Epigenetic regulation in the inner ear and its potential roles in development, protection, and regeneration

The burgeoning field of epigenetics is beginning to make a significant impact on our understanding of tissue development, maintenance, and function. Epigenetic mechanisms regulate the structure and activity of the genome in response to intracellular and environmental cues that direct cell-type specific gene networks. The inner ear is comprised of highly specialized cell types with identical genomes that originate from a single totipotent zygote. During inner ear development specific combinations of transcription factors and epigenetic modifiers must function in a coordinated manner to establish and maintain cellular identity. These epigenetic regulatory mechanisms contribute to the maintenance of distinct chromatin states and cell-type specific gene expression patterns. In this review, we highlight emerging paradigms for epigenetic modifications related to inner ear development, and how epigenetics may have a significant role in hearing loss, protection, and regeneration.

Introduction

In 1942, C. H. Waddington coined the term epigenetics by combining the words epigenesis and genetics (Waddington, 2012). Epigenesis refers to the sequence of events that occur during differentiation of cells from their initial totipotent state to a fully developed multicellular organism. The physical nature of genes and their role in heredity was unknown at the time Waddington coined this term. However, he used the term epigenetics to refer to the increasing irreversibility of cellular differentiation as a cell becomes more differentiated during development. The field of developmental biology continues striving to better understand how a single totipotent cell containing one genome has the ability to generate millions of highly specialized fully differentiated cells with very different gene regulatory networks.

The definition of epigenetics has evolved over the years and continues to be debated by scientists. Epigenetics was classically defined as heritable changes in gene function that cannot be explained by changes in DNA sequence and typically referred to DNA methylation related to parental genomic imprinting. However, this definition has changed drastically in recent years to encompass non-heritable changes that alter gene function including dynamic chromatin states regulated by various histone modifications and chromatin remodeling proteins. The development of the “omics era” has greatly impacted the way scientists now view epigenetics since many human developmental disorders and cancer have been correlated with the misregulation of specific epigenetic events and has driven the development of epigenetic therapeutics.

Although a vast amount of data about epigenetics exists for other tissues, there is a noticeable lack of information about epigenetic modifications in the inner ear. Proper epigenetic modifications are required for normal developmental processes. Gene expression must be coordinated in a temporal and cell-type specific manner and requires multiple levels of gene regulation. Although transcription factors are a primary source of gene regulation, epigenetic modifications regulate transcription factor access to target genes. This concept is apparent from studies looking at direct cellular reprogramming through ectopic expression of defined transcription factors, which show that direct reprogramming is a slow and inefficient process with most cells failing to reprogram (Huangfu et al., 2008; Mikkelsen et al., 2008). In the auditory field, ectopic expression of transcription factors such as Atoh1 has been used to convert mammalian non-sensory epithelial cells into cells that express many endogenous hair cell markers (Zheng and Gao, 2000; Izumikawa et al., 2005; Gubbels et al., 2008). However, the reprogramming process of transforming supporting cells into hair cells may not be solely about genetic transformation, but also epigenetic transformation. Studies using induced pluripotent stem cells (iPSCs) have shown that they retain the epigenetic memory of their somatic cell of origin (Kim et al., 2010; Lister et al., 2011). The epigenetic memory retained by iPSCs can interfere with their potential for differentiation into other cell types (Li et al., 2009; Kim et al., 2010; Lister et al., 2011). Additionally, iPSCs derived from aged mice have a decreased potential for reprogramming compared to iPSCs derived from juvenile mice (Li et al., 2009; Lister et al., 2011). Although ectopic expression of transcription factors (Atoh1) can convert neonatal non-sensory epithelial cells into hair cell-like cells, loss of cellular plasticity at later postnatal ages could largely impact clinical application of this method (Kelly et al., 2012; Liu et al., 2012b).

In this review, we discuss the different types of epigenetic modifications and regulatory mechanisms in regards to development, disease, protection, and cellular reprogramming.

Histone Modifications

Nucleosomes form the fundamental repeating units of eukaryotic chromatin, which is used to package large eukaryotic genomes into the nucleus while still ensuring appropriate access to the chromatin (Kornberg, 1974; Kornberg and Thomas, 1974). Nucleosomes are folded through a series of successively higher order structures to both compact DNA and create an added layer of regulatory control ensuring correct gene expression. The nucleosome is comprised of approximately 147 base pairs of DNA wrapped around eight histone core subunits consisting of two copies each of the core histones H2A, H2B, H3, and H4 (Luger et al., 1997). Histone H3 and H4 have long tails that protrude from the nucleosome and can be covalently modified at several sites (Figure 1; Vaquero et al., 2003; Campos and Reinberg, 2009; Bannister and Kouzarides, 2011). These histone tails are subjected to post-translational modification including acetylation, methylation, phosphorylation, ubiquitination, sumoylation, or ADP-ribosylation which is mediated by the counteracting activities of enzymes that add or remove such modifications (Vaquero et al., 2003; Campos and Reinberg, 2009; Bannister and Kouzarides, 2011).

FIGURE 1

Figure 1. Cartoon diagram indicates potential sites of modification at specific residues along the histones tail. The tails of histone H3 and H4 have the largest number of potential modification sites including lysine (K)-specific methylation and acetylation sites and arginine (R)-specific methylation sites. The tail of histone H3 is subject to both repressive lysine (K)-specific methylation marks (K9 and K27) as well as activating lysine (K)-specific methylation marks (K4, K36, and K79).

Histone Acetylation

Posttranslational histone modifications alter histones interaction with DNA and nuclear proteins. Histone acetylation of specific lysine residues plays a fundamental role in transcriptional regulation. The enzymes responsible for maintaining proper histone acetylation states include histone acetyltransferases (HATs) and histone deacetylases (HDACs). Histones undergo acetylation to yield a more relaxed chromatin conformation resulting from a net change in the overall charge and reduced electrostatic interactions. HATs transfer the acetyl moiety from acetyl coenzyme A to specific lysine residues on the histone tail. Acetylated histones also act as a signal that recruits bromodomain-containing proteins, which are primarily transcription factors and cofactors to target genes activating their transcription (Zeng and Zhou, 2002). The HDACs act in opposition to the HATs by removing the acetyl groups from histone tails allowing histones to interact with DNA more tightly to form a compacted nucleosome structure. This increased rigidity of the chromatin prevents the incorporation of transcriptional machinery, effectively silencing gene transcription (Figure 2).

FIGURE 2

Figure 2. Chromatin state. Cartoon diagram depicting the open relaxed chromatin of an actively transcribed gene (upper portion), compared to the nucleosome dense compacted chromatin associated with a silenced gene (bottom portion). HMT—histone methyltransferase, HAT—histone acetyltransferase, TBP—TATA-binding protein, TAF—TBP-associated factors, TF—transcription factor, HDAC—histone deacetylase, KDM—lysine (K)-specific demethylase, DNMT—DNA methyltransferase, MBD—methyl-CpG-binding domain.

Histone Methylation and Demethylation

Histone methylation, depending on both the histone and residue modified, contributes to either active or repressive chromatin configurations (Figure 2). Although histone methylation is not as well understood as acetylation, histones H3 and H4 are common methylation targets that can be methylated on arginine and lysine residues (Figure 1). While lysine can receive only one acetyl group, it can receive up to three methyl groups, and does not affect the overall charge of the residue. Specific histone methylation states serve as binding sites for the recruitment of additional regulatory proteins such as chromatin remodelers (Zhang and Dent, 2005; Wu and Zhang, 2009; Helin and Dhanak, 2013). As a general rule of thumb, sites of methylation that are typically associated with active gene transcription are H3K4me2/3 (promoter), H3K36me3 (3′ end gene body), and H3K79me2 (5′ end of gene body) (Azuara et al., 2006; Kolasinska-Zwierz et al., 2009; Onder et al., 2012; Fuchs et al., 2014). Whereas methylation marks associated typically with silenced genes are H3K9me2/3 (promoter and enhancer), H3K27me3 (promoter and enhancer), and H4K20me3 (promoter) (Azuara et al., 2006; Kolasinska-Zwierz et al., 2009). These common active and repressive histone marks are further illustrated in relation to the gene region in which they are detected in Figure 3.

FIGURE 3

Figure 3. Chromatin modifications are distributed in specific gene regulatory regions. (A) The normal distribution of DNA methylation, DNA hydroxymethylation, and histone marks in the enhancer, promoter, and gene body of actively transcribed genes. Actively transcribed genes carry typically have chromatin modifications within the gene body to facilitate transcription initiation and elongation. (B) Common chromatin modifications found in the enhancer, promoter, and gene body of silenced genes. (C) Bivalent/poised genes have both activating and silencing chromatin modifications to facilitate rapid changes in gene expression during development.

Studies of histone methylation marks in mouse pluripotent embryonic stem cells (ESCs) have defined a class of developmentally regulated genes as “bivalent” since these genes are marked with both active (H3K4me3) and repressive (H3K27me3) histone modifications (Figure 3; Azuara et al., 2006; Bernstein et al., 2006; Mikkelsen et al., 2007; Voigt et al., 2013). By exhibiting both active and repressive features, bivalent genes are posited as being in a poised state, enabling them to be rapidly activated upon suitable developmental cues and/or environmental stimuli. Although bivalent genes were first described for ESCs, where the marks are most prevalent, later observations also detected bivalent domains in cell types of restricted potency such as neural progenitor cells (Mikkelsen et al., 2007; Rugg-Gunn et al., 2010).

The enzymes responsible for maintaining proper histone methylation status are histone lysine/arginine methyltransferases and histone lysine/arginine demethylases. Lysine-specific histone methyltransferases (HMTs) are subdivided into SET (Su(var)3–9, Enhancer of Zeste, Trithorax) domain-containing and non-SET domain-containing proteins. The argine-specific protein arginine methyltransferases (PRMTs) are responsible for methylating arginine residues on the histones. HMTs and PRMTs together have over 60 different family members all of which use S-Adenosyl methionine (SAM) as a cofactor and methyl donor group (Helin and Dhanak, 2013).

Histone methylation for many years was considered to be a permanent and irreversible histone modification due to the low turnover rate of methylated histones (Byvoet et al., 1972). However, the discovery of lysine specific demethylase 1 (LSD1, officially known as KDM1A) and later the JmjC-domain-containing lysine demethylase family has completely changed this view (Kooistra and Helin, 2012). Interestingly, LSD1 can catalyze the demethylation of H3K4me1/2 and H3K9me1/2, which means that LSD1 has the ability to both silence and activate gene transcription (Zhang and Dent, 2005; Wu and Zhang, 2009; Helin and Dhanak, 2013). LSD1 is also reported to demethylate non-histone target proteins such as p53, DNMT1, and E2F1 (Huang et al., 2007; Wang et al., 2009; Kontaki and Talianidis, 2010; Helin and Dhanak, 2013; Mosammaparast et al., 2013). LSD1 and its close relative LSD2 belong to the superfamily of flavin adenine dinucleotide (FAD)-dependent monooxidases. Unlike the LSD demethylases, the JmjC-domain-containing demethylases can also demethylate trimethylated lysines. This catalysis involves an oxidative mechanism requiring iron and 2-oxoglutarate as co-factors and is posited to occur through direct hydroxylation of the affected methyl group.

Histone Variants

Although histones have a conserved role as general DNA packaging agents, it has become clear that another key function of these proteins is to confer variation in chromatin structure to ensure dynamic patterns of transcriptional regulation. Some histone variants have distinct biophysical characteristics that are thought to alter the properties of nucleosomes, while other variants are targeted to specific regions of the genome. Specific histone variants are exchanged with the pre-existing histones during critical periods of development and differentiation (Brandt et al., 1979; Grove and Zweidler, 1984; Wunsch et al., 1991; Bosch and Suau, 1995). This replacement can often result in the variants becoming the predominant species in the differentiated cell (Pina and Suau, 1987; Wunsch et al., 1991). Mutations in specific histone variants and their associated chaperone machinery contribute to human disease such as cancer (Maze et al., 2014), suggesting an essential function for regulation of histone variants during specific aspects of cellular differentiation.

DNA Methylation

Methylation at the 5-positon of cytosine (5-mC) is one of the best studied and most mechanistically understood epigenetic modifications that is well conserved among most plant, animal and fungal models (Feng et al., 2010). Three conserved DNA methyltransferase enzymes, DNA methyltransferase 1 (DNMT1), DNMT3A and DNMT3B, are responsible for the deposition and maintenance of DNA methylation and are essential for normal development (Li et al., 1992; Okano et al., 1999). Mammalian genomes are globally CpG-depleted and roughly 60–80% of the 28 million CpGs in the human genome are generally methylated (Smith and Meissner, 2013). Less than 10% of CpGs occur in the CG-dense regions called CpG islands (Deaton and Bird, 2011). CpG islands are prevalent at transcription start sites (TSSs) of housekeeping genes and genes involved in developmental processes (Deaton and Bird, 2011). Most genomic DNA methylation patterns are static across tissues and throughout life and only change in localized contexts as specific cellular processes are activated or repressed (Figure 3). However, the exception to this is in the germ line and during pre-implantation embryonic development, when DNA methylation levels are globally reset (Smith and Meissner, 2013).

Numerous assays have been developed since DNA methylation was originally postulated as an epigenetic regulator to study cytosine methylation (Holliday and Pugh, 1975; Riggs, 1975). This includes assays such as methylation-sensitive restriction enzyme mapping, deamination of unmethylated cytosines with sodium bisulphite, and targeting methylated DNA directly using antibodies for enrichment. High-throughput sequencing has enabled complete methylomes to be elucidated, such that methylation sites are now mapped at base-pair resolution across development from zygote to terminally differentiated adult cells. However, global DNA methylation patterns during development and aging are tissue and cell type-specific (Calvanese et al., 2012).

DNMT1 is critical for maintaining DNA methylation during mitosis. During DNA replication, DNMT1 is positioned at the replication fork and transfers the methylation marks to the newly synthesized daughter strand and is essential for stable repression of genes after cell division. Although DNA methylation maintenance ensures epigenetic inheritance at established positions, there are many instances in which methylation must be specifically targeted and others in which methylation must be inhibited or removed. DNMT3A and DNMT3B are responsible for establishing de novo DNA methylation, primarily at CpG dinucleotides (Jurkowska et al., 2011). DNMT3A and DNMT3B target promoters in complex with other epigenetic repressors, including HDACs and repressive HMTs such as EZH2 and G9a. Additionally, crosstalk exists between some site-specific transcription factors and DNMTs for example DNMT1, DNMT3A, and DNMT3B have been shown to interact with transcription factors such as E2F1, E2F6, and Atoh1 to facilitate or prevent DNA methylation at specific target genes (Robertson et al., 2000; Bossuyt et al., 2009; Velasco et al., 2010). Loss of all three DNMTs in ESCs does not affect their survival or stem cell molecular identity, but the ability to differentiate is completely inhibited (Jackson et al., 2004; Tsumura et al., 2006). ESCs lacking DNA methylation fail to up-regulate germ layer associated markers and are unable to efficiently silence pluripotency genes (Jackson et al., 2004). Typically the transcriptional network associated with pluripotency is rapidly silenced upon differentiation through both maintenance and de novo methylation, since embryonic programs must be resolved towards specific cell lineages.

5-mC was initially the only known DNA-specific epigenetic mark, then in 2009, 5-hydroxymethylcytosine (5-hmC) was discovered as another relatively abundant cytosine modification in mouse Purkinje neurons and ESCs (Kriaucionis and Heintz, 2009; Tahiliani et al., 2009). The ten-eleven translocation (TET) proteins mediate the oxidation of 5-mC to 5-hmC (Tahiliani et al., 2009; Wang et al., 2012), which is then further oxidized in a stepwise manner to 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC; He et al., 2011; Ito et al., 2011). An emerging finding is that 5-mC and 5-hmC are dynamically regulated both within and across cell types (Kriaucionis and Heintz, 2009; Tahiliani et al., 2009; Szulwach et al., 2011; Shen and Zhang, 2013). Although 5-hmC may simply act as a DNA demethylation intermediate, studies have shown that 5-hmC not only impairs the binding of 5-mC binding proteins (Valinluck et al., 2004), but also has its own unique binding protein, MBD3, (Yildirim et al., 2011) and shows unique distribution patterns in the genome (Stroud et al., 2011; Szulwach et al., 2011). 5-hmC is enriched in gene dense euchromatic regions, and particularly at TSSs, promoters, and enhancers (Shen and Zhang, 2013). Additionally, 5-hmC is specifically enriched at gene promoters associated with bivalent domains marked with both the permissive mark H3K4me2/3 and the repressive mark H3K27me3, but is absent from heterochromatin marked by H3K9me3 (Shen and Zhang, 2013).

Recent genome-wide analysis of DNA methylation in human cells has identified a widespread distribution of 5-mC and, paradoxically, has shown hypermethylation in the gene bodies of actively transcribed genes (Figure 3; Lister et al., 2009; Stadler et al., 2011; Hon et al., 2013; Ziller et al., 2013) and hypomethylation was found at active enhancers (Lister et al., 2009; Stadler et al., 2011; Hon et al., 2013; Ziller et al., 2013). 5-hmC is also significantly enriched at distal cis-regulatory sequences, suggesting that dynamic DNA methylation at these regions is likely mediated by interplays between DNMT mediated methylation and TET mediated demethylation processes (Stroud et al., 2011; Szulwach et al., 2011; Yu et al., 2012). Together, these studies have underscored the diverse roles that DNA methylation has in gene regulation and the need for systematic mapping and characterization of DNA methylomes in different tissues and cell types during development and aging. Since proper maintenance of 5-mC and 5-hmC by DNMT and TET proteins has been shown to be critical for proper neurodevelopment and memory (Wang et al., 2012), aberrant alterations in DNA methylation are also correlated with diseases such as diabetes, schizophrenia, multiple sclerosis, cancer, and cellular senescence (Jurkowska et al., 2011).

Chromatin Remodelers

At least three processes control the assembly and regulation of chromatin: histone modifications, DNA methylation, and ATP-dependent chromatin remodeling. ATP-dependent chromatin remodelers alter the physical state of chromatin by either sliding nucleosomes in relation to the DNA or exchanging nucleosomes into and out of DNA. Chromatin remodelers act as “readers” of the histone modifications to regulate chromatin structure and gene expression. Approximately 30 genes encode the ATP-dependent chromatin remodeling subunits in mammals. With few exceptions, the ATP-dependent chromatin remodeling proteins appear to be genetically non-redundant.

Mutations in ATP-dependent chromatin remodeling genes often have severe effects on the early embryo or give rise to maternal-effect phenotypes in which the phenotype of the embryo reflects the genotype of the mother. In many cases, the genes encoding the ATP-dependent chromatin remodeling proteins or their subunits are haploinsufficient, which indicates that their role in specific developmental processes is likely rate limiting. For instance, heterozygous mutation in the chromodomain helicase DNA binding protein 7 (CHD7) causes CHARGE syndrome, a multiple anomaly disorder that is a common cause of deaf-blindness in humans (Zentner et al., 2010), whereas duplication or overexpression of CHD7 is associated with multiple forms of cancer including colorectal cancer, pancreatic cancer, small-cell lung cancer, and gastric cancer (Pleasance et al., 2010; Kim et al., 2011; Colbert et al., 2014; Tahara et al., 2014). These data together suggest that many cell types may be highly sensitive to chromatin remodeler dosage during development and aging.

Epigenetics and Hearing Loss

Hereditary hearing loss or deafness has been associated with mutations in genes whose proteins regulate the chromatin state and include genes involved in DNA methylation, histone modification and chromatin remodeling (Table 1). Autosomal dominant cerebellar ataxia, deafness, and narcolepsy (ADCADN) and hereditary sensory neuropathy type IE (HSN1E) are both caused by heterozygous mutation in the DNMT1 gene (Sun et al., 2014). ADCADN is characterized by late onset (age 30–40 years) narcolepsy–cataplexy, sensorineural deafness, cerebellar ataxia, dementia, psychosis, optic atrophy, and other symptoms (Winkelmann et al., 2012). Narcolepsy and deafness are typically the first symptoms to appear followed by ataxia (Winkelmann et al., 2012). People with HSN1E develop hearing loss that is caused by abnormalities in the inner ear leading to sensorineural hearing loss (Wright and Dyck, 1995; Hojo et al., 1999; Klein et al., 2011). Hearing loss worsens over time and usually progresses to moderate or severe deafness between the ages of 20 and 35 (Wright and Dyck, 1995; Hojo et al., 1999; Klein et al., 2011). Mutations in DNMT1 typically cause bilateral hearing loss but unilateral hearing loss has also been reported (Melberg et al., 1995).

TABLE 1

Table 1. Epigenetic factors associated with hearing loss in humans.

Mutations in HMT have also been associated with hearing loss. Overgrowth disorders, Sotos syndrome and Weaver syndrome, are caused by heterozygous mutations in the HMT NSD1 (Sotos and Weaver syndromes) and EZH2 (Weaver syndrome) (Tatton-Brown and Rahman, 2013; Tatton-Brown et al., 2013). Sotos syndrome is frequently associated with conductive hearing loss while only a few cases of hearing loss have been reported for Weaver syndrome (Tatton-Brown et al., 2013). Although a large degree of phenotypic overlap exists between Sotos syndrome and Weaver syndrome, NSD1 and EZH2 regulate different methylation sites. NSD1 has been shown to preferentially dimethylate H3K36 and H4K20, the impact of dimethylation at either of these two lysine residues is not entirely clear at this time (Qiao et al., 2011). However, EZH2 has been well characterized as it functions as the catalytic subunit for the polycomb repressive complex 2 (PRC2) and is a critical regulator of H3K27me3.

Kleefstra syndrome is a developmental disorder characterized by intellectual disability, childhood hypotonia, distinctive facial features and sensorineural hearing loss (Kleefstra et al., 2005). Heterozygous mutation in euchromatic histone-lysine N-methyltransferase 1 (EHMT1) is causative for Kleefstra syndrome (Kleefstra et al., 2005). EHMT1 is part of the E2F6 complex which represses transcription via methylation of histone H3K9 (Tachibana et al., 2005).

Heterozygous mutation in lysine (K)-specific methyltransferase 2D (KMT2D, also known as MLL2/MLL4) is associated with Kabuki syndrome (Ng et al., 2010). Kabuki syndrome is characterized by distinctive facial features, mild to moderate mental impairment, microcephaly, hypertonia, skeletal abnormalities, heart abnormalities, and hearing loss (Ng et al., 2010). Hearing loss is a common finding in Kabuki syndrome and can be conductive, sensorineural or mixed (Barozzi et al., 2009). KMT2D is a histone methyltransferase that has been shown to mono- and dimethylate H3K4 (Lee et al., 2013). Approximately 6 percent of Kabuki syndrome cases are caused by heterozygous mutation in lysine (K)-specific demethylase 6A (KDM6A). KDM6A catalyzes the demethylation of H3K27me3. Although, KMT2D and KDM6A have different functions at different lysine residues, both ultimately act as transcriptional activators (Jiang et al., 2013; Lee et al., 2013).

Heterozygous mutations in lysine acetyltransferase 6B (KAT6B, also known as MYST4) are associated with both genitopatellar syndrome (GPS) and Say-Barber-Biesecker variant of Ohdo syndrome (Campeau et al., 2012a; Simpson et al., 2012). These KAT6B-related disorders have phenotypic overlap that includes significant global developmental delay/intellectual disability, hypotonia, cryptorchidism, patellar hypoplasia/agenesis, congenital heart defects, dental anomalies, hearing loss, and thyroid anomalies (Campeau et al., 2012a; Simpson et al., 2012). KAT6B is a HAT that also has transcriptional activation activity in the N-terminal end of the protein and transcriptional repression activity in the C-terminal end of the protein (Campeau et al., 2012b). Mutations leading to GPS occur in the proximal portion of the last exon and lead to the expression of a protein without a C-terminal domain, while mutations leading to Say-Barber-Biesecker variant of Ohdo syndrome occur either throughout the gene, leading to nonsense-mediated decay, or more distally in the last exon (Campeau et al., 2012b).

CHD7 has been more extensively characterized in regards to inner ear development than the epigenetic factors listed above. CHD7 haploinsufficiency causes CHARGE syndrome, the most consistent clinical feature associated with CHARGE is inner ear defects, including semicircular canal dysplasia that typically affects all three canals, and a Mondini form of cochlear hypoplasia (Layman et al., 2010; Zentner et al., 2010). Inner ear phenotypes observed in mouse models of CHARGE syndrome are similar to those reported in CHARGE patients and include semicircular canal defects, innervation defects, and vestibular dysfunction (Kiernan et al., 2002; Hawker et al., 2005; Adams et al., 2007; Hurd et al., 2007). Complete absence of CHD7 results in decreased expression of patterning and pro-neural genes including Otx2, Fgf10, Ngn1, NeuroD, Islet1, Rarb, and Rxrg genes leading to reduced proliferation of developing neuroblasts and inner ear malformations (Hurd et al., 2010; Micucci et al., 2014). Additionally, a recent report found that CHD7 deficiency triggers both p53 expression and activation (Van Nostrand et al., 2014). These data together provide a basis for understanding how CHD7 deficiency results in the profound yet variable phenotypes associated with CHARGE syndrome and potential targets for therapeutic development.

Epigenetics: Damage and Protection

Auditory hair cells have repeatedly been shown to be susceptible to ototoxicity from a multitude of drugs including aminoglycoside antibiotics such as gentamicin, loop diuretics such as furosemide, platinum-based chemotherapy agents such as cisplatin, and a number of non-steroidal anti-inflammatory drugs (NSAIDS). Additionally, noise is one of the most common causes of hearing loss, and one of the most common occupational illnesses in the United States. The formation of reactive oxygen species (ROS) is the major cause that underlies the molecular pathology of hair cell death related to noise induced trauma as well as aminoglycoside antibiotic and cisplatin treatment (Cheng et al., 2005; Schacht et al., 2012). ROS production is associated with increased DNA damage and chromosomal degradation with alterations of both hypermethylation and hypomethylation of the DNA (Campos et al., 2007; Lim et al., 2008; Donkena et al., 2010; Ziech et al., 2010, 2011). Aminoglycoside antibiotics have also been shown to cause increased histone deacetylation in mammalian hair cells through recruitment of HDACs to the chromatin (Jiang et al., 2006; Chen et al., 2009).

HDAC inhibitors were originally used as anti-cancer agents and some are approved by the FDA for use in the treatment of specific types of cancer in humans. However, broad spectrum and HDAC-specific inhibitors are also known to have protective effects in a concentration dependent manner in inflammation, neurodegeneration, and oxidative stress models (Ryu et al., 2003; Liu et al., 2012a; Robert and Rassool, 2012). HDAC inhibitors are primarily thought to modulate chromatin condensation by regulating histone acetylation and thus affect gene expression. HDAC inhibitors have also been shown to affect the post-translational modification of some important intracellular non-histone proteins, such as heat shock protein 90 and Rel-A/p65 (Yu et al., 2002; Faraco et al., 2009). In the inner ear, HDAC inhibitors also have a protective effect on hair cells subjected to aminoglycosides in vitro (Chen et al., 2009) and cisplatin in vivo (Drottar et al., 2006). However, the precise mechanism underlying their protective effect in the inner ear remains unknown.

Cellular Reprogramming and Hair Cell Regeneration

Reprogramming cell fate through transcription factor(s) over-expression is a general and powerful approach for regenerative medicine (Cohen and Melton, 2011). However, direct reprogramming through ectopic expression of defined transcription factors is a slow and inefficient process that requires weeks, with most cells failing to reprogram (Huangfu et al., 2008; Mikkelsen et al., 2008). Additionally, the efficiency and yield of cellular reprogramming rapidly declines with increasing age and differentiation status of the donor cell (Hanna et al., 2010; Kim et al., 2010; Lister et al., 2011). A large reconfiguration of the chromatin structure, from DNA methylation to histone modifications and nucleosome remodeling, occurs during somatic cell reprogramming to a pluripotent state. These layers of epigenetic regulation are often used as repressive mechanisms in somatic cells to prevent unwanted gene expression from other lineages. How these epigenetic barriers to reprogramming are overcome is a key question, since the epigenetic memory of the somatic cell largely impacts its capacity for cellular reprogramming. Several lines of evidence support the notion that the process of reprogramming involves rare stochastic epigenetic events. Studies have shown that inhibitors of epigenetic events such as DNA methylation, histone deacetylation, and histone methylation are able to improve reprogramming efficiency (Huangfu et al., 2008; Mikkelsen et al., 2008; Hanna et al., 2010; Kim et al., 2010; Lister et al., 2011).

The epigenetic modifications made during inner ear development remain mostly unknown at this time. However, cofactors of repressive complexes such as NuRD and PRC2 have been reported to be present in the neonatal mouse organ of Corti. The NuRD cofactors including LSD1 are present throughout most of the organ of Corti from E18.5 until P4, then completely absent by P7, and are detectable again from P8 through P21 (Layman et al., 2013). The PRC2 enzymatic subunit, EZH2 is also highly present from E18.5 to P0 in the mouse organ of Corti, absent between P2 and P4, and is evident again throughout the organ of Corti by P6 and persists through P21 (Layman et al., 2013). The presence of these repressive complexes also correlates with transcriptional silencing of known target genes of LSD1 and EZH2 including genes required for proliferation (mTert) and cell fate specification (Atoh1), which is consistent with reports related to organ of Corti quiescence and maturation during neonatal development. Additionally, DNMT3A and DNMT3B are also reported to have a dramatic increase in expression after the first postnatal week in the mouse organ of Corti (Mutai et al., 2009; Layman et al., 2013). DNA methylation is one of the most common and irreversible epigenetic modifications that control gene expression. DNA methylation may repress genes encoding drug metabolizing enzymes, drug transporters, or even drug target genes, which may alter the pharmacokinetics and pharmacodynamics of drugs that may be ototoxic or drugs designed to facilitate hearing regeneration. A better understanding of how the genes in the inner ear are regulated epigenetically will allow researchers the ability to design therapeutic agents that may bypass or alter the chromatin state making it more amenable to cellular reprogramming.

Unlike mammalian hair cells, hair cells in the avian basilar papilla and utricle are rapidly regenerated after ototoxic injury (Corwin and Oberholtzer, 1997). New avian hair cells are generated from the epithelial supporting cells through renewed supporting cell proliferation and by direct cellular conversion from a supporting cell to a hair cell (Raphael, 1992; Weisleder and Rubel, 1993; Roberson et al., 2004). However, pharmacological inhibition of HDACs results in decreased proliferation of avian vestibular supporting cells, both in dissociated culture and in intact utricles (Slattery et al., 2009). The reduction in supporting cell proliferation causes a reduction in the number of regenerated hair cells but does not directly affect hair cell differentiation (Slattery et al., 2009). These data indicate that HDACs have a critical function in regulating gene expression in non-sensory epithelial cells responding to ototoxic insult during normal regenerative processes. Further analysis is needed to determine which genes are being regulated by HDACs and whether each specific gene is critical for the proliferative response in supporting cells. Naturally regenerating systems such as the avian basilar papilla and utricle provide much needed information about the regulatory networks that are required for hair cell regeneration. Ideally, a comparison of the DNA methylome and histone modifications between the naturally regenerating system and the mammalian system following ototoxic insult would provide vital information about the types of epigenetic events that must occur to achieve complete mammalian hair cell regeneration.

The cell type specific distribution of histone modifications, DNA methylation, and chromatin remodeling events needs to be characterized during inner ear development from a multipotent progenitor cell to a terminally differentiated cell. The mammalian inner ear offers unique challenges for evaluating the epigenome given the limited number of cells across a diversity of cell types. Transcriptomic analysis of the mammalian inner ear during development by microarray or RNA-seq may provide a starting point for analyzing epigenetic modifications that may regulate the expression of specific target genes and microRNAs (Elkan-Miller et al., 2011; Smeti et al., 2012; Rudnicki et al., 2014). Emerging evidence has shown that more than one hundred microRNAs are regulated by epigenetic mechanisms, and about 50% of them are modulated by DNA methylation (Saito et al., 2006; Kunej et al., 2011; Suzuki et al., 2011). Additionally, a subgroup of microRNAs has been shown to directly target the enzymatic effectors of epigenetic modifications, which adds more complexity to the epigenetic regulatory network (Garzon et al., 2009; Iorio et al., 2010).

Conclusions

Given that epigenetics is a cornerstone of development and cellular reprogramming, it seems likely that understanding and manipulating the epigenome holds enormous promise for preventing and treating hearing loss in humans. Epigenetics also offers an important window to understanding how ototoxic compounds and noise affect gene regulatory networks and how these epigenetic modifications may be manipulated and overcome utilizing epigenetic therapeutics. Understanding epigenetic mechanisms has become a major focus for research in most biological systems. The field of hearing research could greatly benefit from the vast amounts of information that can be garnered from epigenetic work in other biological systems to gain a better understanding of the complex gene regulatory networks being regulated in the inner ear.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

References

Adams, M. E., Hurd, E. A., Beyer, L. A., Swiderski, D. L., Raphael, Y., and Martin, D. M. (2007). Defects in vestibular sensory epithelia and innervation in mice with loss of Chd7 function: implications for human CHARGE syndrome. J. Comp. Neurol. 504, 519–532. doi: 10.1002/cne.21460

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Azuara, V., Perry, P., Sauer, S., Spivakov, M., Jorgensen, H. F., John, R. M., et al. (2006). Chromatin signatures of pluripotent cell lines. Nat. Cell Biol. 8, 532–538. doi: 10.1038/ncb1403

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Bannister, A. J., and Kouzarides, T. (2011). Regulation of chromatin by histone modifications. Cell Res. 21, 381–395. doi: 10.1038/cr.2011.22

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Barozzi, S., Di Berardino, F., Atzeri, F., Filipponi, E., Cerutti, M., Selicorni, A., et al. (2009). Audiological and vestibular findings in the Kabuki syndrome. Am. J. Med. Genet. A 149A, 171–176. doi: 10.1002/ajmg.a.32610

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Bernstein, B. E., Mikkelsen, T. S., Xie, X., Kamal, M., Huebert, D. J., Cuff, J., et al. (2006). A bivalent chromatin structure marks key developmental genes in embryonic stem cells. Cell 125, 315–326. doi: 10.1016/j.cell.2006.02.041

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Bosch, A., and Suau, P. (1995). Changes in core histone variant composition in differentiating neurons: the roles of differential turnover and synthesis rates. Eur. J. Cell Biol. 68, 220–225.

Pubmed Abstract | Pubmed Full Text | Google Scholar

Bossuyt, W., Kazanjian, A., De Geest, N., Van Kelst, S., De Hertogh, G., Geboes, K., et al. (2009). Atonal homolog 1 is a tumor suppressor gene. PLoS Biol. 7:e39. doi: 10.3410/f.1157437.617596

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Brandt, W. F., Strickland, W. N., Strickland, M., Carlisle, L., Woods, D., and Von Holt, C. (1979). A histone programme during the life cycle of the sea urchin. Eur. J. Biochem. 94, 1–10. doi: 10.1111/j.1432-1033.1979.tb12864.x

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Byvoet, P., Shepherd, G. R., Hardin, J. M., and Noland, B. J. (1972). The distribution and turnover of labeled methyl groups in histone fractions of cultured mammalian cells. Arch. Biochem. Biophys. 148, 558–567. doi: 10.1016/0003-9861(72)90174-9

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Calvanese, V., Fernandez, A. F., Urdinguio, R. G., Suarez-Alvarez, B., Mangas, C., Perez-Garcia, V., et al. (2012). A promoter DNA demethylation landscape of human hematopoietic differentiation. Nucleic Acids Res. 40, 116–131. doi: 10.1093/nar/gkr685

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Campeau, P. M., Kim, J. C., Lu, J. T., Schwartzentruber, J. A., Abdul-Rahman, O. A., Schlaubitz, S., et al. (2012a). Mutations in KAT6B, encoding a histone acetyltransferase, cause Genitopatellar syndrome. Am. J. Hum. Genet. 90, 282–289. doi: 10.1016/j.ajhg.2011.11.023

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Campeau, P. M., Lu, J. T., Dawson, B. C., Fokkema, I. F., Robertson, S. P., Gibbs, R. A., et al. (2012b). The KAT6B-related disorders genitopatellar syndrome and Ohdo/SBBYS syndrome have distinct clinical features reflecting distinct molecular mechanisms. Hum. Mutat. 33, 1520–1525. doi: 10.1002/humu.22141

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Campos, A. C., Molognoni, F., Melo, F. H., Galdieri, L. C., Carneiro, C. R., D’almeida, V., et al. (2007). Oxidative stress modulates DNA methylation during melanocyte anchorage blockade associated with malignant transformation. Neoplasia 9, 1111–1121. doi: 10.1593/neo.07712

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Campos, E. I., and Reinberg, D. (2009). Histones: annotating chromatin. Annu. Rev. Genet. 43, 559–599. doi: 10.1146/annurev.genet.032608.103928

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Chen, F. Q., Schacht, J., and Sha, S. H. (2009). Aminoglycoside-induced histone deacetylation and hair cell death in the mouse cochlea. J. Neurochem. 108, 1226–1236. doi: 10.1111/j.1471-4159.2009.05871.x

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Cheng, A. G., Cunningham, L. L., and Rubel, E. W. (2005). Mechanisms of hair cell death and protection. Curr. Opin. Otolaryngol. Head Neck. Surg. 13, 343–348. doi: 10.1097/01.moo.0000186799.45377.63

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Cohen, D. E., and Melton, D. (2011). Turning straw into gold: directing cell fate for regenerative medicine. Nat. Rev. Genet. 12, 243–252. doi: 10.1038/nrg2938

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Colbert, L. E., Petrova, A. V., Fisher, S. B., Pantazides, B. G., Madden, M. Z., Hardy, C. W., et al. (2014). CHD7 expression predicts survival outcomes in patients with resected pancreatic cancer. Cancer Res. 74, 2677–2687. doi: 10.1158/0008-5472.can-13-1996

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Corwin, J. T., and Oberholtzer, J. C. (1997). Fish n’ chicks: model recipes for hair-cell regeneration? Neuron 19, 951–954. doi: 10.1016/S0896-6273(00)80386-4

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Deaton, A. M., and Bird, A. (2011). CpG islands and the regulation of transcription. Genes Dev. 25, 1010–1022. doi: 10.1101/gad.2037511

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Donkena, K. V., Young, C. Y., and Tindall, D. J. (2010). Oxidative stress and DNA methylation in prostate cancer. Obstet. Gynecol. Int. 2010:302051. doi: 10.1155/2010/302051

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Drottar, M., Liberman, M. C., Ratan, R. R., and Roberson, D. W. (2006). The histone deacetylase inhibitor sodium butyrate protects against cisplatin-induced hearing loss in guinea pigs. Laryngoscope 116, 292–296. doi: 10.1097/01.mlg.0000197630.85208.36

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Elkan-Miller, T., Ulitsky, I., Hertzano, R., Rudnicki, A., Dror, A. A., Lenz, D. R., et al. (2011). Integration of transcriptomics, proteomics and microRNA analyses reveals novel microRNA regulation of targets in the mammalian inner ear. PLoS One 6:e18195. doi: 10.1371/journal.pone.0018195

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Faraco, G., Pittelli, M., Cavone, L., Fossati, S., Porcu, M., Mascagni, P., et al. (2009). Histone deacetylase (HDAC) inhibitors reduce the glial inflammatory response in vitro and in vivo. Neurobiol. Dis. 36, 269–279. doi: 10.1016/j.nbd.2009.07.019

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Feng, S., Jacobsen, S. E., and Reik, W. (2010). Epigenetic reprogramming in plant and animal development. Science 330, 622–627. doi: 10.1126/science.1190614

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Fuchs, G., Hollander, D., Voichek, Y., Ast, G., and Oren, M. (2014). Cotranscriptional histone H2B monoubiquitylation is tightly coupled with RNA polymerase II elongation rate. Genome Res. 24, 1572–1583. doi: 10.1101/gr.176487.114

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Garzon, R., Liu, S., Fabbri, M., Liu, Z., Heaphy, C. E., Callegari, E., et al. (2009). MicroRNA-29b induces global DNA hypomethylation and tumor suppressor gene reexpression in acute myeloid leukemia by targeting directly DNMT3A and 3B and indirectly DNMT1. Blood 113, 6411–6418. doi: 10.1182/blood-2008-07-170589

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Grove, G. W., and Zweidler, A. (1984). Regulation of nucleosomal core histone variant levels in differentiating murine erythroleukemia cells. Biochemistry 23, 4436–4443. doi: 10.1021/bi00314a030

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Gubbels, S. P., Woessner, D. W., Mitchell, J. C., Ricci, A. J., and Brigande, J. V. (2008). Functional auditory hair cells produced in the mammalian cochlea by in utero gene transfer. Nature 455, 537–541. doi: 10.1038/nature07265

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Hanna, J. H., Saha, K., and Jaenisch, R. (2010). Pluripotency and cellular reprogramming: facts, hypotheses, unresolved issues. Cell 143, 508–525. doi: 10.1016/j.cell.2010.10.008

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Hawker, K., Fuchs, H., Angelis, M. H., and Steel, K. P. (2005). Two new mouse mutants with vestibular defects that map to the highly mutable locus on chromosome 4. Int. J. Audiol. 44, 171–177. doi: 10.1080/14992020500057434

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

He, Y. F., Li, B. Z., Li, Z., Liu, P., Wang, Y., Tang, Q., et al. (2011). Tet-mediated formation of 5-carboxylcytosine and its excision by TDG in mammalian DNA. Science 333, 1303–1307. doi: 10.1126/science.1210944

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Helin, K., and Dhanak, D. (2013). Chromatin proteins and modifications as drug targets. Nature 502, 480–488. doi: 10.1038/nature12751

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Hojo, K., Imamura, T., Takanashi, M., Ishii, K., Sasaki, M., Imura, S., et al. (1999). Hereditary sensory neuropathy with deafness and dementia: a clinical and neuroimaging study. Eur. J. Neurol. 6, 357–361. doi: 10.1046/j.1468-1331.1999.630357.x

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Holliday, R., and Pugh, J. E. (1975). DNA modification mechanisms and gene activity during development. Science 187, 226–232. doi: 10.1126/science.1111098

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Hon, G. C., Rajagopal, N., Shen, Y., Mccleary, D. F., Yue, F., Dang, M. D., et al. (2013). Epigenetic memory at embryonic enhancers identified in DNA methylation maps from adult mouse tissues. Nat. Genet. 45, 1198–1206. doi: 10.1038/ng.2746

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Huang, J., Sengupta, R., Espejo, A. B., Lee, M. G., Dorsey, J. A., Richter, M., et al. (2007). p53 is regulated by the lysine demethylase LSD1. Nature 449, 105–108. doi: 10.1038/nature06092

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Huangfu, D., Maehr, R., Guo, W., Eijkelenboom, A., Snitow, M., Chen, A. E., et al. (2008). Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds. Nat. Biotechnol. 26, 795–797. doi: 10.1038/nbt1418

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Hurd, E. A., Capers, P. L., Blauwkamp, M. N., Adams, M. E., Raphael, Y., Poucher, H. K., et al. (2007). Loss of Chd7 function in gene-trapped reporter mice is embryonic lethal and associated with severe defects in multiple developing tissues. Mamm. Genome 18, 94–104. doi: 10.1007/s00335-006-0107-6

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Hurd, E. A., Poucher, H. K., Cheng, K., Raphael, Y., and Martin, D. M. (2010). The ATP-dependent chromatin remodeling enzyme CHD7 regulates pro-neural gene expression and neurogenesis in the inner ear. Development 137, 3139–3150. doi: 10.1242/dev.047894

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Iorio, M. V., Piovan, C., and Croce, C. M. (2010). Interplay between microRNAs and the epigenetic machinery: an intricate network. Biochim. Biophys. Acta 1799, 694–701. doi: 10.1016/j.bbagrm.2010.05.005

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Ito, S., Shen, L., Dai, Q., Wu, S. C., Collins, L. B., Swenberg, J. A., et al. (2011). Tet proteins can convert 5-methylcytosine to 5-formylcytosine and 5-carboxylcytosine. Science 333, 1300–1333. doi: 10.1126/science.1210597

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Izumikawa, M., Minoda, R., Kawamoto, K., Abrashkin, K. A., Swiderski, D. L., Dolan, D. F., et al. (2005). Auditory hair cell replacement and hearing improvement by Atoh1 gene therapy in deaf mammals. Nat. Med. 11, 271–276. doi: 10.1038/nm1193

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Jackson, M., Krassowska, A., Gilbert, N., Chevassut, T., Forrester, L., Ansell, J., et al. (2004). Severe global DNA hypomethylation blocks differentiation and induces histone hyperacetylation in embryonic stem cells. Mol. Cell. Biol. 24, 8862–8871. doi: 10.1128/mcb.24.20.8862-8871.2004

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Jiang, H., Sha, S. H., and Schacht, J. (2006). Kanamycin alters cytoplasmic and nuclear phosphoinositide signaling in the organ of Corti in vivo. J. Neurochem. 99, 269–276. doi: 10.1111/j.1471-4159.2006.04117.x

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Jiang, W., Wang, J., and Zhang, Y. (2013). Histone H3K27me3 demethylases KDM6A and KDM6B modulate definitive endoderm differentiation from human ESCs by regulating WNT signaling pathway. Cell Res. 23, 122–130. doi: 10.1038/cr.2012.119

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Jurkowska, R. Z., Jurkowski, T. P., and Jeltsch, A. (2011). Structure and function of mammalian DNA methyltransferases. Chembiochem 12, 206–222. doi: 10.1002/cbic.201000195

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Kelly, M. C., Chang, Q., Pan, A., Lin, X., and Chen, P. (2012). Atoh1 directs the formation of sensory mosaics and induces cell proliferation in the postnatal mammalian cochlea in vivo. J. Neurosci. 32, 6699–6710. doi: 10.1523/jneurosci.5420-11.2012

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Kiernan, A. E., Erven, A., Voegeling, S., Peters, J., Nolan, P., Hunter, J., et al. (2002). ENU mutagenesis reveals a highly mutable locus on mouse Chromosome 4 that affects ear morphogenesis. Mamm. Genome 13, 142–148. doi: 10.1007/bf02684018

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Kim, M. S., Chung, N. G., Kang, M. R., Yoo, N. J., and Lee, S. H. (2011). Genetic and expressional alterations of CHD genes in gastric and colorectal cancers. Histopathology 58, 660–668. doi: 10.1111/j.1365-2559.2011.03819.x

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Kim, K., Doi, A., Wen, B., Ng, K., Zhao, R., Cahan, P., et al. (2010). Epigenetic memory in induced pluripotent stem cells. Nature 467, 285–290. doi: 10.1038/nature09342

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Kleefstra, T., Smidt, M., Banning, M. J., Oudakker, A. R., Van Esch, H., De Brouwer, A. P., et al. (2005). Disruption of the gene Euchromatin Histone Methyl Transferase1 (Eu-HMTase1) is associated with the 9q34 subtelomeric deletion syndrome. J. Med. Genet. 42, 299–306. doi: 10.1136/jmg.2004.028464

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Klein, C. J., Botuyan, M. V., Wu, Y., Ward, C. J., Nicholson, G. A., Hammans, S., et al. (2011). Mutations in DNMT1 cause hereditary sensory neuropathy with dementia and hearing loss. Nat. Genet. 43, 595–600. doi: 10.1038/ng.830

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Kolasinska-Zwierz, P., Down, T., Latorre, I., Liu, T., Liu, X. S., and Ahringer, J. (2009). Differential chromatin marking of introns and expressed exons by H3K36me3. Nat. Genet. 41, 376–381. doi: 10.1038/ng.322

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Kontaki, H., and Talianidis, I. (2010). Lysine methylation regulates E2F1-induced cell death. Mol. Cell 39, 152–160. doi: 10.1016/j.molcel.2010.06.006

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Kooistra, S. M., and Helin, K. (2012). Molecular mechanisms and potential functions of histone demethylases. Nat. Rev. Mol. Cell Biol. 13, 297–311. doi: 10.1038/nrm3327

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Kornberg, R. D. (1974). Chromatin structure: a repeating unit of histones and DNA. Science 184, 868–871. doi: 10.1126/science.184.4139.868

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Kornberg, R. D., and Thomas, J. O. (1974). Chromatin structure; oligomers of the histones. Science 184, 865–868. doi: 10.1126/science.184.4139.865

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Kriaucionis, S., and Heintz, N. (2009). The nuclear DNA base 5-hydroxymethylcytosine is present in Purkinje neurons and the brain. Science 324, 929–930. doi: 10.1126/science.1169786

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Kunej, T., Godnic, I., Ferdin, J., Horvat, S., Dovc, P., and Calin, G. A. (2011). Epigenetic regulation of microRNAs in cancer: an integrated review of literature. Mutat. Res. 717, 77–84. doi: 10.1016/j.mrfmmm.2011.03.008

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Layman, W. S., Hurd, E. A., and Martin, D. M. (2010). Chromodomain proteins in development: lessons from CHARGE syndrome. Clin. Genet. 78, 11–20. doi: 10.1111/j.1399-0004.2010.01446.x

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Layman, W. S., Sauceda, M. A., and Zuo, J. (2013). Epigenetic alterations by NuRD and PRC2 in the neonatal mouse cochlea. Hear. Res. 304, 167–178. doi: 10.1016/j.heares.2013.07.017

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Lee, J. E., Wang, C., Xu, S., Cho, Y. W., Wang, L., Feng, X., et al. (2013). H3K4 mono- and di-methyltransferase MLL4 is required for enhancer activation during cell differentiation. Elife 2:e01503. doi: 10.3410/f.718218082.793490513

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Li, E., Bestor, T. H., and Jaenisch, R. (1992). Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell 69, 915–926. doi: 10.1016/0092-8674(92)90611-f

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Li, H., Collado, M., Villasante, A., Strati, K., Ortega, S., Canamero, M., et al. (2009). The Ink4/Arf locus is a barrier for iPS cell reprogramming. Nature 460, 1136–1139. doi: 10.1038/nature08290

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Lim, S. O., Gu, J. M., Kim, M. S., Kim, H. S., Park, Y. N., Park, C. K., et al. (2008). Epigenetic changes induced by reactive oxygen species in hepatocellular carcinoma: methylation of the E-cadherin promoter. Gastroenterology 135, 2128–2140, 2140.e1–2148.e2. doi: 10.1053/j.gastro.2008.07.027

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Lister, R., Pelizzola, M., Dowen, R. H., Hawkins, R. D., Hon, G., Tonti-Filippini, J., et al. (2009). Human DNA methylomes at base resolution show widespread epigenomic differences. Nature 462, 315–322. doi: 10.1038/nature08514

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Lister, R., Pelizzola, M., Kida, Y. S., Hawkins, R. D., Nery, J. R., Hon, G., et al. (2011). Hotspots of aberrant epigenomic reprogramming in human induced pluripotent stem cells. Nature 471, 68–73. doi: 10.1038/nature09798

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Liu, X. S., Chopp, M., Kassis, H., Jia, L. F., Hozeska-Solgot, A., Zhang, R. L., et al. (2012a). Valproic acid increases white matter repair and neurogenesis after stroke. Neuroscience 220, 313–321. doi: 10.1016/j.neuroscience.2012.06.012

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Liu, Z., Dearman, J. A., Cox, B. C., Walters, B. J., Zhang, L., Ayrault, O., et al. (2012b). Age-dependent in vivo conversion of mouse cochlear pillar and Deiters’ cells to immature hair cells by Atoh1 ectopic expression. J. Neurosci. 32, 6600–6610. doi: 10.1523/JNEUROSCI.0818-12.2012

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Luger, K., Mader, A. W., Richmond, R. K., Sargent, D. F., and Richmond, T. J. (1997). Crystal structure of the nucleosome core particle at 2.8 A resolution. Nature 389, 251–260. doi: 10.1038/38444

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Maze, I., Noh, K. M., Soshnev, A. A., and Allis, C. D. (2014). Every amino acid matters: essential contributions of histone variants to mammalian development and disease. Nat. Rev. Genet. 15, 259–271. doi: 10.1038/nrg3673

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Melberg, A., Hetta, J., Dahl, N., Nennesmo, I., Bengtsson, M., Wibom, R., et al. (1995). Autosomal dominant cerebellar ataxia deafness and narcolepsy. J. Neurol. Sci. 134, 119–129. doi: 10.1016/0022-510X(95)00228-0

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Micucci, J. A., Layman, W. S., Hurd, E. A., Sperry, E. D., Frank, S. F., Durham, M. A., et al. (2014). CHD7 and retinoic acid signaling cooperate to regulate neural stem cell and inner ear development in mouse models of CHARGE syndrome. Hum. Mol. Genet. 23, 434–448. doi: 10.1093/hmg/ddt435

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Mikkelsen, T. S., Hanna, J., Zhang, X., Ku, M., Wernig, M., Schorderet, P., et al. (2008). Dissecting direct reprogramming through integrative genomic analysis. Nature 454, 49–55. doi: 10.1038/nature07056

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Mikkelsen, T. S., Ku, M., Jaffe, D. B., Issac, B., Lieberman, E., Giannoukos, G., et al. (2007). Genome-wide maps of chromatin state in pluripotent and lineage-committed cells. Nature 448, 553–560. doi: 10.1038/nature06008

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Mosammaparast, N., Kim, H., Laurent, B., Zhao, Y., Lim, H. J., Majid, M. C., et al. (2013). The histone demethylase LSD1/KDM1A promotes the DNA damage response. J. Cell. Biol. 203, 457–470. doi: 10.1084/jem.21012oia55

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Mutai, H., Nagashima, R., Sugitani, Y., Noda, T., Fujii, M., and Matsunaga, T. (2009). Expression of Pou3f3/Brn-1 and its genomic methylation in developing auditory epithelium. Dev. Neurobiol. 69, 913–930. doi: 10.1002/dneu.20746

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Ng, S. B., Bigham, A. W., Buckingham, K. J., Hannibal, M. C., Mcmillin, M. J., Gildersleeve, H. I., et al. (2010). Exome sequencing identifies MLL2 mutations as a cause of Kabuki syndrome. Nat. Genet. 42, 790–793. doi: 10.1038/ng.646

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Okano, M., Bell, D. W., Haber, D. A., and Li, E. (1999). DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell 99, 247–257. doi: 10.1016/s0092-8674(00)81656-6

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Onder, T. T., Kara, N., Cherry, A., Sinha, A. U., Zhu, N., Bernt, K. M., et al. (2012). Chromatin-modifying enzymes as modulators of reprogramming. Nature 483, 598–602. doi: 10.1038/nature10953

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Pina, B., and Suau, P. (1987). Changes in histones H2A and H3 variant composition in differentiating and mature rat brain cortical neurons. Dev. Biol. 123, 51–58. doi: 10.1016/0012-1606(87)90426-x

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Pleasance, E. D., Stephens, P. J., O’meara, S., Mcbride, D. J., Meynert, A., Jones, D., et al. (2010). A small-cell lung cancer genome with complex signatures of tobacco exposure. Nature 463, 184–190. doi: 10.1038/nature08629

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Qiao, Q., Li, Y., Chen, Z., Wang, M., Reinberg, D., and Xu, R. M. (2011). The structure of NSD1 reveals an autoregulatory mechanism underlying histone H3K36 methylation. J. Biol. Chem. 286, 8361–8368. doi: 10.1074/jbc.m110.204115

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Raphael, Y. (1992). Evidence for supporting cell mitosis in response to acoustic trauma in the avian inner ear. J. Neurocytol. 21, 663–671. doi: 10.1007/bf01191727

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Riggs, A. D. (1975). X inactivation, differentiation and DNA methylation. Cytogenet. Cell Genet. 14, 9–25. doi: 10.1159/000130315

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Roberson, D. W., Alosi, J. A., and Cotanche, D. A. (2004). Direct transdifferentiation gives rise to the earliest new hair cells in regenerating avian auditory epithelium. J. Neurosci. Res. 78, 461–471. doi: 10.1002/jnr.20271

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Robert, C., and Rassool, F. V. (2012). HDAC inhibitors: roles of DNA damage and repair. Adv. Cancer Res. 116, 87–129. doi: 10.1016/B978-0-12-394387-3.00003-3

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Robertson, K. D., Ait-Si-Ali, S., Yokochi, T., Wade, P. A., Jones, P. L., and Wolffe, A. P. (2000). DNMT1 forms a complex with Rb, E2F1 and HDAC1 and represses transcription from E2F-responsive promoters. Nat. Genet. 25, 338–342. doi: 10.1038/77124

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Rudnicki, A., Isakov, O., Ushakov, K., Shivatzki, S., Weiss, I., Friedman, L. M., et al. (2014). Next-generation sequencing of small RNAs from inner ear sensory epithelium identifies microRNAs and defines regulatory pathways. BMC Genomics 15:484. doi: 10.1186/1471-2164-15-484

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Rugg-Gunn, P. J., Cox, B. J., Ralston, A., and Rossant, J. (2010). Distinct histone modifications in stem cell lines and tissue lineages from the early mouse embryo. Proc. Natl. Acad. Sci. U S A 107, 10783–10790. doi: 10.1073/pnas.0914507107

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Ryu, H., Lee, J., Olofsson, B. A., Mwidau, A., Dedeoglu, A., Escudero, M., et al. (2003). Histone deacetylase inhibitors prevent oxidative neuronal death independent of expanded polyglutamine repeats via an Sp1-dependent pathway. Proc. Natl. Acad. Sci. U S A 100, 4281–4286. doi: 10.1073/pnas.0737363100

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Saito, Y., Liang, G., Egger, G., Friedman, J. M., Chuang, J. C., Coetzee, G. A., et al. (2006). Specific activation of microRNA-127 with downregulation of the proto-oncogene BCL6 by chromatin-modifying drugs in human cancer cells. Cancer Cell 9, 435–443. doi: 10.1016/j.ccr.2006.04.020

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Schacht, J., Talaska, A. E., and Rybak, L. P. (2012). Cisplatin and aminoglycoside antibiotics: hearing loss and its prevention. Anat. Rec. (Hoboken) 295, 1837–1850. doi: 10.1002/ar.22578

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Shen, L., and Zhang, Y. (2013). 5-Hydroxymethylcytosine: generation, fate and genomic distribution. Curr. Opin. Cell Biol. 25, 289–296. doi: 10.1016/j.ceb.2013.02.017

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Simpson, M. A., Deshpande, C., Dafou, D., Vissers, L. E., Woollard, W. J., Holder, S. E., et al. (2012). De novo mutations of the gene encoding the histone acetyltransferase KAT6B cause Genitopatellar syndrome. Am. J. Hum. Genet. 90, 290–294. doi: 10.1016/j.ajhg.2011.11.024

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Slattery, E. L., Speck, J. D., and Warchol, M. E. (2009). Epigenetic influences on sensory regeneration: histone deacetylases regulate supporting cell proliferation in the avian utricle. J. Assoc. Res. Otolaryngol. 10, 341–353. doi: 10.1007/s10162-009-0166-y

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Smeti, I., Assou, S., Savary, E., Masmoudi, S., and Zine, A. (2012). Transcriptomic analysis of the developing and adult mouse cochlear sensory epithelia. PLoS One 7:e42987. doi: 10.1371/journal.pone.0042987

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Smith, Z. D., and Meissner, A. (2013). DNA methylation: roles in mammalian development. Nat. Rev. Genet. 14, 204–220. doi: 10.1038/nrg3354

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Stadler, M. B., Murr, R., Burger, L., Ivanek, R., Lienert, F., Scholer, A., et al. (2011). DNA-binding factors shape the mouse methylome at distal regulatory regions. Nature 480, 490–495. doi: 10.1038/nature10716

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Stroud, H., Feng, S., Morey Kinney, S., Pradhan, S., and Jacobsen, S. E. (2011). 5-Hydroxymethylcytosine is associated with enhancers and gene bodies in human embryonic stem cells. Genome Biol. 12:R54. doi: 10.1186/gb-2011-12-6-r54

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Sun, Z., Wu, Y., Ordog, T., Baheti, S., Nie, J., Duan, X., et al. (2014). Aberrant signature methylome by DNMT1 hot spot mutation in hereditary sensory and autonomic neuropathy 1E. Epigenetics 9, 1184–1193. doi: 10.4161/epi.29676

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Suzuki, H., Takatsuka, S., Akashi, H., Yamamoto, E., Nojima, M., Maruyama, R., et al. (2011). Genome-wide profiling of chromatin signatures reveals epigenetic regulation of MicroRNA genes in colorectal cancer. Cancer Res. 71, 5646–5658. doi: 10.1158/0008-5472.can-11-1076

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Szulwach, K. E., Li, X., Li, Y., Song, C. X., Han, J. W., Kim, S., et al. (2011). Integrating 5-hydroxymethylcytosine into the epigenomic landscape of human embryonic stem cells. PLoS Genet. 7:e1002154. doi: 10.1371/journal.pgen.1002154

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Tachibana, M., Ueda, J., Fukuda, M., Takeda, N., Ohta, T., Iwanari, H., et al. (2005). Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3–K9. Genes Dev. 19, 815–826. doi: 10.1101/gad.1284005

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Tahara, T., Yamamoto, E., Madireddi, P., Suzuki, H., Maruyama, R., Chung, W., et al. (2014). Colorectal carcinomas with CpG island methylator phenotype 1 frequently contain mutations in chromatin regulators. Gastroenterology 146, 530–538.e5. doi: 10.1053/j.gastro.2013.10.060

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Tahiliani, M., Koh, K. P., Shen, Y., Pastor, W. A., Bandukwala, H., Brudno, Y., et al. (2009). Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in mammalian DNA by MLL partner TET1. Science 324, 930–935. doi: 10.1126/science.1170116

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Tatton-Brown, K., Murray, A., Hanks, S., Douglas, J., Armstrong, R., Banka, S., et al. (2013). Weaver syndrome and EZH2 mutations: clarifying the clinical phenotype. Am. J. Med. Genet. A 161A, 2972–2980. doi: 10.1002/ajmg.a.36229

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Tatton-Brown, K., and Rahman, N. (2013). The NSD1 and EZH2 overgrowth genes, similarities and differences. Am. J. Med. Genet. C Semin. Med. Genet. 163C, 86–91. doi: 10.1002/ajmg.c.31359

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Tsumura, A., Hayakawa, T., Kumaki, Y., Takebayashi, S., Sakaue, M., Matsuoka, C., et al. (2006). Maintenance of self-renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b. Genes Cells 11, 805–814. doi: 10.1111/j.1365-2443.2006.00984.x

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Valinluck, V., Tsai, H. H., Rogstad, D. K., Burdzy, A., Bird, A., and Sowers, L. C. (2004). Oxidative damage to methyl-CpG sequences inhibits the binding of the methyl-CpG binding domain (MBD) of methyl-CpG binding protein 2 (MeCP2). Nucleic Acids Res. 32, 4100–4108. doi: 10.1093/nar/gkh739

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Van Nostrand, J. L., Brady, C. A., Jung, H., Fuentes, D. R., Kozak, M. M., Johnson, T. M., et al. (2014). Inappropriate p53 activation during development induces features of CHARGE syndrome. Nature 514, 228–232. doi: 10.1038/nature13585

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Vaquero, A., Loyola, A., and Reinberg, D. (2003). The constantly changing face of chromatin. Sci. Aging Knowledge Environ. 2003:RE4. doi: 10.1126/sageke.2003.14.re4

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Velasco, G., Hube, F., Rollin, J., Neuillet, D., Philippe, C., Bouzinba-Segard, H., et al. (2010). Dnmt3b recruitment through E2F6 transcriptional repressor mediates germ-line gene silencing in murine somatic tissues. Proc. Natl. Acad. Sci. U S A 107, 9281–9286. doi: 10.1073/pnas.1000473107

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Voigt, P., Tee, W. W., and Reinberg, D. (2013). A double take on bivalent promoters. Genes Dev. 27, 1318–1338. doi: 10.1101/gad.219626.113

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Waddington, C. H. (2012). The epigenotype. 1942. Int. J. Epidemiol. 41, 10–13. doi: 10.1093/ije/dyr184

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Wang, J., Hevi, S., Kurash, J. K., Lei, H., Gay, F., Bajko, J., et al. (2009). The lysine demethylase LSD1 (KDM1) is required for maintenance of global DNA methylation. Nat. Genet. 41, 125–129. doi: 10.1038/ng.268

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Wang, T., Pan, Q., Lin, L., Szulwach, K. E., Song, C. X., He, C., et al. (2012). Genome-wide DNA hydroxymethylation changes are associated with neurodevelopmental genes in the developing human cerebellum. Hum. Mol. Genet. 21, 5500–5510. doi: 10.1093/hmg/dds394

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Weisleder, P., and Rubel, E. W. (1993). Hair cell regeneration after streptomycin toxicity in the avian vestibular epithelium. J. Comp. Neurol. 331, 97–110. doi: 10.1002/cne.903310106

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Winkelmann, J., Lin, L., Schormair, B., Kornum, B. R., Faraco, J., Plazzi, G., et al. (2012). Mutations in DNMT1 cause autosomal dominant cerebellar ataxia, deafness and narcolepsy. Hum. Mol. Genet. 21, 2205–2210. doi: 10.1093/hmg/dds035

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Wright, A., and Dyck, P. J. (1995). Hereditary sensory neuropathy with sensorineural deafness and early-onset dementia. Neurology 45, 560–562. doi: 10.1212/wnl.45.3.560

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Wu, S. C., and Zhang, Y. (2009). Minireview: role of protein methylation and demethylation in nuclear hormone signaling. Mol. Endocrinol. 23, 1323–1334. doi: 10.1210/me.2009-0131

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Wunsch, A. M., Reinhardt, K., and Lough, J. (1991). Normal transitions in synthesis of replacement histones H2A.Z and H3.3 during differentiation of dystrophic myotube cells. A brief note. Mech. Ageing Dev. 59, 299–305. doi: 10.1016/0047-6374(91)90140-u

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Yildirim, O., Li, R., Hung, J. H., Chen, P. B., Dong, X., Ee, L. S., et al. (2011). Mbd3/NURD complex regulates expression of 5-hydroxymethylcytosine marked genes in embryonic stem cells. Cell 147, 1498–1510. doi: 10.1016/j.cell.2011.11.054

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Yu, X., Guo, Z. S., Marcu, M. G., Neckers, L., Nguyen, D. M., Chen, G. A., et al. (2002). Modulation of p53, ErbB1, ErbB2 and Raf-1 expression in lung cancer cells by depsipeptide FR901228. J. Natl. Cancer Inst. 94, 504–513. doi: 10.1093/jnci/94.7.504

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Yu, M., Hon, G. C., Szulwach, K. E., Song, C. X., Zhang, L., Kim, A., et al. (2012). Base-resolution analysis of 5-hydroxymethylcytosine in the mammalian genome. Cell 149, 1368–1380. doi: 10.1016/j.cell.2012.04.027

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Zeng, L., and Zhou, M. M. (2002). Bromodomain: an acetyl-lysine binding domain. FEBS Lett. 513, 124–128. doi: 10.1016/s0014-5793(01)03309-9

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Zentner, G. E., Layman, W. S., Martin, D. M., and Scacheri, P. C. (2010). Molecular and phenotypic aspects of CHD7 mutation in CHARGE syndrome. Am. J. Med. Genet. A 152A, 674–686. doi: 10.1002/ajmg.a.33323

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Zhang, K., and Dent, S. Y. (2005). Histone modifying enzymes and cancer: going beyond histones. J. Cell. Biochem. 96, 1137–1148. doi: 10.1002/jcb.20615

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Zheng, J. L., and Gao, W. Q. (2000). Overexpression of Math1 induces robust production of extra hair cells in postnatal rat inner ears. Nat. Neurosci. 3, 580–586. doi: 10.1038/75753

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Ziech, D., Franco, R., Pappa, A., Malamou-Mitsi, V., Georgakila, S., Georgakilas, A. G., et al. (2010). The role of epigenetics in environmental and occupational carcinogenesis. Chem. Biol. Interact. 188, 340–349. doi: 10.1016/j.cbi.2010.06.012

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Ziech, D., Franco, R., Pappa, A., and Panayiotidis, M. I. (2011). Reactive oxygen species (ROS)–induced genetic and epigenetic alterations in human carcinogenesis. Mutat. Res. 711, 167–173. doi: 10.1016/j.mrfmmm.2011.02.015

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar

Ziller, M. J., Gu, H., Muller, F., Donaghey, J., Tsai, L. T., Kohlbacher, O., et al. (2013). Charting a dynamic DNA methylation landscape of the human genome. Nature 500, 477–481. doi: 10.1038/nature12433

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text | Google Scholar