AUTHOR=Dogbevia Godwin K. , Marticorena-Alvarez Ricardo , Bausen Melanie , Sprengel Rolf , Hasan Mazahir T. 

TITLE=Inducible and combinatorial gene manipulation in mouse brain

JOURNAL=Frontiers in Cellular Neuroscience

VOLUME=9

YEAR=2015

URL=https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2015.00142

DOI=10.3389/fncel.2015.00142

ISSN=1662-5102

ABSTRACT=<p>We have deployed recombinant adeno-associated viruses equipped with tetracycline-controlled genetic switches to manipulate gene expression in mouse brain. Here, we show a combinatorial genetic approach for inducible, cell type-specific gene expression and Cre/loxP mediated gene recombination in different brain regions. Our chemical-genetic approach will help to investigate ‘when’, ‘where’, and ‘how’ gene(s) control neuronal circuit dynamics, and organize, for example, sensory signal processing, learning and memory, and behavior.</p>