AUTHOR=Schubert Charlotte , Schulz Kristina , Träger Simone , Plath Anna-Lena , Omriouate Asina , Rosenkranz Sina C. , Morellini Fabio , Friese Manuel A. , Hirnet Daniela 

TITLE=Neuronal Adenosine A1 Receptor is Critical for Olfactory Function but Unable to Attenuate Olfactory Dysfunction in Neuroinflammation

JOURNAL=Frontiers in Cellular Neuroscience

VOLUME=16

YEAR=2022

URL=https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2022.912030

DOI=10.3389/fncel.2022.912030

ISSN=1662-5102

ABSTRACT=<p>Adenine nucleotides, such as adenosine triphosphate (ATP), adenosine diphosphate (ADP), as well as the nucleoside adenosine are important modulators of neuronal function by engaging P1 and P2 purinergic receptors. In mitral cells, signaling of the G protein-coupled P1 receptor adenosine 1 receptor (A<sub>1</sub>R) affects the olfactory sensory pathway by regulating high voltage-activated calcium channels and two-pore domain potassium (K2P) channels. The inflammation of the central nervous system (CNS) impairs the olfactory function and gives rise to large amounts of extracellular ATP and adenosine, which act as pro-inflammatory and anti-inflammatory mediators, respectively. However, it is unclear whether neuronal A<sub>1</sub>R in the olfactory bulb modulates the sensory function and how this is impacted by inflammation. Here, we show that signaling <italic>via</italic> neuronal A<sub>1</sub>R is important for the physiological olfactory function, while it cannot counteract inflammation-induced hyperexcitability and olfactory deficit. Using neuron-specific A<sub>1</sub>R-deficient mice in patch-clamp recordings, we found that adenosine modulates spontaneous dendro-dendritic signaling in mitral and granule cells <italic>via</italic> A<sub>1</sub>R. Furthermore, neuronal A<sub>1</sub>R deficiency resulted in olfactory dysfunction in two separate olfactory tests. In mice with experimental autoimmune encephalomyelitis (EAE), we detected immune cell infiltration and microglia activation in the olfactory bulb as well as hyperexcitability of mitral cells and olfactory dysfunction. However, neuron-specific A<sub>1</sub>R activity was unable to attenuate glutamate excitotoxicity in the primary olfactory bulb neurons <italic>in vitro</italic> or EAE-induced olfactory dysfunction and disease severity <italic>in vivo</italic>. Together, we demonstrate that A<sub>1</sub>R modulates the dendro-dendritic inhibition (DDI) at the site of mitral and granule cells and impacts the processing of the olfactory sensory information, while A<sub>1</sub>R activity was unable to counteract inflammation-induced hyperexcitability.</p>