacting monounsaturated fatty acid synthesis. The NAEs profile was remarkably altered by CLA isomers, with a significant release of the antineuroinflammatory mediator docosahexaenoic acid (DHA)-derived N-acylethanolamine (DHAEA).In conclusion, our findings suggest that the anti-neuroinflammatory effects of CLA isomers are due to their unique influences on FA metabolism and the modulation of bioactive FA-derived NAEs, highlighting a potential strategy for nutritional intervention in conditions characterized by neuroinflammation.

    Keywords: Conjugated linoleic acid (CLA), Microglia, Neuroinflammation, Nacylethanolamines (NAEs), inflammatory mediators

    Received: 02 Jun 2024; Accepted: 09 Sep 2024.

    Copyright: © 2024 Porcedda, Manca, Carta, Piras, Banni, Sogos and Murru. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Valeria Sogos, Department of Biomedical Sciences, University of Cagliari, Cagliari, Sardinia, Italy

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.