Differential expression of metabotropic glutamate and GABA receptors at neocortical glutamatergic and GABAergic axon terminals

Metabotropic glutamate (Glu) receptors (mGluRs) and GABA_B receptors are highly expressed at presynaptic sites. To verify the possibility that the two classes of metabotropic receptors contribute to axon terminals heterogeneity, we studied the localization of mGluR1α, mGluR5, mGluR2/3, mGluR7, and GABA_B1 in VGLUT1-, VGLUT2-, and VGAT- positive terminals in the cerebral cortex of adult rats. VGLUT1-positive puncta expressed mGluR1α (∼5%), mGluR5 (∼6%), mGluR2/3 (∼22%), mGluR7 (∼17%), and GABA_B1 (∼40%); VGLUT2-positive terminals expressed mGluR1α (∼10%), mGluR5 (∼11%), mGluR2/3 (∼20%), mGluR7 (∼28%), and GABA_B1 (∼25%); whereas VGAT-positive puncta expressed mGluR1α (∼27%), mGluR5 (∼24%), mGluR2/3 (∼38%), mGluR7 (∼31%), and GABA_B1 (∼19%). Control experiments ruled out the possibility that postsynaptic mGluRs and GABA_B1 might have significantly biased our results. We also performed functional assays in synaptosomal preparations, and showed that all agonists modify Glu and GABA levels, which return to baseline upon exposure to antagonists. Overall, these findings indicate that mGluR1α, mGluR5, mGluR2/3, mGluR7, and GABA_B1 expression differ significantly between glutamatergic and GABAergic axon terminals, and that the robust expression of heteroreceptors may contribute to the homeostatic regulation of the balance between excitation and inhibition.

Introduction

Presynaptic mechanisms affecting neurotransmitter release play a key role in modulating synaptic strength and plasticity (Atwood and Karunanithi, 2002); they may be associated to molecular heterogeneity of axon terminals (Staple et al., 2000). Following the demonstration that more than 2,000 genes are differentially expressed in glutamatergic and GABAergic neurons (Sugino et al., 2006), we investigated whether glutamatergic and GABAergic release machineries could be differentiated on the basis of the proteins they express, and showed that in cerebral cortex the expression pattern of several presynaptic proteins involved in transmitter release (including synapsins, synaptophysins, synaptosomal-associated proteins, synaptogyrins, synaptobrevin/vesicle-associated membrane proteins, syntaxins, synaptotagmins, synaptic vesicle proteins, and Rab3) varies both between glutamatergic and GABAergic terminals and between VGLUT1+ and VGLUT2+ glutamatergic terminals (Bragina et al., 2007, 2010, 2011).

Metabotropic glutamate (Glu) receptors (mGluRs) and GABA_B modulates neuronal excitability, transmitter release, and synaptic plasticity (Bowery et al., 1980; Herrero et al., 1992; Pin and Duvoisin, 1995). mGluRs consist of at least eight subtypes classified into three groups: group I receptors (mGluR1 and 5), coupled to the Gq signal pathway, are predominantly postsynaptic, and generally potentiate neuronal excitability; group II (mGluR2 and 3) and group III receptors (mGluR 4, 6, 7, and 8), linked to Gi/Go and thus cAMP inhibitors, are predominantly presynaptic and usually decrease excitability (Shigemoto et al., 1997; Gereau and Swanson, 2008). Notwithstanding the value of this general scheme, it is worth noting that the localization of mGluRs is more complex. Indeed, besides mGluR2/3 and mGluR7, mGluR1α and mGluR5 are also expressed presynaptically at asymmetric and symmetric synapses in different brain regions, including cerebral cortex (Romano et al., 1995; Bradley et al., 1996; Petralia et al., 1996; Lujan et al., 1997; Shigemoto et al., 1997; Kinoshita et al., 1998; Tamaru et al., 2001; Dalezios et al., 2002; Muly et al., 2003; Paquet and Smith, 2003; Somogyi et al., 2003; Rodrigues et al., 2005; Musante et al., 2008; Giribaldi et al., 2013), where they also modulate positively or negatively glutamate and GABA release (Herrero et al., 1992; Battaglia et al., 1997; Li et al., 2008; Musante et al., 2008; Summa et al., 2013).

GABA_B are coupled to Gi/Go proteins and decrease neuronal excitability in both pre- and postsynaptic elements; they exist as heterodimers (of GABA_B1 and GABA_B2 subunits) which yield functional receptors (Kaupmann et al., 1998; White et al., 1998; Gonchar et al., 2001; Lopez-Bendito et al., 2002; Tabata et al., 2004). GABA_B1 is expressed presynaptically in several brain regions (Kulik et al., 2002, 2003; Lujan et al., 2004; McDonald et al., 2004; Lacey et al., 2005; Lujan and Shigemoto, 2006), including the cerebral cortex, where it is localized at both symmetric and asymmetric synapses (Gonchar et al., 2001; Lopez-Bendito et al., 2002). One major function of presynaptic GABA_B receptors is modulation of neurotransmitter release, as they inhibit the release of both glutamate and GABA in different regions of the CNS, including cerebral cortex (Bowery et al., 1980; Bonanno and Raiteri, 1993).

The widespread localization of mGluRs and GABA_B at presynaptic sites raises the possibility that both classes of metabotropic receptors contribute to axon terminals heterogeneity. To verify this hypothesis, we studied the localization of mGluR1α, mGluR5, mGluR2/3, mGluR7, and GABA_B1 in VGLUT1+, VGLUT2+, and VGAT+¹ terminals in the cerebral cortex of adult rats. We report that the expression of these receptors differs significantly between glutamatergic and GABAergic axon terminals, and that expression of heteroreceptors is greater than expected. We also performed functional assays in synaptosomal preparations, and showed that all agonists modify Glu and GABA levels, which return to baseline upon antagonists administration.

Materials and Methods

Animals and Tissue Preparation

Adult male Sprague–Dawley rats (190–220 g; Charles River, Milan, Italy) were used. All experiments were carried out in accordance with the European Community Council Directive dated November 24, 1986 (86/609 EEC), and were approved by the local authority veterinary service (CESA, Comitato Etico per la Sperimentazione Animale; Università Politecnica delle Marche). Animals were kept under a dark–light cycle of 12 h and permitted food and water ad libitum.

For synaptosome purification, release experiments, and western blotting, rats were euthanized and brains rapidly removed. Homogenization of neocortex, membrane preparation, protein determination, SDS-PAGE analysis, and immunoblotting were performed as described (Bragina et al., 2006). Precast gels (Tris-HCl; BioRad, Hercules, CA, USA) were used at 4–20% polyacrylamide concentration for metabotropic receptors (15 μg total proteins) and at 7.5% for the vesicular transporters (10 μg total proteins).

For immunocytochemical studies, rats were anesthetized with chloral hydrate (300 mg/kg i.p.) and perfused through the ascending aorta with saline followed by 4% paraformaldehyde in 0.1 M phosphate buffer (PB; pH 7.4). Brains were post-fixed for 1 day at 4°C in the same fixative, cut with a Vibratome into 30 μm thick sections, and processed.

Antibodies

The primary antibodies used are listed in Table 1. Western blots were performed to verify antibodies specificity; nitrocellulose filters were probed with antibodies to VGLUT1, VGLUT2, VGAT, mGluR1α, mGluR5, mGluR2/3, mGluR7, and GABA_B1 at the dilutions reported in Table 1. After exposure to the appropriate peroxidase-conjugated antibodies (Vector; Burlingame, CA, USA), immunoreactive bands were visualized by BioRad Chemidoc and Quantity One software (BioRad, Hemel Hempstead, UK) using the SuperSignal West Pico (Rockford, IL, USA) chemiluminescent substrate.

TABLE 1

TABLE 1. Primary antibodies.

Co-localization Studies

Sections were incubated for 1 h in normal goat serum (NGS; 10% in PB with 0.2% Triton X-100), and then overnight at room temperature in a solution containing a mixture of the primary antibodies. The next day, sections were incubated in 10% NGS (30 min), and then for 90 min in a mixture of the appropriate secondary fluorescent antibodies. For the VGLUT1 and VGLUT2 series, we used fluorescein isothiocyanate-conjugated goat anti-guinea-pig IgG (FI-7000, Vector; Burlingame, CA, USA) for the vesicular transporters (1:100) and tetramethylrhodamine isothiocyanate-conjugated goat anti-rabbit IgG (T-2769, Molecular Probes; Poort Gebouw, The Netherlands) for the metabotropic receptors (1:100); for the VGAT series, we used fluorescein goat anti-mouse IgG (F-2761, Molecular probes) for the vesicular transporter (1:100) and tetramethylrhodamine isothiocyanate-conjugated goat anti-rabbit IgG (T-2769, Molecular Probes; Poort Gebouw, The Netherlands) for the metabotropic receptors (1:100). Sections were then mounted, air-dried, and coverslipped using Vectashield mounting medium (H-1000; Vector). Double-labeled sections were examined using a Leica (TCS SP2) confocal laser microscope equipped with an argon (488 nm) and a helium/neon (543 nm) laser for excitation of fluorescein isothiocyanate (FITC) and tetramethylrhodamine isothiocyanate (TRITC), respectively. Green and red immunofluorescence were imaged sequentially and emissions separated with 515/30 nm band pass (FITC) and 570 nm long pass (TRITC) filters. Control experiments with single-labeled sections and sections incubated either with two primary and one secondary antibody, or with one primary and two secondary antibodies revealed no appreciable FITC/TRITC bleed-through or antibody cross-reactivity.

Images of experimental series were collected from a region of the parietal cortex characterized by a conspicuous layer IV, with intermingled dysgranular regions, densely packed layers II and III, and a relatively cell-free layer Va. This area corresponds to the first somatic sensory cortex (SI), as identified also by Woolsey and LeMessurier (1948), Welker (1971), Zilles et al. (1980), Donoghue and Wise (1982). Images were acquired from randomly selected subfields in layers II–VI (at least 4–6/layer; 2–4 sections/animal; 10 rats). Layer I was not sampled because it hardly contains VGAT+ puncta (Chaudhry et al., 1998; Minelli et al., 2003). Images were acquired using a 63 × oil immersion lens (numerical aperture 1.4; pinhole 1.0 and image size 1,024 × 1,024 pixels, yielding a pixel size of 0.06 μm) from a plane in which the resolution of both stains was optimal and always between 1.3 and 1.8 μm from the surface. To improve the signal/noise ratio, 10 frames/image were averaged.

Quantitative analysis was performed in ∼8,000 randomly selected subfields measuring about 25 × 25 μm from the 1,024 × 1,024 pixel images. In order to minimize the fusion of puncta, the contrast of each image was adjusted manually within the maximum range of levels for each color channel. Analysis of contrast adjustment (not shown) showed that gain/contrast changes within the spectrum used did not alter significantly the percentage of puncta. Then, without reducing the image resolution, the images were binarized and processed by watershed filter using ImageJ software (bfd). Next, each channel was examined separately to identify and count with ImageJ immunopositive puncta; the two channels were then merged and the number of co-localizing puncta was counted manually. Puncta were considered double-labeled when overlap was virtually complete or when a given punctum was entirely included in the other. Moreover, we analyzed ∼2,000 randomly selected subfield (25 × 25 μm) from the 1,024 × 1,024 pixel images acquired in molecular layer of cerebellum and ventrobasal nucleus (10–20/section; 2–4section/animal; 2 animals).

In addition, we compared our manual method with a computerized overlap analysis that defines two objects as co-localized if the centre of mass of one falls within the area of the other (Lachmanovich et al., 2003). To this end, we analyzed about half of all double-labeled sections studied here with the overlap method included in JACoP toolbox of ImageJ (Bolte and Cordelieres, 2006), and found that the percentage of co-localization obtained with the two methods were comparable.

Synaptosomes Purification

Synaptosomes were prepared from rat neocortex with a protocol modified by Dunkley et al. (1986) and Stigliani et al. (2006). Briefly, rats were sacrificed and brain were rapidly removed. Parietal cortices were homogenized in 10 volume of Tris buffer (4°C; pH 7.4) containing 0.32 M sucrose, EDTA 1 mM and protease inhibitors (Complete EDTA-free; Roche Molecular Biochemicals, Indianapolis, IN, USA), and centrifuged at 1,000 g for 5 min to remove nuclei and cellular debris. Subsequently, supernatant was centrifuged at 9,200 g for 10 min. Synaptosomal fraction were purified by centrifugation a 33,000 g using Percoll-sucrose density gradient (2–6–10–20%) for 5 min. The synaptosomal fraction (10–20%) Percol interface was washed by centrifugation at 20,000 g for 15 min at 4°C, and resuspended in fresh physiologic medium having the following composition (in mM): 140 NaCl, 3 KCl, 1.2 MgSO₄, 1.2 NaH₂PO₄, 5 NaHCO₃, 1.2 CaCl₂; 10 Hepes, and 10 glucose (pH 7.4) for release experiments.

Release Experiments

Synaptosomes (from 32 rats) were incubated at 37°C for 15 min; aliquots of synaptosomal suspension (150 μg) were layered on microporous filters placed at the bottom of a set of parallel superfusion chambers maintained at 37°C (Superfusion System; Ugo Basile, Comerio, Italy; (Raiteri et al., 1984). Superfusion was started with standard medium at a rate of 0.5 ml/min and continued for 48 min. In the experiments aimed at measuring basal Glu and GABA release, after 36 min of superfusion to equilibrate the system, fractions were collected according to the following scheme: four 3-min samples (t = 36–39, basal release; t = 39–42, t = 42–45, and t = 45–48, drug-induced release). The mGluR1 and mGluR5 agonist 3,5-DHPG (30 μM) was introduced at t = 39, after the first sample was collected. When appropriate, the selective mGluR1 and mGluR5 antagonists LY367385 (1 μM) and MPEP (1 μM), respectively, were introduced at t = 30 and maintained until the end of the experiment. Drug effects were evaluated by comparing the amount of endogenous Glu or GABA (pmol/mg synaptosomal protein) in the fourth sample collected (in which maximum effect of 3,5-DHPG was generally reached) with the content in the first sample (basal efflux). In experiments aimed at measuring the stimulus-evoked Glu and GABA release, after 36 min of superfusion to equilibrate the system, fractions were collected according to the following scheme: two 3-min samples (t = 36–39 min and t = 45–48 min; basal outflow) before and after one 6-min sample (t = 39–45 min; stimulus-evoked release). A 90-s period of stimulation was applied at t = 39 min, after the collection of the first sample. Stimulation of synaptosomes was performed by 15 mM KCl, substituting for equimolar concentration of NaCl. The mGluR2 and mGluR3 agonist LY379268 (100 nM), the mGluR7 agonist AMN082 (100 nM) or the GABA_B receptor agonist (-)baclofen (10 μM) was introduced at t = 39 min concomitantly to the KCl pulse. When appropriate, the selective mGluR2 and mGluR3 antagonist LY341495 (100 nM), the selective mGluR7 antagonist MMPIP (10 nM) or the selective GABA_B antagonist CGP52432 (1 μM) was introduced at t = 30 min and maintained until the end of the experiment. The stimulus-evoked overflow was estimated by subtracting the endogenous neurotransmitter release content of the two 3-min fractions (basal outflow) from the endogenous neurotransmitter amount collected in the 6-min fraction (stimulus-evoked overflow).

Endogenous Glu and GABA content in the samples collected was measured by high performance liquid chromatography following pre-column derivatization with o-phthalaldehyde and gradient separation on a C18 reverse-phase chromatographic column (10 mm × 4.6 mm, 3 μm; at 30°C; Chrompack, Middleburg, The Netherlands) coupled with fluorometric detection (excitation wavelength 350 nm; emission wavelength 450 nm). Homoserine was used as an internal standard (Bonanno et al., 2005). The following buffers were used: solvent A, 0.1 M sodium acetate (pH 5.8)/methanol 80:20; solvent B, 0.1 M sodium acetate (pH 5.8)/methanol, 20:80; solvent C, sodium acetate (pH 6.0)/methanol, 80:20. The gradient program was as follows: 100% C for 4 min from the initiation of the program; 90% A and 10% B in 1 min; 42% A and 58% B in 14 min; 100% B in 1 min; isocratic flow 2 min; 100% C in 3 min; flow rate 0.9 mL/min. Amino acid release content was expressed as picomoles per milligram of synaptosomal protein.

All chemicals were of the purest analytical grade. (s)-3,5-Dihydroxyphenylglycine ((s)3,5-DHPG, ab120007), 2-Methyl-6-(phenylethynyl)pyridine hydrochloride (MPEP hydrochloride, ab120008), (S)-(+)-α-Amino-4-carboxy-2-methylbenzeneacetic acid (LY367385, ab120067), (1R,4R,5S,6R)-4-amino-2-oxabicyclo[3.1.0]hexane-4,6-dicarboxylic acid (LY379268, ab120196), (2S)-2-Amino-2-[(1S,2S)-2-carboxycycloprop-1-yl]-3-(xanth-9-yl)propanoic acid (LY341495, ab120199), N,N’-Dibenzhydrylethane-1,2-diamine dihydrochloride (AMN082 dihydrochloride, ab120011), 6-(4-Methoxyphenyl)-5-methyl-3-(4-pyridinyl)isoxazolo[4,5-c]pyridin-4(5H)-one (MMPIP, ab120245) were from ABCAM (Cambridge, UK).(R)-4-Amino-3-(4-chlorophenyl)butanoic acid ((-)baclofen, code 0796) and 3-[[(3,4-Dichlorophenyl)methyl]amino]propyl] diethoxymethyl)phosphinic acid (CGP 52432, code 1246) were from Tocris Bioscience (Bristol, UK).

Statistical Analysis

Statistical significance was evaluated by non-parametric Mann–Whitney U test (for confocal microscopy in cerebellum and ventrobasal complex), and non-parametric one way ANOVA (Kruskal–Wallis with Dunn’s post-test for confocal microscopy and release experiments in cerebral cortex).

Results

In cortical crude membrane fractions, all antibodies recognized bands of the predicted molecular mass (Figure 1; Reid et al., 1995; Petralia et al., 1996; Bellocchio et al., 1998; Chaudhry et al., 1998; Kinoshita et al., 1998; Kulik et al., 2002; Varoqui et al., 2002). VGLUT1, VGLUT2, mGluR1α, mGluR5, mGluR2/3, mGluR7, and GABA_B1 immunoreactivities were as described in previous studies (Reid et al., 1995; Romano et al., 1995; Petralia et al., 1996; Bellocchio et al., 1998; Chaudhry et al., 1998; Kinoshita et al., 1998; Kaneko et al., 2002; Kulik et al., 2002; Lopez-Bendito et al., 2002; Minelli et al., 2003; Muly et al., 2003; Alonso-Nanclares et al., 2004; Conti et al., 2005). These antibodies were, therefore, used to verify whether mGluR1α, mGluR5, mGluR2/3, mGluR7, and GABA_B1 are differentially expressed in VGLUT1+, VGLUT2+, and VGAT+ axon terminals.

FIGURE 1

FIGURE 1. VGLUT1, VGLUT2, VGAT, mGluR1α, mGluR5, mGluR2/3, mGluR7, and GABA_B1 antibodies recognized bands of ∼55, 60, 57, 140, 132, 100, and 190, 97, 100, and 132 kDa in the order in crude membrane fractions of rat cerebral cortex.

Expression of mGluRs and GABA_B1 in VGLUT1+ cortical axon terminals was studied in 25 sections from eight rats (Figures 2 and 3).

FIGURE 2

FIGURE 2. Expression of metabotropic glutamate and GABA receptors in cerebral cortex. The figure shows representative images from the VGLUT1 series (first row), the VGLUT2 series (second row), and the VGAT series (third row). Puncta were considered double-labeled (arrows) when overlap was virtually complete or when a given punctum was entirely included in the other puncta. Bar: 4 μm.

FIGURE 3

FIGURE 3. Expression of mGluR1α, mGluR5, mGluR2/3, mGluR7, and GABA_B1 in VGLUT1+, VGLUT2+, and VGAT+ axon terminals in cerebral cortex. Values are mean ± SEM; ^∗∗p < 0.01, ^∗∗∗p < 0.001.

The results showed that 4.93% of VGLUT1+ puncta expressed mGluR1α, 6.31% mGluR5, 22.01% mGluR2/3, 17.23% mGluR7, and 39.59% GABA_B1 (Figure 3; Table 2). Analysis of VGLUT2+ cortical axon terminals (30 sections from 10 animals) showed that 9.74% of VGLUT2+ puncta co-localized mGluR1α, 10.55% mGluR5, 20.37% mGluR2/3, 28.03% mGluR7, and 24.79% GABA_B1 (Figure 3; Table 2). Analogous studies of VGAT+ puncta (24 sections from 8 rats) revealed that 27.18% of VGAT expressed mGluR1α, 24.20% mGluR5, 38.09% mGluR2/3, 31.39% mGluR7, and 18.94% GABA_B1 (Figure 3; Table 2). None of the proteins studied exhibited a significant differential laminar distribution.

TABLE 2

TABLE 2. mGluR1α, mGluR5, mGluR2/3, mGluR7, and GABA_B1 in VGLUT1, VGLUT2, and VGAT puncta.

Since mGluRs and GABA_B are located both pre- and postsynaptically, we tested whether our data on pre-synaptic localization had been biased by the presence of postsynaptic receptors. GABA_B1 is expressed presynaptically in cerebellum but not in the thalamic ventrobasal complex (Kulik et al., 2002); we therefore studied GABA_B1 expression in VGLUT1, VGLUT2, and VGAT+ puncta in cerebellum and ventrobasal complex (2 rats, 12 sections) to verify whether our confocal method was able to differentiate pre-synaptic from postsynaptic localization. We found that whereas in cerebellum 19 ± 1% of VGLUT1, 9.6 ± 0.3% of VGLUT2, and 9.2 ± 0.3% of VGAT were GABA_B1+, in ventrobasal complex only 4.9 ± 0.1%, of VGLUT1, 4.4 ± 0.1% of VGLUT2, and 4.2 ± 0.2% of VGAT were GABA_B1+ (Figure 4). These findings indicate that most of labeling identified as presynaptic is indeed presynaptic, even though values given in the preceding paragraph may be slightly overestimated (see Discussion).

FIGURE 4

FIGURE 4. Control analysis of confocal microscopy images. VGLUT1/GABA_B1 in molecular layer of cerebellum (Cb) and in ventrobasal complex (VB). Puncta were considered double-labeled when overlap was virtually complete or when a given punctum was entirely included in the other puncta (arrows in third and fourth columns). Puncta that did not meet these criteria (arrowheads) were not considered double-labeled; Bars: 2 μm.

To further confirm the presynaptic localization of mGluRs and GABA_B1 and to gain insights on their functional role, we studied the impact of activation of the two classes of metabotropic receptors on the release of endogenous glutamate and GABA in rat parietal cortex. The spontaneous efflux of GABA and Glu from cerebral cortex synaptosomes in superfusion, in the absence of stimulus amounted to 51.4 ± 4.62 and to 99.6 ± 4.95 pmol/mg of proteins, respectively. The 15 mM KCl-evoked GABA and Glu overflow amounted to 215.83 ± 17 and to 482.15 ± 38.2 pmol/mg of proteins, respectively. 3,5-DHPG (30 μM), an agonist of mGluR1/5 (Wisniewski and Car, 2002), increased the spontaneous outflow of Glu from rat parietal cortex synaptosomes in superfusion by 40.26 ± 7.45%; its effect was counteracted by the mGluR1 antagonist LY367385 (1 μM; Bruno et al., 1999) and the mGluR5 antagonist MPEP (1 μM; Gasparini et al., 1999; Figure 5). Similar results were obtained by measuring the potentiation by DHPG (41.60 ± 7.65%) and the antagonism by LY367385 and MPEP on the basal release of GABA (Figure 5). LY379268 (0.1 μM), a selective mGluR2/3 agonist (Monn et al., 1999), significantly reduced the 15 mM KCl-evoked overflow of Glu (by 40.23 ± 5.2%; Figure 5) and of GABA (by 30 ± 6%; Figure 5); both effects were reverted by 0.01 μM LY341495, a selective mGluR2/3 antagonist (Kingston et al., 1998). AMN082 (0.1 μM), a selective mGluR7 agonist (Mitsukawa et al., 2005), inhibited the release of Glu (by 39.08 ± 3.07%; (Figure 5) and GABA (35.3 ± 6.45%; (Figure 5) induced by 15 mM KCl; both effects were reverted by 0.01 μM MMPIP, a selective mGluR7 antagonist (Suzuki et al., 2007). Next, we analyzed the effect of (-)baclofen, a selective GABA_B receptor agonist (Bowery et al., 1980), on the release of glutamate and GABA induced by 15 mM KCl. Figure 5 shows that the release of glutamate induced by depolarization was significantly reduced (by 49.37 ± 3.39%) by 10 μM(-)baclofen. The effect of (-)baclofen was reverted by the selective GABA_B receptor antagonist CGP52432 (1 μM) (Froestl et al., 1995). (-)baclofen (10 μM) also inhibited the 15 mM KCl-evoked release of GABA (reducing it by 40.79 ± 8.12%), and its effect was counteracted by 1 μM CGP52432 (Figure 5). These studies confirm and extend the results of previous studies, by showing that presynaptic mGluRs modulate and GABA_B reduces Glu and GABA release and that the effects on the release of the two amino acid were comparable.

FIGURE 5

FIGURE 5. Effect of agonists and antagonists of mGluR1/5, mGluR2/3, mGluR7, or GABA_B receptors on the release of endogenous glutamate (A) and GABA (B) from rat parietal cortex synaptosomes. Data are expressed as percent of the spontaneous outflow (mGluR1/5 effect) or of the 15 mM KCl-evoked overflow (mGluR2/3/7 and GABA_B effects). White and gray columns refer to agonist or agonist + antagonist administration. Data are means ± SEM of 6–10 independent experiments (6–10 rats per group). ^∗p < 0.05 and ^∗∗p < 0.01 vs. controls.

Discussion

The present study showed that mGluRs and GABA_B receptors are differentially expressed in glutamatergic and GABAergic axon terminals. Interestingly, mGluR1α, mGluR5, mGluR2/3, and mGluR7 are more robustly expressed at GABAergic than at glutamatergic terminals, whereas GABA_B1 is more expressed in glutamatergic terminals. In addition, we showed that in cerebral cortex mGluR1 and mGluR5 potentiate Glu and GABA release, whereas mGluR2/3, mGluR7, and GABA_B receptors inhibit it.

As reported in Introduction, the mGluRs studied in the present investigation have a presynaptic localization. Indeed, besides mGluR2/3 – a paradigmatic presynaptic mGluR (Ohishi et al., 1994; Neki et al., 1996; Petralia et al., 1996; Lujan et al., 1997; Tamaru et al., 2001), presynaptic expression of mGluR1α, mGluR5 (which are mostly postsynaptic) and mGluR7 in cerebral cortex has been documented (Romano et al., 1995; Bradley et al., 1996; Shigemoto et al., 1996, 1997; Kinzie et al., 1997; Kinoshita et al., 1998; Kosinski et al., 1999; Dalezios et al., 2002; Muly et al., 2003; Somogyi et al., 2003). However, some of the mGluRs studied and GABA_B receptors are expressed both pre- and postsynaptically (Kulik et al., 2002; Lopez-Bendito et al., 2002; Boyes and Bolam, 2003; Kulik et al., 2003; Chen et al., 2004; Lujan et al., 2004; Lacey et al., 2005; Lujan and Shigemoto, 2006). Therefore, we performed several control studies to rule out the possibility that our “presynaptic” population had been contaminated by postsynaptic receptors. Since presynaptic localization of GABA_B1 occurs in cerebellum but not in the thalamic ventrobasal (VB) nucleus (Kulik et al., 2002), we first studied the co-localization of GABA_B1 receptor and VGLUT1, VGLUT2, and VGAT in both cerebellum and VB, and found a significant differences between co-localization in cerebellum and VB (where co-localization was very low) in the three types of terminals. It is therefore safe to assume that our confocal method can satisfactorily discriminate presynaptic and postsynaptic expression. This view is further reinforced by the observation that in our experimental conditions GABA_B1 agonists elicited modulatory responses. Next, we performed functional assays in synaptosomal preparations, which provided further evidence that the mGluRs and GABA_B studied immunocytochemically are indeed located presynaptically. To this purpose, we monitored Glu and GABA release in cerebral cortex synaptosomes under superfusion conditions (Raiteri et al., 1984). It has been demonstrated that by this way all the released transmitters/modulators are immediately removed by the superfusion medium flow, before they can interact with receptors or other proteins expressed at the presynaptic level. As a consequence, any drug-induced effect on the release of a given neurotransmitter can be attributed exclusively to the direct action of the drug at the nerve terminal storing and releasing that neurotransmitter. Thus, the modulation of Glu or GABA release reported here is in all likelihood due to activation of mGluRs or GABA_B1 receptors located at Glu or GABA releasing nerve terminals, respectively. Overall, these sets of data provide evidence that, although we might have slightly (<5%) overestimated the population of mGluRs and GABA_B receptors, our immunocytochemical localization of presynaptic receptors was not grossly biased by their postsynaptic localization. The present observation that in adult neocortex mGlu and GABA_B1 receptors exhibit differences between glutamatergic and GABAergic terminals expands the notion that these axon terminals greatly differ in their complement of presynaptic proteins (Bragina et al., 2007, 2010, 2011).

The present results show that not all glutamatergic terminals express mGluRs. Indeed, our data indicate that at least 60% of VGLUT1+ and >10% of VGLUT2+ glutamatergic terminals are devoid of glutamate-mediated metabotropic control. Moreover, these percentages could be significantly increased by the possibility that some terminals express more than one mGluR. As far as VGAT+ terminals are concerned, the present data suggest that all of them could express mGluRs, although the lack of triple-labeling studies prevent a firm conclusion, and that only a small fraction do exhibit GABA-mediated metabotropic control. Thus, although the amount of data available is still limited, it is safe to conclude that not all glutamatergic and GABAergic terminals are controlled through metabotropic receptors by glutamate and GABA, respectively.

Besides this, our results provide some novel data on mGluRs and GABA_B receptors localization in cerebral cortex. mGluRs and GABA_B receptors have been described in both glutamatergic and GABAergic terminals (Gereau and Swanson, 2008; Raiteri, 2008, for reviews), but the present quantitative observation in neocortex that presynaptic mGluRs are so strongly expressed in GABAergic and that presynaptic GABA_B receptors are more expressed in glutamatergic than in GABAergic terminals adds much to previous observations and opens a new perspective to unravel cortical microcircuits. Indeed, although the complex relationships between glutamatergic and GABAergic are only beginning to be unveiled (Lujan et al., 2005; Pinheiro and Mulle, 2008; Heja et al., 2009), making any hypothesis fragile, it is conceivable that the strong heterolocalization of mGluRs and GABA_B receptors at cortical axon terminals is part of the complex homeostatic mechanism regulating the balance between excitation and inhibition.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

This study was supported by funds from MIUR (PRIN 2012 to FC) and Università Politecnica delle Marche. We are grateful to Dr. Akos Kulik (Institute of Physiology II, University of Freiburg) for providing us with the GABA_B1 antibody, and to Drs. Fabio Benfenati and Federica Bosco (IIT and University of Genoa) and Giorgia Fattorini (Università Politecnica delle Marche) for useful advices.

Footnotes

1. ^ The vesicular transporter described by McIntire et al. (1997) and Sagné et al. (1997) does transport both GABA and glycine (Wojcik et al., 2006), and is termed either VGAT (McIntire et al., 1997) or VIAAT (Sagné et al., 1997). Here, in line with its widespread usage and with the more prominent role of GABA in neocortex, we follow the terminology of McIntire et al. (1997).

References

Alonso-Nanclares, L., Minelli, A., Melone, M., Edwards, R. H., Defelipe, J., and Conti, F. (2004). Perisomatic glutamatergic axon terminals: a novel feature of cortical synaptology revealed by vesicular glutamate transporter 1 immunostaining. Neuroscience 123, 547–556. doi: 10.1016/j.neuroscience.2003.09.033

PubMed Abstract | CrossRef Full Text | Google Scholar

Atwood, H. L., and Karunanithi, S. (2002). Diversification of synaptic strength: presynaptic elements. Nat. Rev. Neurosci. 3, 497–516. doi: 10.1038/nrn876

PubMed Abstract | CrossRef Full Text | Google Scholar

Battaglia, G., Monn, J. A., and Schoepp, D. D. (1997). In vivo inhibition of veratridine-evoked release of striatal excitatory amino acids by the group II metabotropic glutamate receptor agonist LY354740 in rats. Neurosci. Lett. 229, 161–164. doi: 10.1016/S0304-3940(97)00442-4

PubMed Abstract | CrossRef Full Text | Google Scholar

Bellocchio, E. E., Hu, H., Pohorille, A., Chan, J., Pickel, V. M., and Edwards, R. H. (1998). The localization of the brain-specific inorganic phosphate transporter suggests a specific presynaptic role in glutamatergic transmission. J. Neurosci. 18, 8648–8659.

PubMed Abstract | Google Scholar

Bogen, I. L., Boulland, J. L., Mariussen, E., Wright, M. S., Fonnum, F., Kao, H. T., et al. (2006). Absence of synapsin I and II is accompanied by decreases in vesicular transport of specific neurotransmitters. J. Neurochem. 96, 1458–1466. doi: 10.1111/j.1471-4159.2005.03636.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Bolte, S., and Cordelieres, F. P. (2006). A guided tour into subcellular colocalization analysis in light microscopy. J. Microsc. 224, 213–232. doi: 10.1111/j.1365-2818.2006.01706.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Bonanno, G., Giambelli, R., Raiteri, L., Tiraboschi, E., Zappettini, S., Musazzi, L., et al. (2005). Chronic antidepressants reduce depolarization-evoked glutamate release and protein interactions favoring formation of SNARE complex in hippocampus. J. Neurosci. 25, 3270–3279. doi: 10.1523/JNEUROSCI.5033-04.2005

PubMed Abstract | CrossRef Full Text | Google Scholar

Bonanno, G., and Raiteri, M. (1993). Multiple GABAB receptors. Trends Pharmacol. Sci. 14, 259–261. doi: 10.1016/0165-6147(93)90124-3

CrossRef Full Text | Google Scholar

Bowery, N. G., Hill, D. R., Hudson, A. L., Doble, A., Middlemiss, D. N., Shaw, J., et al. (1980). Baclofen decreases neurotransmitter release in the mammalian CNS by an action at a novel GABA receptor. Nature 283, 92–94. doi: 10.1038/283092a0

PubMed Abstract | CrossRef Full Text | Google Scholar

Boyes, J., and Bolam, J. P. (2003). The subcellular localization of GABA (B) receptor subunits in the rat substantia nigra. Eur. J. Neurosci. 18, 3279–3293. doi: 10.1111/j.1460-9568.2003.03076.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Bradley, S. R., Levey, A. I., Hersch, S. M., and Conn, P. J. (1996). Immunocytochemical localization of group III metabotropic glutamate receptors in the hippocampus with subtype-specific antibodies. J. Neurosci. 16, 2044–2056.

PubMed Abstract | Google Scholar

Bragina, L., Candiracci, C., Barbaresi, P., Giovedi, S., Benfenati, F., and Conti, F. (2007). Heterogeneity of glutamatergic and GABAergic release machinery in cerebral cortex. Neuroscience 146, 1829–1840. doi: 10.1016/j.neuroscience.2007.02.060

PubMed Abstract | CrossRef Full Text | Google Scholar

Bragina, L., Fattorini, G., Giovedi, S., Melone, M., Bosco, F., Benfenati, F., et al. (2011). Analysis of synaptotagmin, SV2, and rab3 expression in cortical glutamatergic and GABAergic axon terminals. Front. Cell Neurosci. 5:32. doi: 10.3389/fncel.2011.00032

PubMed Abstract | CrossRef Full Text | Google Scholar

Bragina, L., Giovedi, S., Barbaresi, P., Benfenati, F., and Conti, F. (2010). Heterogeneity of glutamatergic and GABAergic release machinery in cerebral cortex: analysis of synaptogyrin, vesicle-associated membrane protein, and syntaxin. Neuroscience 165, 934–943. doi: 10.1016/j.neuroscience.2009.11.009

PubMed Abstract | CrossRef Full Text | Google Scholar

Bragina, L., Melone, M., Fattorini, G., Torres-Ramos, M., Vallejo-Illarramendi, A., Matute, C., et al. (2006). GLT-1 down-regulation induced by clozapine in rat frontal cortex is associated with synaptophysin up-regulation. J. Neurochem. 99, 134–141. doi: 10.1111/j.1471-4159.2006.04030.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Bruno, V., Battaglia, G., Kingston, A., O’neill, M. J., Catania, M. V., Di Grezia, R., et al. (1999). Neuroprotective activity of the potent and selective mGlu1a metabotropic glutamate receptor antagonist, (+)-2-methyl-4 carboxyphenylglycine (LY367385): comparison with LY357366, a broader spectrum antagonist with equal affinity for mGlu1a and mGlu5 receptors. Neuropharmacology 38, 199–207. doi: 10.1016/S0028-3908(98)00159-2

PubMed Abstract | CrossRef Full Text | Google Scholar

Chaudhry, F. A., Reimer, R. J., Bellocchio, E. E., Danbolt, N. C., Osen, K. K., Edwards, R. H., et al. (1998). The vesicular GABA transporter, VGAT, localizes to synaptic vesicles in sets of glycinergic as well as GABAergic neurons. J. Neurosci. 18, 9733–9750.

PubMed Abstract | Google Scholar

Chen, L., Boyes, J., Yung, W. H., and Bolam, J. P. (2004). Subcellular localization of GABAB receptor subunits in rat globus pallidus. J. Comp. Neurol. 474, 340–352. doi: 10.1002/cne.20143

PubMed Abstract | CrossRef Full Text | Google Scholar

Conti, F., Candiracci, C., and Fattorini, G. (2005). Heterogeneity of axon terminals expressing VGLUT1 in the cerebral neocortex. Arch. Ital. Biol. 143, 127–132.

PubMed Abstract | Google Scholar

Cubelos, B., Gimenez, C., and Zafra, F. (2005). Localization of the GLYT1 glycine transporter at glutamatergic synapses in the rat brain. Cereb. Cortex 15, 448–459. doi: 10.1093/cercor/bhh147

PubMed Abstract | CrossRef Full Text | Google Scholar

Dalezios, Y., Lujan, R., Shigemoto, R., Roberts, J. D., and Somogyi, P. (2002). Enrichment of mGluR7a in the presynaptic active zones of GABAergic and non-GABAergic terminals on interneurons in the rat somatosensory cortex. Cereb. Cortex 12, 961–974. doi: 10.1093/cercor/12.9.961

PubMed Abstract | CrossRef Full Text | Google Scholar

Donoghue, J. P., and Wise, S. P. (1982). The motor cortex of the rat: cytoarchitecture and microstimulation mapping. J. Comp. Neurol. 212, 76–88. doi: 10.1002/cne.902120106

PubMed Abstract | CrossRef Full Text | Google Scholar

Dunkley, P. R., Jarvie, P. E., Heath, J. W., Kidd, G. J., and Rostas, J. A. (1986). A rapid method for isolation of synaptosomes on Percoll gradients. Brain Res. 372, 115–129. doi: 10.1016/0006-8993(86)91464-2

CrossRef Full Text | Google Scholar

Froestl, W., Mickel, S. J., Von Sprecher, G., Diel, P. J., Hall, R. G., Maier, L., et al. (1995). Phosphinic acid analogues of GABA. 2. Selective, orally active GABAB antagonists. J. Med. Chem. 38, 3313–3331. doi: 10.1021/jm00017a016

CrossRef Full Text | Google Scholar

Gasparini, F., Lingenhohl, K., Stoehr, N., Flor, P. J., Heinrich, M., Vranesic, I., et al. (1999). 2-Methyl-6-(phenylethynyl)-pyridine (MPEP), a potent, selective and systemically active mGlu5 receptor antagonist. Neuropharmacology 38, 1493–1503. doi: 10.1016/S0028-3908(99)00082-9

CrossRef Full Text | Google Scholar

Gereau, R. W., and Swanson, G. T. (2008). The Receptors: The Glutamate Receptors. Totowa: Humana Press. doi: 10.1007/978-1-59745-055-3

CrossRef Full Text

Giribaldi, F., Milanese, M., Bonifacino, T., Anna Rossi, P. I., Di Prisco, S., Pittaluga, A., et al. (2013). Group I metabotropic glutamate autoreceptors induce abnormal glutamate exocytosis in a mouse model of amyotrophic lateral sclerosis. Neuropharmacology 66, 253–263. doi: 10.1016/j.neuropharm.2012.05.018

PubMed Abstract | CrossRef Full Text | Google Scholar

Gonchar, Y., Pang, L., Malitschek, B., Bettler, B., and Burkhalter, A. (2001). Subcellular localization of GABA(B) receptor subunits in rat visual cortex. J. Comp. Neurol. 431, 182–197. doi: 10.1002/1096-9861(20010305)431:2<182::AID-CNE1064>3.0.CO;2-K

PubMed Abstract | CrossRef Full Text | Google Scholar

Heja, L., Barabas, P., Nyitrai, G., Kekesi, K. A., Lasztoczi, B., Toke, O., et al. (2009). Glutamate uptake triggers transporter-mediated GABA release from astrocytes. PLoS ONE 4:e7153. doi: 10.1371/journal.pone.0007153

PubMed Abstract | CrossRef Full Text | Google Scholar

Herrero, I., Miras-Portugal, M. T., and Sanchez-Prieto, J. (1992). Positive feedback of glutamate exocytosis by metabotropic presynaptic receptor stimulation. Nature 360, 163–166. doi: 10.1038/360163a0

PubMed Abstract | CrossRef Full Text | Google Scholar

Kaneko, T., Fujiyama, F., and Hioki, H. (2002). Immunohistochemical localization of candidates for vesicular glutamate transporters in the rat brain. J. Comp. Neurol. 444, 39–62. doi: 10.1002/cne.10129

PubMed Abstract | CrossRef Full Text | Google Scholar

Kaupmann, K., Malitschek, B., Schuler, V., Heid, J., Froestl, W., Beck, P., et al. (1998). GABA(B)-receptor subtypes assemble into functional heteromeric complexes. Nature 396, 683–687. doi: 10.1038/25360

PubMed Abstract | CrossRef Full Text | Google Scholar

Kingston, A. E., Ornstein, P. L., Wright, R. A., Johnson, B. G., Mayne, N. G., Burnett, J. P., et al. (1998). LY341495 is a nanomolar potent and selective antagonist of group II metabotropic glutamate receptors. Neuropharmacology 37, 1–12. doi: 10.1016/S0028-3908(97)00191-3

CrossRef Full Text | Google Scholar

Kinoshita, A., Shigemoto, R., Ohishi, H., Van Der Putten, H., and Mizuno, N. (1998). Immunohistochemical localization of metabotropic glutamate receptors, mGluR7a and mGluR7b, in the central nervous system of the adult rat and mouse: a light and electron microscopic study. J. Comp. Neurol. 393, 332–352. doi: 10.1002/(SICI)1096-9861(19980413)393:3<332::AID-CNE6>3.0.CO;2-2

PubMed Abstract | CrossRef Full Text | Google Scholar

Kinzie, J. M., Shinohara, M. M., Van Den Pol, A. N., Westbrook, G. L., and Segerson, T. P. (1997). Immunolocalization of metabotropic glutamate receptor 7 in the rat olfactory bulb. J. Comp. Neurol. 385, 372–384. doi: 10.1002/(SICI)1096-9861(19970901)385:3<372::AID-CNE3>3.0.CO;2-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Kosinski, C. M., Risso Bradley, S., Conn, P. J., Levey, A. I., Landwehrmeyer, G. B., Penney, J. B., et al. (1999). Localization of metabotropic glutamate receptor 7 mRNA and mGluR7a protein in the rat basal ganglia. J. Comp. Neurol. 415, 266–284. doi: 10.1002/(SICI)1096-9861(19991213)415:2<266::AID-CNE9>3.0.CO;2-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Kulik, A., Nakadate, K., Nyiri, G., Notomi, T., Malitschek, B., Bettler, B., et al. (2002). Distinct localization of GABA(B) receptors relative to synaptic sites in the rat cerebellum and ventrobasal thalamus. Eur. J. Neurosci. 15, 291–307. doi: 10.1046/j.0953-816x.2001.01855.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Kulik, A., Vida, I., Lujan, R., Haas, C. A., Lopez-Bendito, G., Shigemoto, R., et al. (2003). Subcellular localization of metabotropic GABA(B) receptor subunits GABA(B1a/b) and GABA(B2) in the rat hippocampus. J. Neurosci. 23, 11026–11035.

PubMed Abstract | Google Scholar

Lacey, C. J., Boyes, J., Gerlach, O., Chen, L., Magill, P. J., and Bolam, J. P. (2005). GABA(B) receptors at glutamatergic synapses in the rat striatum. Neuroscience 136, 1083–1095. doi: 10.1016/j.neuroscience.2005.07.013

PubMed Abstract | CrossRef Full Text | Google Scholar

Lachmanovich, E., Shvartsman, D. E., Malka, Y., Botvin, C., Henis, Y. I., and Weiss, A. M. (2003). Co-localization analysis of complex formation among membrane proteins by computerized fluorescence microscopy: application to immunofluorescence co-patching studies. J. Microsc. 212, 122–131. doi: 10.1046/j.1365-2818.2003.01239.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, X., Gardner, E. L., and Xi, Z. X. (2008). The metabotropic glutamate receptor 7 (mGluR7) allosteric agonist AMN082 modulates nucleus accumbens GABA and glutamate, but not dopamine, in rats. Neuropharmacology 54, 542–551. doi: 10.1016/j.neuropharm.2007.11.005

PubMed Abstract | CrossRef Full Text | Google Scholar

Liu, X. B., Low, L. K., Jones, E. G., and Cheng, H. J. (2005). Stereotyped axon pruning via plexin signaling is associated with synaptic complex elimination in the hippocampus. J. Neurosci. 25, 9124–9134. doi: 10.1523/JNEUROSCI.2648-05.2005

PubMed Abstract | CrossRef Full Text | Google Scholar

Lopez-Bendito, G., Shigemoto, R., Kulik, A., Paulsen, O., Fairen, A., and Lujan, R. (2002). Expression and distribution of metabotropic GABA receptor subtypes GABABR1 and GABABR2 during rat neocortical development. Eur. J. Neurosci. 15, 1766–1778. doi: 10.1046/j.1460-9568.2002.02032.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Lujan, R., Roberts, J. D., Shigemoto, R., Ohishi, H., and Somogyi, P. (1997). Differential plasma membrane distribution of metabotropic glutamate receptors mGluR1 alpha, mGluR2 and mGluR5, relative to neurotransmitter release sites. J. Chem. Neuroanat. 13, 219–241. doi: 10.1016/S0891-0618(97)00051-3

PubMed Abstract | CrossRef Full Text | Google Scholar

Lujan, R., and Shigemoto, R. (2006). Localization of metabotropic GABA receptor subunits GABAB1 and GABAB2 relative to synaptic sites in the rat developing cerebellum. Eur. J. Neurosci. 23, 1479–1490. doi: 10.1111/j.1460-9568.2006.04669.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Lujan, R., Shigemoto, R., Kulik, A., and Juiz, J. M. (2004). Localization of the GABAB receptor 1a/b subunit relative to glutamatergic synapses in the dorsal cochlear nucleus of the rat. J. Comp. Neurol. 475, 36–46. doi: 10.1002/cne.20160

PubMed Abstract | CrossRef Full Text | Google Scholar

Lujan, R., Shigemoto, R., and Lopez-Bendito, G. (2005). Glutamate and GABA receptor signalling in the developing brain. Neuroscience 130, 567–580. doi: 10.1016/j.neuroscience.2004.09.042

PubMed Abstract | CrossRef Full Text | Google Scholar

McDonald, A. J., Mascagni, F., and Muller, J. F. (2004). Immunocytochemical localization of GABABR1 receptor subunits in the basolateral amygdala. Brain Res. 1018, 147–158. doi: 10.1016/j.brainres.2004.05.053

PubMed Abstract | CrossRef Full Text | Google Scholar

McIntire, S. L., Reimer, R. J., Schuske, K., Edwards, R. H., and Jorgensen, E. M. (1997). Identification and characterization of the vesicular GABA transporter. Nature 389, 870–876. doi: 10.1038/39908

PubMed Abstract | CrossRef Full Text | Google Scholar

Melone, M., Burette, A., and Weinberg, R. J. (2005). Light microscopic identification and immunocytochemical characterization of glutamatergic synapses in brain sections. J. Comp. Neurol. 492, 495–509. doi: 10.1002/cne.20743

PubMed Abstract | CrossRef Full Text | Google Scholar

Minelli, A., Alonso-Nanclares, L., Edwards, R. H., Defelipe, J., and Conti, F. (2003). Postnatal development of the vesicular GABA transporter in rat cerebral cortex. Neuroscience 117, 337–346. doi: 10.1016/S0306-4522(02)00864-3

CrossRef Full Text | Google Scholar

Mitsukawa, K., Yamamoto, R., Ofner, S., Nozulak, J., Pescott, O., Lukic, S., et al. (2005). A selective metabotropic glutamate receptor 7 agonist: activation of receptor signaling via an allosteric site modulates stress parameters in vivo. Proc. Natl. Acad. Sci. U.S.A. 102, 18712–18717. doi: 10.1073/pnas.0508063102

PubMed Abstract | CrossRef Full Text | Google Scholar

Monn, J. A., Valli, M. J., Massey, S. M., Hansen, M. M., Kress, T. J., Wepsiec, J. P., et al. (1999). Synthesis, pharmacological characterization, and molecular modeling of heterobicyclic amino acids related to (+)-2-aminobicyclo[3.1.0] hexane-2,6-dicarboxylic acid (LY354740): identification of two new potent, selective, and systemically active agonists for group II metabotropic glutamate receptors. J. Med. Chem. 42, 1027–1040. doi: 10.1021/jm980616n

PubMed Abstract | CrossRef Full Text | Google Scholar

Muly, E. C., Maddox, M., and Smith, Y. (2003). Distribution of mGluR1alpha and mGluR5 immunolabeling in primate prefrontal cortex. J. Comp. Neurol. 467, 521–535. doi: 10.1002/cne.10937

PubMed Abstract | CrossRef Full Text | Google Scholar

Musante, V., Neri, E., Feligioni, M., Puliti, A., Pedrazzi, M., Conti, V., et al. (2008). Presynaptic mGlu1 and mGlu5 autoreceptors facilitate glutamate exocytosis from mouse cortical nerve endings. Neuropharmacology 55, 474–482. doi: 10.1016/j.neuropharm.2008.06.056

PubMed Abstract | CrossRef Full Text | Google Scholar

Neki, A., Ohishi, H., Kaneko, T., Shigemoto, R., Nakanishi, S., and Mizuno, N. (1996). Pre- and postsynaptic localization of a metabotropic glutamate receptor, mGluR2, in the rat brain: an immunohistochemical study with a monoclonal antibody. Neurosci. Lett. 202, 197–200. doi: 10.1016/0304-3940(95)12248-6

PubMed Abstract | CrossRef Full Text | Google Scholar

Ohishi, H., Ogawa-Meguro, R., Shigemoto, R., Kaneko, T., Nakanishi, S., and Mizuno, N. (1994). Immunohistochemical localization of metabotropic glutamate receptors, mGluR2 and mGluR3, in rat cerebellar cortex. Neuron 13, 55–66. doi: 10.1016/0896-6273(94)90459-6

PubMed Abstract | CrossRef Full Text | Google Scholar

Paquet, M., and Smith, Y. (2003). Group I metabotropic glutamate receptors in the monkey striatum: subsynaptic association with glutamatergic and dopaminergic afferents. J. Neurosci. 23, 7659–7669.

PubMed Abstract | Google Scholar

Petralia, R. S., Wang, Y. X., Niedzielski, A. S., and Wenthold, R. J. (1996). The metabotropic glutamate receptors, mGluR2 and mGluR3, show unique postsynaptic, presynaptic and glial localizations. Neuroscience 71, 949–976. doi: 10.1016/0306-4522(95)00533-1

CrossRef Full Text | Google Scholar

Pin, J. P., and Duvoisin, R. (1995). The metabotropic glutamate receptors: structure and functions. Neuropharmacology 34, 1–26. doi: 10.1016/0028-3908(94)00129-G

CrossRef Full Text | Google Scholar

Pinheiro, P. S., and Mulle, C. (2008). Presynaptic glutamate receptors: physiological functions and mechanisms of action. Nat. Rev. Neurosci. 9, 423–436. doi: 10.1038/nrn2379

PubMed Abstract | CrossRef Full Text | Google Scholar

Pinheiro, P. S., Perrais, D., Coussen, F., Barhanin, J., Bettler, B., Mann, J. R., et al. (2007). GluR7 is an essential subunit of presynaptic kainate autoreceptors at hippocampal mossy fiber synapses. Proc. Natl. Acad. Sci. U.S.A. 104, 12181–12186. doi: 10.1073/pnas.0608891104

PubMed Abstract | CrossRef Full Text | Google Scholar

Raiteri, M. (2008). “Presynaptic metabotropic glutamate and GABAB receptors,” in Pharmacology of Neurotrasmitter Release. Handbook of Experimental Pharmacology, eds T. C. Sudof and K. Starke (Berlin: Springer), 374–400.

Raiteri, M., Bonanno, G., Marchi, M., and Maura, G. (1984). Is there a functional linkage between neurotransmitter uptake mechanisms and presynaptic receptors? J. Pharmacol. Exp. Ther. 231, 671–677.

PubMed Abstract | Google Scholar

Reid, S. N., Romano, C., Hughes, T., and Daw, N. W. (1995). Immunohistochemical study of two phosphoinositide-linked metabotropic glutamate receptors (mGluR1 alpha and mGluR5) in the cat visual cortex before, during, and after the peak of the critical period for eye-specific connections. J. Comp. Neurol. 355, 470–477. doi: 10.1002/cne.903550311

PubMed Abstract | CrossRef Full Text | Google Scholar

Rodrigues, R. J., Alfaro, T. M., Rebola, N., Oliveira, C. R., and Cunha, R. A. (2005). Co-localization and functional interaction between adenosine A(2A) and metabotropic group 5 receptors in glutamatergic nerve terminals of the rat striatum. J. Neurochem. 92, 433–441. doi: 10.1111/j.1471-4159.2004.02887.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Romano, C., Sesma, M. A., Mcdonald, C. T., O’malley, K., Van Den Pol, A. N., and Olney, J. W. (1995). Distribution of metabotropic glutamate receptor mGluR5 immunoreactivity in rat brain. J. Comp. Neurol. 355, 455–469. doi: 10.1002/cne.903550310

PubMed Abstract | CrossRef Full Text | Google Scholar

Sagné, C., El Mestikawy, S., Isambert, M. F., Hamon, M., Henry, J. P., Giros, B., et al. (1997). Cloning of a functional vesicular GABA and glycine transporter by screening of genome database. FEBS Lett. 417, 177–183. doi: 10.1016/S0014-5793(97)01279-9

PubMed Abstract | CrossRef Full Text | Google Scholar

Shigemoto, R., Kinoshita, A., Wada, E., Nomura, S., Ohishi, H., Takada, M., et al. (1997). Differential presynaptic localization of metabotropic glutamate receptor subtypes in the rat hippocampus. J. Neurosci. 17, 7503–7522.

PubMed Abstract | Google Scholar

Shigemoto, R., Kulik, A., Roberts, J. D., Ohishi, H., Nusser, Z., Kaneko, T., et al. (1996). Target-cell-specific concentration of a metabotropic glutamate receptor in the presynaptic active zone. Nature 381, 523–525. doi: 10.1038/381523a0

PubMed Abstract | CrossRef Full Text | Google Scholar

Somogyi, P., Dalezios, Y., Lujan, R., Roberts, J. D., Watanabe, M., and Shigemoto, R. (2003). High level of mGluR7 in the presynaptic active zones of select populations of GABAergic terminals innervating interneurons in the rat hippocampus. Eur. J. Neurosci. 17, 2503–2520. doi: 10.1046/j.1460-9568.2003.02697.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Staple, J. K., Morgenthaler, F., and Catsicas, S. (2000). Presynaptic heterogeneity: vive la difference. News Physiol. Sci. 15, 45–49.

PubMed Abstract | Google Scholar

Stigliani, S., Zappettini, S., Raiteri, L., Passalacqua, M., Melloni, E., Venturi, C., et al. (2006). Glia re-sealed particles freshly prepared from adult rat brain are competent for exocytotic release of glutamate. J. Neurochem. 96, 656–668. doi: 10.1111/j.1471-4159.2005.03631.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Sugino, K., Hempel, C. M., Miller, M. N., Hattox, A. M., Shapiro, P., Wu, C., et al. (2006). Molecular taxonomy of major neuronal classes in the adult mouse forebrain. Nat. Neurosci. 9, 99–107. doi: 10.1038/nn1618

PubMed Abstract | CrossRef Full Text | Google Scholar

Summa, M., Di Prisco, S., Grilli, M., Usai, C., Marchi, M., and Pittaluga, A. (2013). Presynaptic mGlu7 receptors control GABA release in mouse hippocampus. Neuropharmacology 66, 215–224. doi: 10.1016/j.neuropharm.2012.04.020

PubMed Abstract | CrossRef Full Text | Google Scholar

Suzuki, G., Tsukamoto, N., Fushiki, H., Kawagishi, A., Nakamura, M., Kurihara, H., et al. (2007). In vitro pharmacological characterization of novel isoxazolopyridone derivatives as allosteric metabotropic glutamate receptor 7 antagonists. J. Pharmacol. Exp. Ther. 323, 147–156. doi: 10.1124/jpet.107.124701

PubMed Abstract | CrossRef Full Text | Google Scholar

Tabata, T., Araishi, K., Hashimoto, K., Hashimotodani, Y., Van Der Putten, H., Bettler, B., et al. (2004). Ca2⁺ activity at GABAB receptors constitutively promotes metabotropic glutamate signaling in the absence of GABA. Proc. Natl. Acad. Sci. U.S.A. 101, 16952–16957. doi: 10.1073/pnas.0405387101

PubMed Abstract | CrossRef Full Text | Google Scholar

Tafoya, L. C., Mameli, M., Miyashita, T., Guzowski, J. F., Valenzuela, C. F., and Wilson, M. C. (2006). Expression and function of SNAP-25 as a universal SNARE component in GABAergic neurons. J. Neurosci. 26, 7826–7838. doi: 10.1523/JNEUROSCI.1866-06.2006

PubMed Abstract | CrossRef Full Text | Google Scholar

Tamaru, Y., Nomura, S., Mizuno, N., and Shigemoto, R. (2001). Distribution of metabotropic glutamate receptor mGluR3 in the mouse CNS: differential location relative to pre- and postsynaptic sites. Neuroscience 106, 481–503. doi: 10.1016/S0306-4522(01)00305-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Varoqui, H., Schafer, M. K., Zhu, H., Weihe, E., and Erickson, J. D. (2002). Identification of the differentiation-associated Na⁺/PI transporter as a novel vesicular glutamate transporter expressed in a distinct set of glutamatergic synapses. J. Neurosci. 22, 142–155.

Google Scholar

Welker, C. (1971). Microelectrode delineation of fine grain somatotopic organization of (SmI) cerebral neocortex in albino rat. Brain Res. 26, 259–275. doi: 10.1016/s0006-8993(71)80004-5

PubMed Abstract | CrossRef Full Text | Google Scholar

White, J. H., Wise, A., Main, M. J., Green, A., Fraser, N. J., Disney, G. H., et al. (1998). Heterodimerization is required for the formation of a functional GABA (B) receptor. Nature 396, 679–682. doi: 10.1038/25354

PubMed Abstract | CrossRef Full Text | Google Scholar

Wisniewski, K., and Car, H. (2002). (S)-3,5-DHPG: a review. CNS Drug Rev. 8, 101–116. doi: 10.1111/j.1527-3458.2002.tb00218.x

CrossRef Full Text | Google Scholar

Wojcik, S. M., Katsurabayashi, S., Guillemin, I., Friauf, E., Rosenmund, C., Brose, N., et al. (2006). A shared vesicular carrier allows synaptic corelease of GABA and glycine. Neuron 50, 575–587. doi: 10.1016/j.neuron.2006.04.016

PubMed Abstract | CrossRef Full Text | Google Scholar

Woolsey, C. N., and LeMessurier, D. H. (1948). The pattern of cutaneous representation in the rat’s cerebral cortex. Fed. Proc. 7(Pt 1), 137.

Google Scholar

Zilles, K., Zilles, B., and Schleicher, A. (1980). A quantitative approach to cytoarchitectonics. VI. The areal pattern of the cortex of the albino rat. Anat. Embryol. 159, 335–360. doi: 10.1007/BF00317655

PubMed Abstract | CrossRef Full Text | Google Scholar