Massa Medicata Fermentata treated spleen deficiency constipation by mediating intestinal microbiota and serum peptide

Kangxiao GUO ^1* Yuan Tang ¹ Yongwang Yan ^1,2 Tao Yang ²

• ¹ Changsha Health Vocational College, Changsha, China
• ² Central South University Forestry and Technology, Changsha, Hunan Province, China

Objectives:To investigate the correlation between the treatment of spleen deficiency constipation and the typical brain and intestinal peptides, Methods: 18 male Kunming mice were randomly divided into three treatment groups (n=6): Normal groups (CC), Model groups (CM), and Massa Medicata Fermentata intervention group(CG). CM and CG were established a spleen deficiency constipation mouse model. After the mold is finished, the CG was infused with 0.15 g/mL Massa Medicata Fermentata water infusion at a dose of 4 g/(kg day), twice a day at 0.4 mL. An equal amount of distilled water was infused in CC and CM for 7 days. The body weight and faecal water content of the mice were monitored during the modeling. Following the intervention, 16S rRNA amplicon sequencing was used to analyze changes in the microflora in the intestinal contents, and serum SP, VIP, and CGRP levels were determined by ELISA. Results: The modeling had no significant effect on the weight of the mice, the water content of the mice's faeces was greatly reduced, and the faeces was dry and hard. Constipation caused by spleen deficiency can lead to a decrease in serum SP and an increase in VIP and GCRP. After treatment with Massa Medicata Fermentata, SP, VIP and GCRP all have a correction. Intestinal microbiota diversity of mice with spleen deficiency constipation, and the dominant microbiota and characteristic microbiota changed, indicating that the intestinal microbiota was unbalanced. After the intervention of Massa Medicata Fermentata, the intestinal microbiota diversity of spleen deficiency constipation mice increased. Through the analysis of characteristic microbiota and serum SP, VIP, and GCRP levels, Ligilactobacillus was found to be positively correlated with SP and negatively correlated with VIP, while Akkermansia and Streptococcus were negatively correlated with SP, Candidates Arthromitus was negatively correlated with GCRP, Akkermansia, Candidatus Arthromitus were negatively correlated with VIP, and Candidates arthromitus was negatively correlated with GCRP. Conclusions:Massa Medicata Fermentata can affect the secretion of short-chain fatty acids in the intestine by altering the micro-ecological environment of the intestine, and then affect the secretion of serum peptides in mice and alleviate the spleen deficiency constipation .