Qiang-qiang Xia
Anju Singh
Jing Wang1
Jeffrey D. Singer
Craig M. Powell- 1Department of Neurobiology, Marnix E. Heersink School of Medicine & Civitan International Research Center, University of Alabama at Birmingham, Birmingham, AL, United States
- 2Department of Biology, Portland State University, Portland, OR, United States
Autism Spectrum Disorders (ASDs) are neurodevelopmental disorders (NDDs) in which children display differences in social interaction/communication and repetitive stereotyped behaviors along with variable associated features. Cul3, a gene linked to ASD, encodes CUL3 (CULLIN-3), a protein that serves as a key component of a ubiquitin ligase complex with unclear function in neurons. Cul3 homozygous deletion in mice is embryonic lethal; thus, we examine the role of Cul3 deletion in early synapse development and neuronal morphology in hippocampal primary neuronal cultures. Homozygous deletion of Cul3 significantly decreased dendritic complexity and dendritic length, as well as axon formation. Synaptic spine density significantly increased, mainly in thin and stubby spines along with decreased average spine volume in Cul3 knockouts. Both heterozygous and homozygous knockout of Cul3 caused significant reductions in the density and colocalization of gephyrin/vGAT puncta, providing evidence of decreased inhibitory synapse number, while excitatory synaptic puncta vGulT1/PSD95 density remained unchanged. Based on previous studies implicating elevated caspase-3 after Cul3 deletion, we demonstrated increased caspase-3 in our neuronal cultures and decreased neuronal cell viability. We then examined the efficacy of the caspase-3 inhibitor Z-DEVD-FMK to rescue the decrease in neuronal cell viability, demonstrating reversal of the cell viability phenotype with caspase-3 inhibition. Studies have also implicated caspase-3 in neuronal morphological changes. We found that caspase-3 inhibition largely reversed the dendrite, axon, and spine morphological changes along with the inhibitory synaptic puncta changes. Overall, these data provide additional evidence that Cul3 regulates the formation or maintenance of cell morphology, GABAergic synaptic puncta, and neuronal viability in developing hippocampal neurons in culture.
Graphical Abstract.
Introduction
Autism spectrum disorders (ASDs) are common, sometimes debilitating disorders affecting social interaction/communication and repetitive behaviors (American Psychiatric Association, 2000; Lord et al., 2018). Approximately 15–20% of ASDs are caused by identifiable genetic mutations (Abrahams and Geschwind, 2010; Iossifov et al., 2014; Geschwind and State, 2015). Ubiquitin-proteasome degradation pathways are strongly implicated in ASD/NDD (Elgersma, 2015; Yi et al., 2015) with potential therapeutic approaches discovered (Huang et al., 2012). The Cul3 gene, coding for CUL3, a scaffolding component of an E3 ubiquitin ligase complex, is among several high-confidence ASD genes in exome sequencing studies focused on gene-deleterious, loss-of-function mutations (O'Roak et al., 2011, 2012a; De Rubeis et al., 2014; Iossifov et al., 2014; Geschwind and State, 2015; Sanders et al., 2015), making it of interest in understanding ASD pathophysiology.
Many susceptibility genes for autism are involved in regulating synapse formation, maintenance, or function (Kwon et al., 2006; Tabuchi et al., 2007; Blundell et al., 2010; Kouser et al., 2013; De Rubeis et al., 2014; Iossifov et al., 2014; Geschwind and State, 2015; Sanders et al., 2015; Araujo et al., 2017; Escamilla et al., 2017). Although little is known about the role of Cul3 in mammalian brain, deletion of the gene Kctd13, a gene in the 16p11.2 recurrent CNV region (Kumar et al., 2008; Marshall et al., 2008; Weiss et al., 2008; Bijlsma et al., 2009; McCarthy et al., 2009; Fernandez et al., 2010; Shinawi et al., 2010; Zufferey et al., 2012; Steinberg et al., 2014; D'Angelo et al., 2016) and a binding partner of CUL3, leads to decreased synaptic transmission and decreased synapse numbers in the hippocampus and other brain regions (Escamilla et al., 2017). Unlike Kctd13 homozygous deletion, however, deletion of Cul3 does not result in viable homozygous offspring (Singer et al., 1999) [wild-type (WT): 40.3%, heterozygous (Het): 59.7%, homozygous (Hom): 0 in 206 mice], indicating very different phenotypic effects in spite of some mechanistic overlap.
Previous studies indicate complex results of Cul3 heterozygous deletion in mouse brain morphology. Cul3 heterozygous deletion does not cause major neuronal morphology abnormalities except slightly decreased dendritic complexity (Rapanelli et al., 2019; Morandell et al., 2021; Xia et al., 2023), while another study reports decreased dendritic branching in primary cortical neurons (Amar et al., 2021). Cul3 heterozygous deletion decreases spine density in prefrontal cortical neurons (Rapanelli et al., 2019) but increases apical spine density in hippocampal CA1 pyramidal neurons (Dong et al., 2020). Loss of Cul3 also results in dysregulation of cytoskeletal proteins and impacts cytoskeletal dynamics (Amar et al., 2021; Morandell et al., 2021). These inconsistent results may be related to the different genetic backgrounds, models, or incompleteness/mosaicism of the deletion, which makes Cul3’s function in neurons, particularly during early development, unclear.
In addition, in-situ hybridization (ISH) data from the Allen Brain Atlas showed significantly high CUL3 expression in the pyramidal and granule cell layers of CA1 and DG of the hippocampus (Lin et al., 2023), and the hippocampus is important for several functions that are disrupted in ASD, e.g., social interaction (Hitti and Siegelbaum, 2014; Rubin et al., 2014; Tavares et al., 2015; Meira et al., 2018; Schafer and Schiller, 2018), spatial reasoning (Doeller et al., 2010; Jacobs et al., 2013; Lee et al., 2013; Brown et al., 2014) and memory (Fortin et al., 2002; Bartsch et al., 2011; Cooper et al., 2017; Cooper and Simons, 2019). Individuals with ASD may have abnormal hippocampal structure and function (Kemper and Bauman, 1993; Raymond et al., 1996; Bailey et al., 1998; Lawrence et al., 2010; Wegiel et al., 2010; Varghese et al., 2017). ASD symptoms typically become increasingly apparent when the hippocampus reaches major developmental milestones at around 2 years old (Banker et al., 2021). Thus, to understand how complete, homozygous deletion of Cul3 might affect early neuronal development, we created primary cultures of hippocampal neurons from postnatal day 1 (P1) brains and used adeno-associated virus (AAV) transduction to express either Cre-GFP (cre recombinase and green fluorescent protein) or GFP alone in WT, Het, and Hom fCul3 developing hippocampal neurons in vitro.
After confirming expected deletion of Cul3 in AAV-Cre-GFP-treated neurons, we identified axon, dendrite and spine morphological changes in Cul3 mutant neurons. Also, we examined the density and colocalization of pre- and post-synaptic excitatory/inhibitory synapse markers. Previous studies implicated elevated caspase-3 after Cul3 deletion (Kaplan et al., 2010; Amar et al., 2021; Morandell et al., 2021), and caspase-3 is known to modulate apoptosis (Wang and Lenardo, 2000; D'Amelio et al., 2012). Therefore we also confirmed elevated caspase-3 in our Cul3 deletion neurons and used caspase-3 inhibitor Z-DEVD-FMK (Shen et al., 2020; Deng et al., 2022) treatment to demonstrate whether Cul3 modulates neuronal viability, morphology, or synapse formation via caspase-3 activity in neurons.
Materials and methods
Animals
fCul3 mice (Figure 1A) were created in the Jeffrey D. Singer Lab at Portland State University (McEvoy et al., 2007) and were bred as heterozygous pairs to obtain WT, Het and Hom littermate pups. These fCul3 mice were originally made in a 129S1 genetic background (Jackson Labs Stock No: 002448) and then backcrossed to a C57BL/6 J background for at least 6 or more generations. All animal procedures were in accordance with the guidelines of the US National Institutes of Health Guidelines for the Care and Use of Laboratory Animals and approved by the Institutional Animal Care and Use Committee (IACUC) of UAB. Genotype of the floxed Cul3 gene was identified by PCR using four primers as follows:
WT (F): 5’-GAACTCAGTGCATGGTTGGA-3′;
WT (R): 5’-ACACCATGGACTATGAACATGC-3′;
Mutant (F): 5’-AGCCAACGCTATGTCCTGAT-3′;
Mutant (R): 5’-TCCTGCCGAGAAAGTATCCA-3′.
Figure 1. Cul3 knockout strategy and validation in primary cultured hippocampal neurons. (A) Schematic representation of fCul3 mouse model and viral transduction Cre-LoxP knockout strategy. Exons 3–8 of Cul3 are represented along with loxP, neomycin-resistance, and frt (flippase recognition target) sites. Transduction of neurons with AAV-Cre-GFP results in recombination and loss of Cul3 exons 4–7. AAV-GFP transduction is used as a negative control (not depicted). (B) Experimental timeline of neuronal culture and AAV transduction. fCul3 mouse pups are sacrificed at P1, cultures transduced with AAV viruses at DIV10, then examined for outcome measures at DIV16, 6 days after transduction (ICC, immunocytochemistry). (C,D) Representative immunoblot [left, (C)] and graph of group data [right, (D)] for anti-CUL3,anti-β-actin and anti-RhoA blots following either AAV-Cre-GFP (WT-Cre, Het-Cre, Hom-Cre) or AAV-GFP (WT-GFP, Het-GFP, Hom-GFP) transduction in cultured fCul3 hippocampal neurons. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ordinary two-way AVONA with Dunnett’s multiple comparisons test; bars represent mean ± SEM, 5 separately derived neuronal cultures.
WT primers produced a 382 bp band while mutant primers produced a 349 bp band.
Antibodies and reagents
Antibodies were used at the following dilutions from the following companies: rabbit anti-vGluT1 (1:500, SYSY, #135305), mouse anti-PSD95 (1:500, Millipore, #MAB1598), guinea pig anti-vGAT (1:500, SYSY, #131005), rabbit anti-gephyrin (1:500, SYSY, #147008), goat anti-GFP (1:1000, Novus Biological, #NB100-1770), mouse anti-Cul3 (1:500, BD Biosciences, #611848), mouse anti-RhoA (1:500, Abnova, #H00000387-M08), rabbit anti-cleaved caspase-3 (1:500, Cell Signaling, #9661), rabbit anti-p-mTOR (Ser2448) (1:500, Cell Signaling, #2971), rabbit anti-mTOR (1:500, Cell Signaling, #2983), rabbit anti-GluA1 (1:500, Millipore, #AB1504), rabbit anti-GRIN2A (1:500, Millipore, #07–632), rabbit anti-GABAAR α1 (1:500, Sigma, #G4416), mouse anti-β-actin (1:1000, MP Biomedicals, #691001). Secondary antibodies for immunocytochemistry were purchased from Jackson ImmunoResearch Laboratories (488 goat anti-rabbit, #111–545–003; Cy3 donkey anti-mouse, #715–165–150; Cy3 donkey anti-guinea pig, #706–165–148; 647 donkey anti-rabbit, #711–605–152) and Secondary antibodies for Western-Blot were purchased from LI-COR (goat anti-mouse-680, #926–68070 and goat anti-rabbit-800, #926–32211). Z-DEVD-FMK was purchased from R&D Systems (#FMK004) and added in cell cultures at a final concentration of 50 μM (Bieri et al., 2018; Alharris et al., 2019) in DMSO and Neurobasal A medium (Gibco, #10888022) supplemented with 10% horse serum (Gibco, #26050–070), which was considered as vehicle. Calcein AM was purchased from Invitrogen (#C34851) and added in cell cultures at a final concentration of 5 μM (Siveen et al., 2014; Huang et al., 2019).
AAV-virus production and transduction
AAV-Cre-GFP virus (AAV.CMV.HI.eGFP-Cre.WPRE.SV40, #105545-AAV2) and AAV-GFP virus (AAV.CMV.PI.EGFP.WPRE.bGH, #105530-AAV2) were purchased from Addgene. 1 μL virus at titer ≥7 × 1012 viral genomes/mL were used to transduce neurons cultured in each well of the 12-well plates at days in vitro (DIV) 10 followed by biochemical analyses or immunocytochemistry 6 days after transduction (Figure 1B).
Neuron culture and immunocytochemistry
Dissociated mouse primary hippocampal neurons (containing both excitatory and inhibitory neurons without glia) were cultured as previously described (Xu et al., 2010). Briefly, mouse hippocampus was dissected in HBSS Solution (Gibco, #14175095) at postnatal day 1 (P1) and digested into single cells by 2 mg/mL Papain (Worthington, #LS003127) for 20 min with 0.5 mg/mL of DNase I (Sigma Aldrich, #DN25) added during digestion. The neurons were plated on 12 mm coverslips coated with PDL (poly-D-lysine, Millipore Sigma, #A003E). Neurobasal A medium (Gibco, #10888022) supplemented with 10% horse serum (Gibco, #26050–070) and 1% Penicillin–Streptomycin (Invitrogen, #15140–142) were added, and coverslips were then incubated in a humidified CO2 (5%) incubator at 37° C. Medium was totally changed to Neuralbasal A medium supplemented with 2% B27 (Gibco, #17504–044), 1x GlutaMax (Invitrogen, #35050–061) and 1% Penicillin–Streptomycin after 1 h, and the medium was then half-changed 2 times per week. Cytarabine (Ara-C, Millipore, #147–94–4) was added at a concentration of 0.65 μM on DIV3 to inhibit glial cell proliferation.
For morphological experiments, neurons were fixed in 4% paraformaldehyde (Electron Microscopy Sciences, #15714) and 4% sucrose in Phosphate-buffered saline (PBS) for 15 min at room temperature (RT). Neuronal membranes were permeated with 0.2% triton X-100 in PBS (pH 7.3) for 10 min at RT. Then neurons were blocked in 10% Normal Donkey Serum (NDS, Jackson ImmunoResearch Laboratories, #017–000–121) in PBS for 2 h at RT (or 4°C overnight) and incubated with primary antibody at predetermined dilution in 3% NDS in PBS at 4°C overnight in a dark chamber. Samples were then washed 3 times with PBS after the cells were incubated with secondary antibodies for 1 h at RT. After 3 washes with PBS for 10 min each, neurons were mounted in Mowiol 4–88 mounting solution containing 6 g glycerol (Fisher Scientific, #G33), 2.4 g Mowiol 4–88 (Calbiochem, #475904), 6 mL ddH2O, 12 mL 0.2 mol/L Tris (Fisher Scientific, #BP152), and 2.5% DABCO (1,4-diazabicyclo[2.2.2]octane, Sigma Aldrich, #27802).
Immunoblotting
Crude lysates were collected in 2× Laemmli sample buffer (Bio-Rad, #1610737), supplemented with 2-Mercaptoethanol (Bio-Rad, #1610710), and boiled at 100°C for 5 min. Protein extracts were separated by SDS-PAGE and then transferred to a nitrocellulose membrane (Bio-Rad, #1704159). Membranes were blocked with Intercept Blocking Buffer (LI-COR, #927–60001) for 2 h at RT. Membranes were then incubated with the corresponding primary antibodies at 4°C overnight. The membranes were imaged on a LI-COR Odyssey CLx Imaging System after incubating with corresponding secondary antibodies. β-actin was used as an internal control with all protein levels normalized to the levels of these internal controls followed by normalizing all values to the average of the WT signals.
Microscopy, image processing, and analysis
Slides were visualized under a Zeiss LSM-800 Airyscan confocal microscope. Images were captured with the same parameters and analyzed blind to treatment/genotype. Control and experimental groups were processed in parallel. Branching of GFP-positive hippocampal neuron dendrites was measured using Neurolucida 360 software (MBF Bioscience). Spines on a single randomly selected secondary dendrite from each neuron were counted, spine density and spine size were measured by Neurolucida 360 software. Spines were classified into four subtypes as previous described (Harris et al., 1992; Hering and Sheng, 2001; Rochefort and Konnerth, 2012; Xia et al., 2023): thin, stubby, mushroom and filopodia. The classifying settings of Neurolucida 360 for spine subtypes were as follows: head to neck ratio = 1.1; length to head ratio = 2.5; mushroom head size = 0.35 μm; filopodium length = 3 μm. If the ratio of the total length of the protrusion to the width of base of the protrusion was smaller than 2.5, the spine was classified as stubby; if the ratio was larger than 2.5, but the head diameter < 0.35 μm, the spine was classified as thin. A mushroom spine was classified as a spine with a neck and a head diameter > 0.35 μm. A filopodia spine was classified as a long, thin spine without a clear head and a total length smaller than 3 μm (Xia et al., 2023). Sholl analysis was based on the number of intersections per radius (10 μm) of shell. Numbers of dendrites, total dendritic length and axon length were measured using confocal microscope images including all dendrites determined via Neurolucida 360. Number of dendrites was defined as all the primary, secondary, tertiary, etc. dendrites that were traced from cell body to the neurite terminal using Neurolucida 360 software. Both excitatory and inhibitory synaptic puncta were counted on every dendrite of each neuron using the same thresholds. The percentage of PSD95 overlapping vGluT1 and of gephyrin overlapping vGAT were performed by Neurolucida 360 software for colocalization analysis.
Statistics
Statistics were carried out using Graphpad Prism 10.2.2 (San Diego, CA, United States). Multiple comparisons were carried out using the ordinary two-way AVONA with Dunnett’s multiple comparisons test with genotype and treatment as the main variables. For Sholl analysis, spine density analysis, caspase-3 activity test and cell viability test, 30 neurons in total from 3 separately derived neuronal cultures were analyzed. For synaptic puncta density and colocalization analysis, 45 neurons in total from 3 separately derived neuronal cultures were analyzed. Blinded measurements were performed for all comparisons, see Supplementary Table S1 for detailed statistics. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, graphs depict mean ± SEM (Standard Error of Mean).
Results
Cul3 deletion from fCul3 mouse primary cultured hippocampal neurons
Because homozygous deletion of Cul3 in mice leads to embryonic lethality (Singer et al., 1999), we used the Cre-loxP system and cre recombinase expression to knockout Cul3 in hippocampal neurons cultured at P1 (Figures 1A,B). Following 10 days in vitro (DIV10) to establish the neurons in culture (Grabrucker et al., 2009), AAV-Cre-GFP or corresponding negative control AAV-GFP were applied to the cultures for 6 days (Figure 1B) during the period of peak synapse formation, ~DIV14 (Han and Stevens, 2009). Six days after AAV transduction (DIV16), we verified the efficiency of Cul3 deletion at the protein level by immunoblots (Figure 1C). AAV-Cre-GFP transduction effectively reduced CUL3 expression in Cul3 heterozygous hippocampal cultures to 52% of the AAV-Cre-GFP transduced WT control level and to 25% of the control level in Cul3 homozygous cultures (Figure 1D). RhoA is one of the downstream targets of CUL3, which is an essential regulator of neuronal growth and migration during early brain development, hence we also validated the RhoA expression (Lin et al., 2023). Well, no significant differences in RhoA levels were observed in AAV-Cre-GFP treated groups (Figure 1D). Thus, AAV-Cre-GFP transduction efficiently reduces CUL3 in fCul3 heterozygous neurons and virtually eliminates it in homozygous neurons.
Cul3 regulates axon and dendrite elaboration or maintenance and spine density in primary cultured hippocampal neurons
The effect of Cul3 deletion on dendritic arborization in Drosophila provides conflicting results (Zhu et al., 2005; Djagaeva and Doronkin, 2009), with one study revealing that dendritic elaboration is severely impaired in Cul3 mutants in Drosophila mushroom body neurons (Zhu et al., 2005) and another study showing the opposite result with loss of Cul3 causing excessive dendritic branching in Drosophila peripheral nervous system (Djagaeva and Doronkin, 2009). Also, previous studies of Cul3 heterozygous deletion in mice reach differing conclusions regarding morphological changes, some showing no effect (Rapanelli et al., 2019; Dong et al., 2020; Morandell et al., 2021; Xia et al., 2023), and others showing that Cul3 heterozygous deletion causes decreased dendritic arborization (Amar et al., 2021).
Because the function of Cul3 in dendritic morphology remains unclear and because loss of the BTB adaptor protein gene Kctd13 also alters dendritic arborization (Escamilla et al., 2017), we proceeded to elucidate the role of Cul3 in regulating neuronal morphology. First, we examined the effect of Cul3 deletion on dendritic arborization (Figure 2A), demonstrating that both heterozygous and homozygous loss of Cul3 reduced dendritic complexity as measured by the number of dendrite intersections at various distances from the soma (Sholl analysis, Figure 2B, right). No such differences were observed in control AAV-GFP-transduced WT, Het, or Hom fCul3 mutant neurons (Figure 2B, left). Total dendritic length was also significantly reduced in homozygous Cul3 deletion neurons (AAV-Cre-GFP treated) compared to control homozygous fCul3 neurons transduced with AAV-GFP (Figure 2C, middle). Significant decrease were also observed in the number of dendrites (Figure 2C, left). Axon length was significantly reduced in homozygous Cul3 deletion neurons (Figure 2C, right). Because mTOR (mammalian target of rapamycin) is involved in dendritic morphology (Kumar et al., 2005), we also performed immunoblots against p-mTOR and mTOR but interestingly found no p-mTOR/mTOR ratio differences among the AAV-Cre-treated groups (WT/Het/Hom, Supplementary Figure S1). These data suggest that Cul3 modulates dendrite arborization and length as well as axonal length or maintenance.
Figure 2. Cul3 deletion decreases dendrite elaboration or maintenance and increases dendritic spines of reduced volume in primary cultured hippocampal neurons. (A) Representative tracings (derived from Neurolucida 360 analysis of confocal images) of WT, Het, or Hom fCul3 cultured hippocampal neurons transduced with either AAV-GFP (left column) or AAV-Cre-GFP (right column). Red, cell soma; Black, dendrite; Green, axon. Scale bar, 50 μm. (B) Sholl analysis of WT-Cre, Het-Cre, and Hom-Cre Cul3 knockouts (right) demonstrates significantly decreased dendritic branching in both Het and Hom Cul3 mutant neurons. No change in dendritic branching is observed in control AAV-GFP transduced fCul3 neurons (left; WT-GFP, Het-GFP, Hom-GFP). (C) Number of dendrites (left), total dendritic length (middle) and axon length (right) significantly decreased in Cul3 homozygous knockout neurons (Hom-Cre) compared to control, fCul3 wildtype treated with AAV-Cre (WT-Cre). (D) Representative tracings (Neurolucida 360 analysis of confocal images) of WT, Het, and Hom fCul3 cultured hippocampal neuron dendritic spines transduced with AAV-GFP or AAV-Cre-GFP. White, thin; yellow, stubby; blue, mushroom; brown, filopodia. Scale bar, 20 μm. (E) Dendritic spine density significantly increased both in Het-Cre and Hom-Cre compared to control wildtype fCul3 neurons transduced with AAV-Cre (left). Average spine volume significantly reduced in homozygous Cul3 knockout neurons (Hom-Cre) compared to control homozygous fCul3 neurons transduced with AAV-GFP (middle). Density of various spine subtypes increased for thin and stubby spines only (right). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ordinary two-way AVONA with Dunnett’s multiple comparisons test; graphs depict mean ± SEM. N = 30 neurons from 3 separately derived neuronal cultures (10 neurons for each group).
The nature or presence of spine morphological changes after Cul3 heterozygous deletion in mice is also inconsistent among published studies. Decreased basal spine density (decreased thin spine density) in prefrontal cortical neurons was found in one study (Rapanelli et al., 2019); another study reports increased apical spine density and unchanged basal spine density in hippocampus CA1 pyramidal neurons (Dong et al., 2020), and other studies find unchanged spine density in different brain regions (Morandell et al., 2021; Xia et al., 2023). To determine effects on early spine development, we examined effects of Cul3 deletion on dendritic spines in cultured hippocampal neurons (Figure 2D). In contrast to the effects of Kctd13 deletion on dendritic spines (Escamilla et al., 2017), heterozygous and homozygous deletion of Cul3 by AAV-Cre-GFP in hippocampal neurons led to increased dendritic spine density (Figure 2E, left) but decreased average spine volume (Figure 2E, middle). We next examined which spine morphological subtypes (thin, stubby, mushroom, or filopodia) might be responsible for the increased spine density. We identified an increase in thin and stubby spines in Cul3 Het and Hom mutant neurons, while mushroom and filopodia spine numbers are comparable among all the groups (Figure 2E, right). These findings suggest that loss of Cul3 leads to increased formation or maintenance of dendritic spines that are on average smaller and possibly less mature, indicating a role for Cul3 in modulation of spine density and size.
Z-DEVD-FMK blocks Cul3 deletion-mediated caspase-3 activity in primary cultured hippocampal neurons
Previous studies revealed that Cul3 loss leads to a significant increase in caspase-3 activation which is thought to indicate subsequent neuronal cell death (Kaplan et al., 2010; Amar et al., 2021; Morandell et al., 2021). Previous studies also indicate that different caspase-3 activation level is a key regulatory mechanism of not only programmed cell death but also cell morphology (D'Amelio et al., 2010, 2012; Saric et al., 2022). Non-apoptotic caspase-3 functions like dendrite pruning or spine maturation also suggest that caspase-3 is a key regulatory molecule in neurogenesis and synapse formation/homeostasis (D'Amelio et al., 2010). In turn, we hypothesized that this pathway may also be a promising mechanism for altered neuronal morphology. So, we used Z-DEVD-FMK (Bieri et al., 2018; Alharris et al., 2019), a caspase-3 inhibitor peptide, to examine whether the Cul3 deletion phenotypes can be reversed by caspase-3 inhibition.
First, we examined caspase-3 activation in our Cul3 depleted neurons via immunohistochemistry and normalized caspase-3-activated neurons to all DAPI-stained neurons (Figure 3A). We validated the cell number based on DAPI-stained neurons and discovered significantly decreased cell numbers in Cul3 homozygous deletion group. This decreased cell density was reversed by caspase-3 activity inhibition (Figure 3B), and the transduction efficiencies were comparable among all the groups (Figure 3C). We confirmed that caspase-3 activation significantly increased in the Cul3 homozygous deletion group (Figure 3B), which was consistent with previous studies (Kaplan et al., 2010; Amar et al., 2021; Morandell et al., 2021). As a control, we also demonstrated that inhibiting caspase-3 prevented its activation (Figure 3D).
Figure 3. Cul3 modulates cell viability through caspase-3 activity in primary cultured hippocampal neurons. (A) Representative images of WT, heterozygous, and homozygous fCul3 cultured hippocampal neurons treated with Z-DEVD-FMK or vehicle transduced with AAV-Cre-GFP viruses (WT-Cre, Het-Cre, Hom-Cre). DAPI, cell nucleus marker. Cl. Caspase-3, cleaved caspase-3. Scale bar, 50 μm. (B) Neuron number counted based on DAPI signal. (C) AAV virus transduction efficiency was comparable among all groups. (D) Caspase-3 positive signal was divided by DAPI signal to determine the caspase-3-positive cell rate. Increased caspase-3 activity is largely reversed by Z-DEVD-FMK treatment in Hom group. (E) Representative images of WT, heterozygous, and homozygous fCul3 cultured hippocampal neurons treated with Z-DEVD-FMK or vehicle transduced with AAV-Cre-GFP viruses (WT-Cre, Het-Cre, Hom-Cre). Calcein, viable cell marker. Scale bar, 50 μm. (F) Neuron number based on DAPI signal. (G) AAV virus transduction efficiency was comparable among all groups. (H) Calcein positive signal was divided by DAPI signal to determine viable cell rate. Decreased viable cell rate is largely reversed by Z-DEVD-FMK treatment in both Het and Hom group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ordinary two-way AVONA with Dunnett’s multiple comparisons test; graphs depict mean ± SEM. N = 30 neurons from 3 separately derived neuronal cultures (10 neurons for each group).
Cul3 modulates cell viability through caspase-3 activity in primary cultured hippocampal neurons
Because caspase-3 activation can occur without neuronal death (Acarin et al., 2007; Erturk et al., 2014; Khatri et al., 2018; Saric et al., 2022), we examined whether the number of viable neurons were changed with Cul3 deletion. First, we confirmed that DAPI-stained cells were decreased in Cul3 deletion cultures (Figures 3E,F), and that this decrease was reversed by caspase-3 inhibition (Figures 3E,F). Transduction efficiency was again comparable (Figure 3G). Using a viable cell marker, Calcein AM (AM: Acetoxymethyl ester) normalized to DAPI-stained cells, we identified a decrease in viable neurons in the Cul3 Het and Hom deletion cultures that was also reversed by caspase-3 inhibition (Figure 3H). These data support the hypothesis that Cul3 modulates neuronal viability through caspase-3 activity in neurons. In addition, we observed a qualitative appearance of increased cell aggregation in the absence of Cul3 (Figures 3A,E), revealing potential mechanisms of cytoskeleton dysregulation in the absence of Cul3 in primary neuronal cultures, which are consistent with the previous findings (Amar et al., 2021; Morandell et al., 2021).
Cul3 regulates dendritic complexity & spines via caspase-3
Previous studies revealed that caspase-3 activity regulates not only cell death, but also cell morphology (Kaplan et al., 2010; Erturk et al., 2014; Guo et al., 2016; Hollville and Deshmukh, 2018; Amar et al., 2021; Morandell et al., 2021). Thus, we hypothesized that some of our neuronal morphology changes may result from elevated caspase-3 activity as well (Saric et al., 2022). To test this, we inhibited caspase-3 and examined neuronal morphology. Replicating our previous findings, Hom Cul3 mutant neurons demonstrated reduced dendrite and axon length as well as reduced dendrite complexity (Figures 4A–C), both Het and Hom Cul3 mutation increased spine density (Figures 4D,E), and Hom Cul3 mutant neurons demonstrated decreased spine volume (Figures 4D,E). The increased spine density mainly consists of thin and stubby spines (Figures 4D,E). Consistent with our hypothesis, caspase-3 inhibition largely reversed both dendrite, axon (Figures 4A–C) and spine (Figures 4D,E) morphological changes. These data indicate that Cul3 regulates dendritic complexity and spine generation at least in part via modulation of caspase-3 activity.
Figure 4. Cul3 regulates dendritic complexity and spine generation through caspase-3 activity in primary cultured hippocampal neurons. (A) Representative tracings (derived from Neurolucida 360 analysis of confocal images) of WT, heterozygous, and homozygous fCul3 cultured hippocampal neurons treated with Z-DEVD-FMK or vehicle transduced with AAV-Cre-GFP viruses (WT-Cre, Het-Cre, Hom-Cre). Red, cell soma; Black, dendrite; Green, axon. Scale bar, 50 μm. (B) Sholl analysis of WT-Cre, Het-Cre, and Hom-Cre Cul3 knockouts (left) demonstrates significantly decreased dendritic branching in both Het and Hom Cul3 mutant neurons. Decreased dendritic branching is largely reversed by Z-DEVD-FMK treatment in both Het and Hom group (right). (C) Number of dendrites (left), total dendritic length (middle) and axon length (right) significantly decreased in Cul3 homozygous knockout neurons (Hom-Cre) compared to control, fCul3 wildtype treated with AAV-Cre (WT-Cre). Decreased number of dendrites, total dendritic length and axon length were largely reversed by Z-DEVD-FMK treatment in Hom group. (D) Representative tracings (Neurolucida 360 analysis of confocal images) of WT, Het, and Hom fCul3 cultured hippocampal neuronal dendritic spines treated with Z-DEVD-FMK or vehicle transduced with AAV-Cre-GFP viruses (WT-Cre, Het-Cre, Hom-Cre). White, thin; yellow, stubby; blue, mushroom; brown, filopodia. Scale bar, 20 μm. (E) Dendritic spine density significantly increased in heterozygous and homozygous Cul3 knockout neurons were reversed by Z-DEVD-FMK treatment (upper left). Average spine volume significantly reduced in homozygous Cul3 knockout neurons was reversed by Z-DEVD-FMK treatment (upper right). Increased spine density with Cul3 deletion was largely due to increased thin and stubby spines increasing (lower left), and this was reversed by Z-DEVD-FMK (lower right). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ordinary two-way AVONA with Dunnett’s multiple comparisons test; graphs depict mean ± SEM. N = 30 neurons from 3 separately derived neuronal cultures (10 neurons for each group).
Cul3 deletion does not impact excitatory synaptic puncta formation in primary cultured hippocampal neurons
Because the combination of decreased total dendritic length and complexity, increased density of spines, and decreased volume of spines was difficult to interpret in terms of synapse number, we next examined whether Cul3 deletion impairs excitatory synaptic puncta formation. Thus, we labeled pre- and post-synaptic excitatory markers (vGluT1 and PSD95) (Figure 5A). Both vGulT1 (Figure 5B) and PSD95 (Figure 5C) puncta density was unchanged in both heterozygous and homozygous Cul3 deletion hippocampal neurons compared to heterozygous and homozygous fCul3 AAV-GFP transduced control neurons (Figures 5B,C). The percentage of co-localized PSD95/vGluT1 synaptic puncta compared to isolated pre- or post-synaptic puncta were comparable in homozygous Cul3 deletion neurons as well (Figure 5D). Because a previous report suggested decreased NMDAR-EPSC (excitatory postsynaptic currents) and unchanged AMPAR-EPSC input/output curves with a conditional heterozygous Cul3 deletion (Rapanelli et al., 2019), but we found comparable NMDA/AMPA current ratio between WT and Cul3 heterozygous neurons (Xia et al., 2023), so we performed immunoblots against NMDAR subunit GRIN2A and AMPAR subunit GluA1 and found no differences among the AAV-Cre-treated groups (WT/Het/Hom, Supplementary Figures S1A,C,D). These data suggest that Cul3 deletion does not significantly influence excitatory synaptic puncta density or colocalization in developing hippocampal neurons.
Figure 5. Cul3 homozygous deletion and heterozygous reduction lead to unchanged excitatory synapses in primary cultured hippocampal neurons. (A) Representative images of excitatory synaptic puncta in WT, heterozygous, and homozygous fCul3 cultured hippocampal neurons transduced with either AAV-GFP (WT-GFP, Het-GFP, Hom-GFP) or AAV-Cre-GFP (WT-Cre, Het-Cre, Hom-Cre). Scale bars represent 50 μm for the larger images and 20 μm for the magnified dendrite images. (B) Presynaptic excitatory synapse marker vGluT1 puncta density is comparable in both heterozygous and homozygous Cul3 knockout neurons (Het-Cre and Hom-Cre) compared to control heterozygous and homozygous fCul3 neurons transduced with AAV-GFP (Het-GFP and Hom-GFP). (C) Postsynaptic excitatory synapse marker PSD95 puncta density is unchanged in both heterozygous and homozygous Cul3 knockout neurons (Het-Cre and Hom-Cre) compared to control heterozygous and homozygous fCul3 neurons transduced with AAV-GFP. (D) Percentage of co-localized PSD95/vGluT1 puncta is unchanged in homozygous Cul3 knockout neurons (Hom-Cre) compared to homozygous fCul3 neurons transduced with AAV-GFP (Hom-GFP). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ordinary two-way AVONA with Dunnett’s multiple comparisons test; graphs depict mean ± SEM. N = 45 neurons from 3 separately derived neuronal cultures (15 neurons for each group).
Cul3 deletion impairs inhibitory synaptic puncta formation or maintenance
We next examined the density of inhibitory pre- and post-synaptic markers (vGAT and gephyrin) in Cul3 deletion hippocampal neurons (Figure 6A). In contrast to excitatory synaptic puncta, both inhibitory pre-synaptic marker puncta (Figure 6B) and inhibitory post-synaptic marker puncta (Figure 6C) were significantly reduced in both heterozygous and homozygous Cul3 deletion neurons (Figures 6B,C). The percentage of co-localized gephyrin/vGAT inhibitory synaptic puncta compared to isolated pre- or post-synaptic puncta was significantly reduced only in homozygous Cul3 deletion neurons (Figure 6D). We also measured GABAAR α1 subunits and found a significant reduction in protein expression with both Het and Hom Cul3 reduction/deletion (Supplementary Figures S1A,F). Taken together, these results suggest that Cul3 regulates the formation or maintenance of inhibitory synaptic puncta in early development of hippocampal neurons.
Figure 6. Cul3 homozygous deletion and heterozygous reduction lead to reduced inhibitory synapses in primary cultured hippocampal neurons. (A) Representative images of inhibitory synaptic puncta in WT, heterozygous, and homozygous fCul3 cultured hippocampal neurons transduced with either AAV-GFP (WT-GFP, Het-GFP, Hom-GFP) or AAV-Cre-GFP (WT-Cre, Het-Cre, Hom-Cre). Scale bars represent 50 μm for the larger images and 20 μm for the magnified dendrite images. (B) Presynaptic inhibitory synapse marker vGAT puncta density significantly decreased in both heterozygous and homozygous Cul3 knockout neurons (Het-Cre and Hom-Cre) compared to control heterozygous and homozygous fCul3 neurons transduced with AAV-GFP (Het-GFP and Hom-GFP). (C) Postsynaptic inhibitory synapse marker gephyrin puncta density significantly decreased in both heterozygous and homozygous Cul3 knockout neurons (Het-Cre and Hom-Cre) compared to control heterozygous and homozygous fCul3 neurons transduced with AAV-GFP (Het-GFP and Hom-GFP). (D) Percentage of co-localized gephyrin/vGAT puncta significantly decreased in homozygous Cul3 knockout neurons (Hom-Cre) compared to homozygous fCul3 neurons transduced with AAV-GFP (Hom-GFP). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ordinary two-way AVONA with Dunnett’s multiple comparisons test; graphs depict mean ± SEM. N = 45 neurons from 3 separately derived neuronal cultures (15 neurons for each group).
Cul3 regulates inhibitory synaptic puncta formation or maintenance via caspase-3
In addition to regulation of cell death and cell morphology, caspase-3 is also believed to modulate synaptic plasticity (Li et al., 2010; Wang et al., 2014; Gu et al., 2021; Fieblinger et al., 2022) and possibly inhibitory synapse elimination (Miller-Fleming et al., 2016). Thus, we hypothesized that the changes in inhibitory synaptic puncta number may result from elevated caspase-3 activity. To test this, we inhibited caspase-3 and examined inhibitory synaptic puncta. Replicating our previous findings, both Het and Hom Cul3 mutant vehicle-treated neurons demonstrated reduced pre- and post-synaptic inhibitory puncta density, and Hom Cul3 mutant vehicle-treated neurons revealed decreased percentage of co-localized gephyrin/vGAT synaptic puncta (Figures 7A–D). Interestingly, caspase-3 inhibition partially reversed both vGAT (Figures 7A,B) and gephyrin (Figures 7A,C) puncta density in both Het and Hom groups; caspase-3 inhibition also largely reversed the reduced gephyrin/vGAT inhibitory synaptic puncta co-localization in Hom Cul3 deletion neurons (Figure 7D). These findings are consistent with our hypothesis that Cul3 participates in regulating inhibitory synaptic puncta formation or maintenance in part via modulation of caspase-3 activity.
Figure 7. Caspase-3 inhibition partially rescues Cul3 deletion mediated reduced inhibitory synapses in primary cultured hippocampal neurons. (A) Representative images of inhibitory synaptic puncta in WT, heterozygous, and homozygous fCul3 cultured hippocampal neurons transduced with either Z-DEVD-FMK or vehicle transduced with AAV-Cre-GFP viruses (WT-Cre, Het-Cre, Hom-Cre). Scale bars represent 50 μm for the larger images and 20 μm for the magnified dendrite images. (B) Presynaptic inhibitory synapse marker vGAT puncta density significantly decreased in both heterozygous and homozygous Cul3 knockout neurons (Het-Cre and Hom-Cre) compared to control fCul3 neurons (WT-Cre). Decreased vGAT density is largely reversed by Z-DEVD-FMK treatment in both Het and Hom group. (C) Postsynaptic inhibitory synapse marker gephyrin puncta density significantly decreased in both heterozygous and homozygous Cul3 knockout neurons (Het-Cre and Hom-Cre) compared to control fCul3 neurons (WT-Cre). Decreased gephyrin density is largely reversed by Z-DEVD-FMK treatment in both Het and Hom group. (D) Percentage of co-localized gephyrin/vGAT puncta significantly decreased in homozygous Cul3 knockout neurons (Hom-Cre) which can be partially rescued by Z-DEVD-FMK. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ordinary two-way AVONA with Dunnett’s multiple comparisons test; graphs depict mean ± SEM. N = 45 neurons from 3 separately derived neuronal cultures (15 neurons for each group).
Discussion
Overall, our results indicate a role for Cul3 in modulation of dendritic length/complexity, axonal length, and dendritic spine and inhibitory synaptic puncta formation/maintenance in early mammalian hippocampal neuronal development. We have used developing neuronal cultures in vitro from an fCul3 model paired with AAV-Cre transduction due to the embryonic lethal nature of constitutive Cul3 homozygous deletion (Singer et al., 1999). For genes such as Cul3 for which neuronal function remains underexplored, homozygous deletion is among the best tools to elucidate function, particularly for autism risk genes (Blundell et al., 2009; Etherton et al., 2009; Zhou et al., 2009; Blundell et al., 2010; Espinosa et al., 2015; Escamilla et al., 2017; Jaramillo et al., 2017). Thus, our approach adds to the existing literature demonstrating effects of homozygous Cul3 deletion on neuronal function (Rapanelli et al., 2019; Dong et al., 2020; Fischer et al., 2020; Amar et al., 2021; Morandell et al., 2021).
Recent publications have examined Cul3 genetic mouse models (Rapanelli et al., 2019; Dong et al., 2020; Amar et al., 2021; Morandell et al., 2021), largely in the heterozygous state, and Cul3 neurons derived from human inducible pluripotent stem cells (Fischer et al., 2020). Contrary to our results in developing hippocampal neuronal cultures, a recent study on Emx1-Cre/fCul3 heterozygous mice showed that there was no change in number of intersections, number of dendrites and average dendritic length of prefrontal cortical pyramidal neurons much later in development in vivo (Rapanelli et al., 2019). Similarly, another study in pyramidal neurons of adult Cul3+/− mice somatosensory cortex also revealed normal dendrite morphology (Morandell et al., 2021). An in vitro study found a significant decrease of total dendrite length and number of dendrites in the Cul3+/− mice DIV14 cultured primary cortical neurons (Amar et al., 2021). None of these studies are directly comparable to ours due to the use of different brain regions and conditions.
Several lines of evidence in this study support a role for Cul3 in dendrite regulation in hippocampal neurons in culture. Homozygous loss of Cul3 leads to a decrease in total dendritic length and decreased dendritic branching by Sholl analysis. At the same time, synaptic spine density is increased, but spine volume is decreased in our cultured hippocampal neurons, and we have demonstrated that this spine density increase is largely mediated via immature appearing spines. Decreased dendritic length is expected to decrease dendritic area, potentially leading to decreased area for synaptic connections, yet spine density is increased, perhaps as a homeostatic compensation for decreased dendrites. It is possible that we are seeing the emergence of immature appearing spines that will later mature and make up for the loss of dendritic complexity. Actinfilin (a CUL3 substrate adaptor) is critical for enlargement and maintenance of dendritic spines and controls both the synaptic localization and the size of synaptic GluR6-containing kainate receptor clusters in hippocampal neurons, and actinfilin-CUL3-mediated degradation is important for regulating neuronal GluR6 surface expression (Salinas et al., 2006), which is strengthening the importance of Cul3’s role in actin cytoskeleton dynamics and a promising future direction for studying Cul3’s regulatory mechanism in cell morphology.
Our results demonstrate an increase in hippocampal neuron spine density in both heterozygous and homozygous Cul3 deletion; other studies focused mainly on heterozygous Cul3 deletion with varying results (Jaramillo et al., 2017; Rapanelli et al., 2019; Dong et al., 2020; Amar et al., 2021). The study on Emx1-Cre/fCul3 heterozygous mice showed a significant decrease in spine density of prefrontal cortical pyramidal neurons (Rapanelli et al., 2019). Another study on GFAP-Cre fCul3 heterozygous mice indicated significant increases in apical spine density and no change in basal spine density of hippocampal CA1 pyramidal neurons (Dong et al., 2020). A study in pyramidal neurons of adult Cul3+/− mice somatosensory cortex revealed unchanged spine density (Morandell et al., 2021). Again, it is difficult to compare these studies performed under different conditions and at different timepoints in development.
Previous studies revealed that Cul3 loss leads to a significant increase of caspase-3 activation (Kaplan et al., 2010; Amar et al., 2021; Morandell et al., 2021) and caspase-3 activation level is a key regulatory mechanism of cell morphology and programmed cell death (Erturk et al., 2014; Hollville and Deshmukh, 2018; Saric et al., 2022). By inhibiting caspase-3 activity, we discovered that the alteration in both dendrites and spines can be largely reversed to WT control levels.
On one hand, apoptotic stimuli such as DNA damage will lead to the release of cytochrome C (cyto-C) from mitochondria into the cytosol, combine with apoptotic protease activating factor-1 (Apaf-1) and then result in the activation of caspase-9; active caspase-9 further activates downstream effector caspases like caspase-3, caspase-6 and caspase-7 to initiate apoptosis (Wang and Lenardo, 2000; Schleich and Lavrik, 2013). On the other hand, death-receptor-induced caspase-8 activation can also activate downstream effector caspases and initiate apoptosis (Wang and Lenardo, 2000; Schleich and Lavrik, 2013). A previous study revealed that death receptor ligation induces polyubiquitination of caspase-8, through interaction of the death-inducing signaling complex (DISC) with a CUL3-based E3 ligase (Jin et al., 2009). The activation of caspase-3 leads to plasma membrane blebbing, chromatin condensation, DNA cleavage and phosphatidylserine exposure; it then produces the morphological and biochemical characteristics of apoptotic cells (Jiang et al., 2020). Our results indicates that Cul3 homozygous deletion significantly increases caspase-3 activity and subsequently increases cell apoptosis, which is agreeing with previous Cul3 studies (Amar et al., 2021; Morandell et al., 2021). Caspase-3 is also thought to mediate dendrite elaboration and dendritic complexity based on previous findings (Kaplan et al., 2010; Erturk et al., 2014; Guo et al., 2016; Hollville and Deshmukh, 2018; Amar et al., 2021; Morandell et al., 2021). Previous studies also demonstrated that inhibition of caspase-3 blocks spine shrinkage and leads to enlargement of spines (D'Amelio et al., 2011; Erturk et al., 2014). In agreement with these previous findings, our data indicate that Cul3 regulates dendritic complexity and spine generation or maintenance via caspase-3 activity.
It is becoming increasingly clear that caspase-3 regulates synaptic plasticity and contributes to learning and memory as a non-apoptotic function (Li et al., 2010; Wang et al., 2014; Gu et al., 2021; Fieblinger et al., 2022). Staining for excitatory pre- and post-synaptic markers demonstrates unchanged excitatory synaptic puncta density and co-localization in our Cul3 homozygous deletion cultured neurons. A previous study also indicated that UNC-8 (UNC, un-coordinated) triggers a caspase-dependent mechanism to drive GABA neuron synapse elimination (Miller-Fleming et al., 2016). It is interesting that a significant decrease in inhibitory synaptic puncta occurs in heterozygous Cul3 deletion neurons; heterozygous Cul3 loss best mimics the genotype of ASD patients with Cul3 loss-of-function mutations (Kong et al., 2012; O'Roak et al., 2012a,b; De Rubeis et al., 2014; Iossifov et al., 2014; Deciphering Developmental Disorders S, 2015; Sanders et al., 2015; da Silva Montenegro et al., 2020; Nakashima et al., 2020). Similarly, homozygous loss of Cul3 also leads to a significantly decreased fraction of inhibitory pre/post-synaptic marker co-localization. Interestingly, we demonstrate that caspase-3 inhibition partially rescues the decreased inhibitory synaptic puncta in heterozygous and homozygous Cul3 deletion neurons, revealing a potential role for Cul3 in regulating inhibitory synapses via caspase-3 activity.
Overall, our findings demonstrate that Cul3 regulates axon and dendrite elaboration or maintenance, neuronal viability, spine density and inhibitory synaptic puncta density via caspase-3 activity in primary cultured hippocampal neurons, furthermore, these alterations in early neuronal development can largely be rescued by caspase-3 inhibition. In conclusion, Cul3 regulates the formation or maintenance of cell morphology, GABAergic synaptic puncta, and neuronal viability in developing hippocampal neurons in culture.
Data availability statement
The dataset analyzed in this paper is available at the GIN repository (https://doi.org/10.12751/g-node.qdqcp3).
Ethics statement
The animal study was approved by Institutional Animal Care and Use Committee (IACUC) of University of Alabama at Birmingham. The study was conducted in accordance with the local legislation and institutional requirements.
Author contributions
Q-qX: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Validation, Visualization, Writing – original draft, Writing – review & editing. AS: Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – review & editing. JW: Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – review & editing. ZX: Methodology, Resources, Validation, Writing – review & editing. JS: Resources, Writing – review & editing, Methodology. CP: Conceptualization, Funding acquisition, Methodology, Project administration, Resources, Supervision, Writing – review & editing.
Funding
We acknowledge funding from the National Institute of Mental Health (NIMH) R01 MH113948 (to CP) and Civitan International Research Center Emerging Scholar Award (to Q-qX). We appreciate use of UAB Civitan International Research Center Cellular Neuroimaging Core. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Acknowledgments
We would like to thank Jeffrey D. Singer Lab at Portland State University for sharing the floxed Cul3 (fCul3) mice. We also would like to thank all members of the Powell Laboratory for critical discussions, sharing reagents, and providing technical assistance.
Conflict of interest
CP has been funded in the past by Novartis and reimbursed for travel to speak once each at Dainippon Sumitomo Pharma Co., Pfizer, Roche, Astra-Zeneca, and Psychogenics. CP has also served once as a paid expert witness for attorneys representing Johnson & Johnson, Inc., among other defendants.
The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Supplementary material
The Supplementary material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fncel.2024.1320784/full#supplementary-material
SUPPLEMENTARY FIGURE S1 | Cul3 homozygous deletion and heterozygous reduction decrease GABAA receptor but not ionotropic glutamate receptor expression in primary cultured hippocampal neurons. (A–F) Representative immunoblots (A) and graph of group data (B–F) for anti-p-mTOR, anti-mTOR, anti-GRIN2A, anti-GluA1, anti-CUL3, anti-GABAAR α1 and anti-β-actin blots following AAV-Cre-GFP (WT-Cre, Het-Cre, Hom-Cre) transduction in cultured fCul3 hippocampal neurons. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ordinary one-way AVONA with Dunnett’s multiple comparisons test; bars represent mean ± SEM, 4 separately derived neuronal cultures.
SUPPLEMENTARY TABLE S1 | Detailed statistical analysis for all comparisons.
References
Abrahams, B. S., and Geschwind, D. H. (2010). Connecting genes to brain in the autism spectrum disorders. Arch. Neurol. 67, 395–399. doi: 10.1001/archneurol.2010.47
Acarin, L., Villapol, S., Faiz, M., Rohn, T. T., Castellano, B., and Gonzalez, B. (2007). Caspase-3 activation in astrocytes following postnatal excitotoxic damage correlates with cytoskeletal remodeling but not with cell death or proliferation. Glia 55, 954–965. doi: 10.1002/glia.20518
Alharris, E., Singh, N. P., Nagarkatti, P. S., and Nagarkatti, M. (2019). Role of mi RNA in the regulation of cannabidiol-mediated apoptosis in neuroblastoma cells. Oncotarget 10, 45–59. doi: 10.18632/oncotarget.26534
Amar, M., Pramod, A. B., Yu, N. K., Herrera, V. M., Qiu, L. R., Moran-Losada, P., et al. (2021). Autism-linked Cullin 3 germline haploinsufficiency impacts cytoskeletal dynamics and cortical neurogenesis through rho a signaling. Mol. Psychiatry 26, 3586–3613. doi: 10.1038/s41380-021-01052-x
American Psychiatric Association . Task force on DSM-IV. diagnostic and statistical manual of mental disorders: DSM-IV-TR. 4th Washington, DC: American Psychiatric Association; (2000).
Araujo, D. J., Toriumi, K., Escamilla, C. O., Kulkarni, A., Anderson, A. G., Harper, M., et al. (2017). Foxp 1 in forebrain pyramidal neurons controls gene expression required for spatial learning and synaptic plasticity. J. Neurosci. 37, 10917–10931. doi: 10.1523/JNEUROSCI.1005-17.2017
Bailey, A., Luthert, P., Dean, A., Harding, B., Janota, I., Montgomery, M., et al. (1998). A clinicopathological study of autism. Brain 121, 889–905. doi: 10.1093/brain/121.5.889
Banker, S. M., Gu, X., Schiller, D., and Foss-Feig, J. H. (2021). Hippocampal contributions to social and cognitive deficits in autism spectrum disorder. Trends Neurosci. 44, 793–807. doi: 10.1016/j.tins.2021.08.005
Bartsch, T., Dohring, J., Rohr, A., Jansen, O., and Deuschl, G. (2011). CA1 neurons in the human hippocampus are critical for autobiographical memory, mental time travel, and autonoetic consciousness. Proc. Natl. Acad. Sci. USA 108, 17562–17567. doi: 10.1073/pnas.1110266108
Bieri, G., Lucin, K. M., O'Brien, C. E., Zhang, H., Villeda, S. A., and Wyss-Coray, T. (2018). Proteolytic cleavage of Beclin 1 exacerbates neurodegeneration. Mol. Neurodegener. 13:68. doi: 10.1186/s13024-018-0302-4
Bijlsma, E. K., Gijsbers, A. C., Schuurs-Hoeijmakers, J. H., van Haeringen, A., Fransen van de Putte, D. E., Anderlid, B. M., et al. (2009). Extending the phenotype of recurrent rearrangements of 16p11.2: deletions in mentally retarded patients without autism and in normal individuals. Eur. J. Med. Genet. 52, 77–87. doi: 10.1016/j.ejmg.2009.03.006
Blundell, J., Blaiss, C. A., Etherton, M. R., Espinosa, F., Tabuchi, K., Walz, C., et al. (2010). Neuroligin-1 deletion results in impaired spatial memory and increased repetitive behavior. J. Neurosci. 30, 2115–2129. doi: 10.1523/JNEUROSCI.4517-09.2010
Blundell, J., Tabuchi, K., Bolliger, M. F., Blaiss, C. A., Brose, N., Liu, X., et al. (2009). Increased anxiety-like behavior in mice lacking the inhibitory synapse cell adhesion molecule neuroligin 2. Genes Brain Behav. 8, 114–126. doi: 10.1111/j.1601-183X.2008.00455.x
Brown, T. I., Whiteman, A. S., Aselcioglu, I., and Stern, C. E. (2014). Structural differences in hippocampal and prefrontal gray matter volume support flexible context-dependent navigation ability. J. Neurosci. 34, 2314–2320. doi: 10.1523/JNEUROSCI.2202-13.2014
Cooper, R. A., Richter, F. R., Bays, P. M., Plaisted-Grant, K. C., Baron-Cohen, S., and Simons, J. S. (2017). Reduced hippocampal functional connectivity during episodic memory retrieval in autism. Cereb. Cortex 27, 888–902. doi: 10.1093/cercor/bhw417
Cooper, R. A., and Simons, J. S. (2019). Exploring the neurocognitive basis of episodic recollection in autism. Psychon. Bull. Rev. 26, 163–181. doi: 10.3758/s13423-018-1504-z
da Silva Montenegro, E. M., Costa, C. S., Campos, G., Scliar, M., de Almeida, T. F., Zachi, E. C., et al. (2020). Meta-analyses support previous and novel autism candidate genes: outcomes of an unexplored Brazilian cohort. Autism Res. 13, 199–206. doi: 10.1002/aur.2238
D'Amelio, M., Cavallucci, V., and Cecconi, F. (2010). Neuronal caspase-3 signaling: not only cell death. Cell Death Differ. 17, 1104–1114. doi: 10.1038/cdd.2009.180
D'Amelio, M., Cavallucci, V., Middei, S., Marchetti, C., Pacioni, S., Ferri, A., et al. (2011). Caspase-3 triggers early synaptic dysfunction in a mouse model of Alzheimer's disease. Nat. Neurosci. 14, 69–76. doi: 10.1038/nn.2709
D'Amelio, M., Sheng, M., and Cecconi, F. (2012). Caspase-3 in the central nervous system: beyond apoptosis. Trends Neurosci. 35, 700–709. doi: 10.1016/j.tins.2012.06.004
D'Angelo, D., Lebon, S., Chen, Q., Martin-Brevet, S., Snyder, L. G., Hippolyte, L., et al. (2016). Defining the effect of the 16p11.2 duplication on cognition, behavior, and medical comorbidities. JAMA Psychiatry 73, 20–30. doi: 10.1001/jamapsychiatry.2015.2123
De Rubeis, S., He, X., Goldberg, A. P., Poultney, C. S., Samocha, K., Cicek, A. E., et al. (2014). Synaptic, transcriptional and chromatin genes disrupted in autism. Nature 515, 209–215. doi: 10.1038/nature13772
Deciphering Developmental Disorders S (2015). Large-scale discovery of novel genetic causes of developmental disorders. Nature 519, 223–228. doi: 10.1038/nature14135
Deng, W., Bai, Y., Deng, F., Pan, Y., Mei, S., Zheng, Z., et al. (2022). Streptococcal pyrogenic exotoxin B cleaves GSDMA and triggers pyroptosis. Nature 602, 496–502. doi: 10.1038/s41586-021-04384-4
Djagaeva, I., and Doronkin, S. (2009). COP9 limits dendritic branching via Cullin3-dependent degradation of the actin-crosslinking BTB-domain protein Kelch. PLoS One 4:e7598. doi: 10.1371/journal.pone.0007598
Doeller, C. F., Barry, C., and Burgess, N. (2010). Evidence for grid cells in a human memory network. Nature 463, 657–661. doi: 10.1038/nature08704
Dong, Z., Chen, W., Chen, C., Wang, H., Cui, W., Tan, Z., et al. (2020). CUL3 deficiency causes social deficits and anxiety-like behaviors by impairing excitation-inhibition balance through the promotion of cap-dependent translation. Neuron 105, 475–490.e6. doi: 10.1016/j.neuron.2019.10.035
Elgersma, Y. (2015). Neurodevelopmental disease: a molecular tightrope. Nature 526, 50–51. doi: 10.1038/526050b
Erturk, A., Wang, Y., and Sheng, M. (2014). Local pruning of dendrites and spines by caspase-3-dependent and proteasome-limited mechanisms. J. Neurosci. 34, 1672–1688. doi: 10.1523/JNEUROSCI.3121-13.2014
Escamilla, C. O., Filonova, I., Walker, A. K., Xuan, Z. X., Holehonnur, R., Espinosa, F., et al. (2017). Kctd 13 deletion reduces synaptic transmission via increased rho a. Nature 551, 227–231. doi: 10.1038/nature24470
Espinosa, F., Xuan, Z., Liu, S., and Powell, C. M. (2015). Neuroligin 1 modulates striatal glutamatergic neurotransmission in a pathway and NMDAR subunit-specific manner. Front Synaptic Neurosci 7:11. doi: 10.3389/fnsyn.2015.00011
Etherton, M. R., Blaiss, C. A., Powell, C. M., and Sudhof, T. C. (2009). Mouse neurexin-1alpha deletion causes correlated electrophysiological and behavioral changes consistent with cognitive impairments. Proc. Natl. Acad. Sci. USA 106, 17998–18003. doi: 10.1073/pnas.0910297106
Fernandez, B. A., Roberts, W., Chung, B., Weksberg, R., Meyn, S., Szatmari, P., et al. (2010). Phenotypic spectrum associated with de novo and inherited deletions and duplications at 16p11.2 in individuals ascertained for diagnosis of autism spectrum disorder. J. Med. Genet. 47, 195–203. doi: 10.1136/jmg.2009.069369
Fieblinger, T., Li, C., Espa, E., and Cenci, M. A. (2022). Non-apoptotic Caspase-3 activation mediates early synaptic dysfunction of indirect pathway neurons in the parkinsonian striatum. Int. J. Mol. Sci. 23:5470. doi: 10.3390/ijms23105470
Fischer, S., Schlotthauer, I., Kizner, V., Macartney, T., Dorner-Ciossek, C., and Gillardon, F. (2020). Loss-of-function mutations of CUL3, a high confidence gene for psychiatric disorders, Lead to aberrant neurodevelopment in human induced pluripotent stem cells. Neuroscience 448, 234–254. doi: 10.1016/j.neuroscience.2020.08.028
Fortin, N. J., Agster, K. L., and Eichenbaum, H. B. (2002). Critical role of the hippocampus in memory for sequences of events. Nat. Neurosci. 5, 458–462. doi: 10.1038/nn834
Geschwind, D. H., and State, M. W. (2015). Gene hunting in autism spectrum disorder: on the path to precision medicine. Lancet Neurol. 14, 1109–1120. doi: 10.1016/S1474-4422(15)00044-7
Grabrucker, A., Vaida, B., Bockmann, J., and Boeckers, T. M. (2009). Synaptogenesis of hippocampal neurons in primary cell culture. Cell Tissue Res. 338, 333–341. doi: 10.1007/s00441-009-0881-z
Gu, Q., Jiao, S., Duan, K., Wang, Y. X., Petralia, R. S., and Li, Z. (2021). The BAD-BAX-Caspase-3 Cascade modulates synaptic vesicle pools via autophagy. J. Neurosci. 41, 1174–1190. doi: 10.1523/JNEUROSCI.0969-20.2020
Guo, J., Ji, Y., Ding, Y., Jiang, W., Sun, Y., Lu, B., et al. (2016). BDNF pro-peptide regulates dendritic spines via caspase-3. Cell Death Dis. 7:e2264. doi: 10.1038/cddis.2016.166
Han, E. B., and Stevens, C. F. (2009). Development regulates a switch between post- and presynaptic strengthening in response to activity deprivation. Proc. Natl. Acad. Sci. USA 106, 10817–10822. doi: 10.1073/pnas.0903603106
Harris, K. M., Jensen, F. E., and Tsao, B. (1992). Three-dimensional structure of dendritic spines and synapses in rat hippocampus (CA1) at postnatal day 15 and adult ages: implications for the maturation of synaptic physiology and long-term potentiation. J. Neurosci. 12, 2685–2705. doi: 10.1523/JNEUROSCI.12-07-02685.1992
Hering, H., and Sheng, M. (2001). Dendritic spines: structure, dynamics and regulation. Nat. Rev. Neurosci. 2, 880–888. doi: 10.1038/35104061
Hitti, F. L., and Siegelbaum, S. A. (2014). The hippocampal CA2 region is essential for social memory. Nature 508, 88–92. doi: 10.1038/nature13028
Hollville, E., and Deshmukh, M. (2018). Physiological functions of non-apoptotic caspase activity in the nervous system. Semin. Cell Dev. Biol. 82, 127–136. doi: 10.1016/j.semcdb.2017.11.037
Huang, H. S., Allen, J. A., Mabb, A. M., King, I. F., Miriyala, J., Taylor-Blake, B., et al. (2012). Topoisomerase inhibitors unsilence the dormant allele of Ube 3a in neurons. Nature 481, 185–189. doi: 10.1038/nature10726
Huang, Y., Huang, X., Cheng, C., Xu, X., Liu, H., Yang, X., et al. (2019). Elucidating the expression and function of Numbl during cell adhesion-mediated drug resistance (CAM-DR) in multiple myeloma (MM). BMC Cancer 19:1269. doi: 10.1186/s12885-019-6446-y
Iossifov, I., O'Roak, B. J., Sanders, S. J., Ronemus, M., Krumm, N., Levy, D., et al. (2014). The contribution of de novo coding mutations to autism spectrum disorder. Nature 515, 216–221. doi: 10.1038/nature13908
Jacobs, J., Weidemann, C. T., Miller, J. F., Solway, A., Burke, J. F., Wei, X. X., et al. (2013). Direct recordings of grid-like neuronal activity in human spatial navigation. Nat. Neurosci. 16, 1188–1190. doi: 10.1038/nn.3466
Jaramillo, T. C., Speed, H. E., Xuan, Z., Reimers, J. M., Escamilla, C. O., Weaver, T. P., et al. (2017). Novel Shank3 mutant exhibits behaviors with face validity for autism and altered striatal and hippocampal function. Autism Res. 10, 42–65. doi: 10.1002/aur.1664
Jiang, M., Qi, L., Li, L., and Li, Y. (2020). The caspase-3/GSDME signal pathway as a switch between apoptosis and pyroptosis in cancer. Cell Death Discov 6:112. doi: 10.1038/s41420-020-00349-0
Jin, Z., Li, Y., Pitti, R., Lawrence, D., Pham, V. C., Lill, J. R., et al. (2009). Cullin3-based polyubiquitination and p62-dependent aggregation of caspase-8 mediate extrinsic apoptosis signaling. Cell 137, 721–735. doi: 10.1016/j.cell.2009.03.015
Kaplan, Y., Gibbs-Bar, L., Kalifa, Y., Feinstein-Rotkopf, Y., and Arama, E. (2010). Gradients of a ubiquitin E3 ligase inhibitor and a caspase inhibitor determine differentiation or death in spermatids. Dev. Cell 19, 160–173. doi: 10.1016/j.devcel.2010.06.009
Kemper, T. L., and Bauman, M. L. (1993). The contribution of neuropathologic studies to the understanding of autism. Neurol. Clin. 11, 175–187. doi: 10.1016/S0733-8619(18)30176-2
Khatri, N., Gilbert, J. P., Huo, Y., Sharaflari, R., Nee, M., Qiao, H., et al. (2018). The autism protein Ube3A/E6AP remodels neuronal dendritic Arborization via caspase-dependent microtubule destabilization. J. Neurosci. 38, 363–378. doi: 10.1523/JNEUROSCI.1511-17.2017
Kong, A., Frigge, M. L., Masson, G., Besenbacher, S., Sulem, P., Magnusson, G., et al. (2012). Rate of de novo mutations and the importance of father's age to disease risk. Nature 488, 471–475. doi: 10.1038/nature11396
Kouser, M., Speed, H. E., Dewey, C. M., Reimers, J. M., Widman, A. J., Gupta, N., et al. (2013). Loss of predominant shank 3 isoforms results in hippocampus-dependent impairments in behavior and synaptic transmission. J. Neurosci. 33, 18448–18468. doi: 10.1523/JNEUROSCI.3017-13.2013
Kumar, R. A., Kara Mohamed, S., Sudi, J., Conrad, D. F., Brune, C., Badner, J. A., et al. (2008). Recurrent 16p11.2 microdeletions in autism. Hum. Mol. Genet. 17, 628–638. doi: 10.1093/hmg/ddm376
Kumar, V., Zhang, M. X., Swank, M. W., Kunz, J., and Wu, G. Y. (2005). Regulation of dendritic morphogenesis by Ras-PI3K-Akt-mTOR and Ras-MAPK signaling pathways. J. Neurosci. 25, 11288–11299. doi: 10.1523/JNEUROSCI.2284-05.2005
Kwon, C. H., Luikart, B. W., Powell, C. M., Zhou, J., Matheny, S. A., Zhang, W., et al. (2006). Pten regulates neuronal arborization and social interaction in mice. Neuron 50, 377–388. doi: 10.1016/j.neuron.2006.03.023
Lawrence, Y. A., Kemper, T. L., Bauman, M. L., and Blatt, G. J. (2010). Parvalbumin-, calbindin-, and calretinin-immunoreactive hippocampal interneuron density in autism. Acta Neurol. Scand. 121, 99–108. doi: 10.1111/j.1600-0404.2009.01234.x
Lee, A. C., Brodersen, K. H., and Rudebeck, S. R. (2013). Disentangling spatial perception and spatial memory in the hippocampus: a univariate and multivariate pattern analysis fMRI study. J. Cogn. Neurosci. 25, 534–546. doi: 10.1162/jocn_a_00301
Li, Z., Jo, J., Jia, J. M., Lo, S. C., Whitcomb, D. J., Jiao, S., et al. (2010). Caspase-3 activation via mitochondria is required for long-term depression and AMPA receptor internalization. Cell 141, 859–871. doi: 10.1016/j.cell.2010.03.053
Lin, P., Yang, J., Wu, S., Ye, T., Zhuang, W., Wang, W., et al. (2023). Current trends of high-risk gene Cul 3 in neurodevelopmental disorders. Front. Psych. 14:1215110. doi: 10.3389/fpsyt.2023.1215110
Lord, C., Elsabbagh, M., Baird, G., and Veenstra-Vanderweele, J. (2018). Autism spectrum disorder. Lancet 392, 508–520. doi: 10.1016/S0140-6736(18)31129-2
Marshall, C. R., Noor, A., Vincent, J. B., Lionel, A. C., Feuk, L., Skaug, J., et al. (2008). Structural variation of chromosomes in autism spectrum disorder. Am. J. Hum. Genet. 82, 477–488. doi: 10.1016/j.ajhg.2007.12.009
McCarthy, S. E., Makarov, V., Kirov, G., Addington, A. M., McClellan, J., Yoon, S., et al. (2009). Microduplications of 16p11.2 are associated with schizophrenia. Nat. Genet. 41, 1223–1227. doi: 10.1038/ng.474
McEvoy, J. D., Kossatz, U., Malek, N., and Singer, J. D. (2007). Constitutive turnover of cyclin E by Cul 3 maintains quiescence. Mol. Cell. Biol. 27, 3651–3666. doi: 10.1128/MCB.00720-06
Meira, T., Leroy, F., Buss, E. W., Oliva, A., Park, J., and Siegelbaum, S. A. (2018). A hippocampal circuit linking dorsal CA2 to ventral CA1 critical for social memory dynamics. Nat. Commun. 9:4163. doi: 10.1038/s41467-018-06501-w
Miller-Fleming, T. W., Petersen, S. C., Manning, L., Matthewman, C., Gornet, M., Beers, A., et al. (2016). The DEG/ENaC cation channel protein UNC-8 drives activity-dependent synapse removal in remodeling GABAergic neurons. eLife 5:5. doi: 10.7554/eLife.14599
Morandell, J., Schwarz, L. A., Basilico, B., Tasciyan, S., Dimchev, G., Nicolas, A., et al. (2021). Cul 3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. Nat. Commun. 12:3058. doi: 10.1038/s41467-021-23123-x
Nakashima, M., Kato, M., Matsukura, M., Kira, R., Ngu, L. H., Lichtenbelt, K. D., et al. (2020). De novo variants in CUL3 are associated with global developmental delays with or without infantile spasms. J. Hum. Genet. 65, 727–734. doi: 10.1038/s10038-020-0758-2
O'Roak, B. J., Deriziotis, P., Lee, C., Vives, L., Schwartz, J. J., Girirajan, S., et al. (2011). Exome sequencing in sporadic autism spectrum disorders identifies severe de novo mutations. Nat. Genet. 43, 585–589. doi: 10.1038/ng.835
O'Roak, B. J., Vives, L., Fu, W., Egertson, J. D., Stanaway, I. B., Phelps, I. G., et al. (2012b). Multiplex targeted sequencing identifies recurrently mutated genes in autism spectrum disorders. Science 338, 1619–1622. doi: 10.1126/science.1227764
O'Roak, B. J., Vives, L., Girirajan, S., Karakoc, E., Krumm, N., Coe, B. P., et al. (2012a). Sporadic autism exomes reveal a highly interconnected protein network of de novo mutations. Nature 485, 246–250. doi: 10.1038/nature10989
Rapanelli, M., Tan, T., Wang, W., Wang, X., Wang, Z. J., Zhong, P., et al. (2019). Behavioral, circuitry, and molecular aberrations by region-specific deficiency of the high-risk autism gene Cul 3. Mol. Psychiatry 26, 1491–1504. doi: 10.1038/s41380-019-0498-x
Raymond, G. V., Bauman, M. L., and Kemper, T. L. (1996). Hippocampus in autism: a Golgi analysis. Acta Neuropathol. 91, 117–119. doi: 10.1007/s004010050401
Rochefort, N. L., and Konnerth, A. (2012). Dendritic spines: from structure to in vivo function. EMBO Rep. 13, 699–708. doi: 10.1038/embor.2012.102
Rubin, R. D., Watson, P. D., Duff, M. C., and Cohen, N. J. (2014). The role of the hippocampus in flexible cognition and social behavior. Front. Hum. Neurosci. 8:742. doi: 10.3389/fnhum.2014.00742
Salinas, G. D., Blair, L. A., Needleman, L. A., Gonzales, J. D., Chen, Y., Li, M., et al. (2006). Actinfilin is a Cul3 substrate adaptor, linking GluR6 kainate receptor subunits to the ubiquitin-proteasome pathway. J. Biol. Chem. 281, 40164–40173. doi: 10.1074/jbc.M608194200
Sanders, S. J., He, X., Willsey, A. J., Ercan-Sencicek, A. G., Samocha, K. E., Cicek, A. E., et al. (2015). Insights into autism Spectrum disorder genomic architecture and biology from 71 risk loci. Neuron 87, 1215–1233. doi: 10.1016/j.neuron.2015.09.016
Saric, N., Hashimoto-Torii, K., Jevtovic-Todorovic, V., and Ishibashi, N. (2022). Nonapoptotic caspases in neural development and in anesthesia-induced neurotoxicity. Trends Neurosci. 45, 446–458. doi: 10.1016/j.tins.2022.03.007
Schafer, M., and Schiller, D. (2018). The Hippocampus and social impairment in psychiatric disorders. Cold Spring Harb. Symp. Quant. Biol. 83, 105–118. doi: 10.1101/sqb.2018.83.037614
Schleich, K., and Lavrik, I. N. (2013). Mathematical modeling of apoptosis. Cell Commun. Signal 11:44. doi: 10.1186/1478-811X-11-44
Shen, H., Zhu, H., Panja, D., Gu, Q., and Li, Z. (2020). Autophagy controls the induction and developmental decline of NMDAR-LTD through endocytic recycling. Nat. Commun. 11:2979. doi: 10.1038/s41467-020-16794-5
Shinawi, M., Liu, P., Kang, S. H., Shen, J., Belmont, J. W., Scott, D. A., et al. (2010). Recurrent reciprocal 16p11.2 rearrangements associated with global developmental delay, behavioural problems, dysmorphism, epilepsy, and abnormal head size. J. Med. Genet. 47, 332–341. doi: 10.1136/jmg.2009.073015
Singer, J. D., Gurian-West, M., Clurman, B., and Roberts, J. M. (1999). Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells. Genes Dev. 13, 2375–2387. doi: 10.1101/gad.13.18.2375
Siveen, K. S., Mustafa, N., Li, F., Kannaiyan, R., Ahn, K. S., Kumar, A. P., et al. (2014). Thymoquinone overcomes chemoresistance and enhances the anticancer effects of bortezomib through abrogation of NF-kappa B regulated gene products in multiple myeloma xenograft mouse model. Oncotarget 5, 634–648. doi: 10.18632/oncotarget.1596
Steinberg, S., de Jong, S., Mattheisen, M., Costas, J., Demontis, D., Jamain, S., et al. (2014). Common variant at 16p11.2 conferring risk of psychosis. Mol. Psychiatry 19, 108–114. doi: 10.1038/mp.2012.157
Tabuchi, K., Blundell, J., Etherton, M. R., Hammer, R. E., Liu, X., Powell, C. M., et al. (2007). A neuroligin-3 mutation implicated in autism increases inhibitory synaptic transmission in mice. Science 318, 71–76. doi: 10.1126/science.1146221
Tavares, R. M., Mendelsohn, A., Grossman, Y., Williams, C. H., Shapiro, M., Trope, Y., et al. (2015). A map for social navigation in the human brain. Neuron 87, 231–243. doi: 10.1016/j.neuron.2015.06.011
Varghese, M., Keshav, N., Jacot-Descombes, S., Warda, T., Wicinski, B., Dickstein, D. L., et al. (2017). Autism spectrum disorder: neuropathology and animal models. Acta Neuropathol. 134, 537–566. doi: 10.1007/s00401-017-1736-4
Wang, J. Y., Chen, F., Fu, X. Q., Ding, C. S., Zhou, L., Zhang, X. H., et al. (2014). Caspase-3 cleavage of dishevelled induces elimination of postsynaptic structures. Dev. Cell 28, 670–684. doi: 10.1016/j.devcel.2014.02.009
Wang, J., and Lenardo, M. J. (2000). Roles of caspases in apoptosis, development, and cytokine maturation revealed by homozygous gene deficiencies. J. Cell Sci. 113, 753–757. doi: 10.1242/jcs.113.5.753
Wegiel, J., Kuchna, I., Nowicki, K., Imaki, H., Wegiel, J., Marchi, E., et al. (2010). The neuropathology of autism: defects of neurogenesis and neuronal migration, and dysplastic changes. Acta Neuropathol. 119, 755–770. doi: 10.1007/s00401-010-0655-4
Weiss, L. A., Shen, Y., Korn, J. M., Arking, D. E., Miller, D. T., Fossdal, R., et al. (2008). Association between microdeletion and microduplication at 16p11.2 and autism. N. Engl. J. Med. 358, 667–675. doi: 10.1056/NEJMoa075974
Xia, Q. Q., Walker, A. K., Song, C., Wang, J., Singh, A., Mobley, J. A., et al. (2023). Effects of heterozygous deletion of autism-related gene Cullin-3 in mice. PLoS One 18:e0283299. doi: 10.1371/journal.pone.0283299
Xu, J., Xiao, N., and Xia, J. (2010). Thrombospondin 1 accelerates synaptogenesis in hippocampal neurons through neuroligin 1. Nat. Neurosci. 13, 22–24. doi: 10.1038/nn.2459
Yi, J. J., Berrios, J., Newbern, J. M., Snider, W. D., Philpot, B. D., Hahn, K. M., et al. (2015). An autism-linked mutation disables phosphorylation control of UBE3A. Cell 162, 795–807. doi: 10.1016/j.cell.2015.06.045
Zhou, J., Blundell, J., Ogawa, S., Kwon, C. H., Zhang, W., Sinton, C., et al. (2009). Pharmacological inhibition of mTORC1 suppresses anatomical, cellular, and behavioral abnormalities in neural-specific Pten knock-out mice. J. Neurosci. 29, 1773–1783. doi: 10.1523/JNEUROSCI.5685-08.2009
Zhu, S., Perez, R., Pan, M., and Lee, T. (2005). Requirement of Cul3 for axonal arborization and dendritic elaboration in Drosophila mushroom body neurons. J. Neurosci. 25, 4189–4197. doi: 10.1523/JNEUROSCI.0149-05.2005
Keywords: cullin-3, dendrite, axon, spine, synapse, caspase-3
Citation: Xia Q-q, Singh A, Wang J, Xuan ZX, Singer JD and Powell CM (2024) Autism risk gene Cul3 alters neuronal morphology via caspase-3 activity in mouse hippocampal neurons. Front. Cell. Neurosci. 18:1320784. doi: 10.3389/fncel.2024.1320784
Edited by:
Zhanyan Fu, Broad Institute, United StatesReviewed by:
Andrzej W. Cwetsch, Instituto de Biotecnología y Biomedicina Universidad de Valencia, SpainGülcan Akgül, Cornell University, United States
Copyright © 2024 Xia, Singh, Wang, Xuan, Singer and Powell. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Craig M. Powell, craigpow@uab.edu