ORIGINAL RESEARCH article
Front. Cell. Infect. Microbiol.
Sec. Clinical Microbiology
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1558612
Methodology and Application of multiplex PCR-dipstick DNA Chromatography for eight Respiratory Bacterial Pathogens Detection
Provisionally accepted
- 1People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China
- 2Guangzhou Baochuang Biotechnology Co., Ltd., Guangzhou, China
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The multiplex PCR-dipstick DNA chromatography assay demonstrated remarkable efficacy, being capable of specifically discriminating among the eight pathogens within a remarkably short timeframe of 40 minutes. The detection limit for individual bacterial species ranged from 10 to 10² CFU/mL. Notably, no cross-reactions were observed among the eight target bacteria, nor with other representative respiratory bacteria, including A. junii, E. cloacae, K. oxytoca, H. parainfluenzae, P. fluorescens, A. hydrophila, and S. epidermidis. The concordance between the results obtained from the multiplex PCR-dipstick DNA chromatography assay and those from DNA sequencing was absolute, with a kappa value of 1.00.A successful multiplex PCR-dipstick DNA chromatography assay was established for the simultaneous detection of eight respiratory bacterial pathogens and was effectively applied in clinical sample analysis. This indicates that this single-use device has promising potential for analyzing the microbial composition related to respiratory infections and is also suitable for small laboratories and field diagnostics.
Keywords: multiplex PCR-dipstick DNA chromatography assay, Lower respiratory tract infection, eight respiratory bacterial pathogens, Rapid detection, Multiple detection
Received: 10 Jan 2025; Accepted: 17 Apr 2025.
Copyright: © 2025 Hu, Wang and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Xiuri Wang, People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China
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