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ORIGINAL RESEARCH article

Front. Cell. Neurosci.
Sec. Non-Neuronal Cells
Volume 18 - 2024 | doi: 10.3389/fncel.2024.1421342
This article is part of the Research Topic Roles of Neuroinflammation and Remyelination in Neurodegenerative Disorders View all articles

Genome-wide sequencing identified extrachromosomal circular DNA as transcription factor-binding motif of the senescence genes that govern replicative senescence of human mesenchymal stem cells

Provisionally accepted
Wei Yang Wei Yang 1Wei Ji Wei Ji 2Boyu Liao Boyu Liao 3Zhongbo Li Zhongbo Li 1Jian Wang Jian Wang 1Haishu Lin Haishu Lin 3Jingbo Wang Jingbo Wang 3*Qian He Qian He 1
  • 1 Shenzhen Polytechnic University, shenzhen, China
  • 2 Changchun University of Science and Technology, changchun, China
  • 3 Shenzhen Technology University, Shenzhen, China

The final, formatted version of the article will be published soon.

    Mesenchymal stem cells (MSCs) have long been postulated as an important source cell in regenerative medicine. During subculture expansion, mesenchymal stem cell (MSC) senescence diminishes their multi-differentiation capabilities, leading to a loss of therapeutic potential. Up to date, the extrachromosomal circular DNAs (eccDNAs) have been demonstrated to be involved in senescence but the roles of eccDNAs during MSC senescence are yet unknown. Here we explored eccDNA profiles in human bone marrow MSCs (BM-MSCs), and gene annotation analysis revealed that the majority of eccDNA were mapped in the intron regions with limited BM-MSC enhancer overlaps.Sequentially, we discovered that these eccDNA motifs in senescent BMSCs acted as motifs for binding transcription factors (TFs) of senescence-related genes. These findings are highly significant for identifying biomarkers of senescence and therapeutic targets in mesenchymal stem cells (MSCs) for future clinical applications. The potential of eccDNA as a stable therapeutic target for senescence-related disorders warrants further investigation, particularly exploring chemically synthesized eccDNAs as transcription factor regulatory elements to reverse cellular senescence.

    Keywords: biomarker, Extrachromosomal circular DNAs, Mesenchymal Stem Cells, senescence, Transcription Factors

    Received: 22 Apr 2024; Accepted: 17 Jul 2024.

    Copyright: © 2024 Yang, Ji, Liao, Li, Wang, Lin, Wang and He. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Jingbo Wang, Shenzhen Technology University, Shenzhen, China

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