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BRIEF RESEARCH REPORT article
Front. Cell. Infect. Microbiol.
Sec. Molecular Bacterial Pathogenesis
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1549523
The metagenomic and whole-genome metagenomic detection of multidrug-resistant bacteria from subclinical mastitis-affected cow's milk in India
Provisionally accepted
Ayyasamy Manimaran 1*
Arumugam Desingu 2*
Arumugam Kumaresan 1 Poonam Singh 1
Komala Subramanya 1 Pramod Dodamani 1 Parmar Ankitkumar Dineshbhai 1- 1 National Dairy Research Institute (Southern Regional Station), Bengaluru, India
- 2 Institute of Advanced Virology (IAV), Thiruvananthapuram, Kerala, India
Multidrug-resistant (MDR) bacteria in farm animal products threaten human health by causing zoonotic infections. The complete genome sequences of MDR bacteria from the subclinical mastitis-affected cows’ milk have not yet been comprehensively documented in India. In this study, using a bacterial metagenomic sequencing approach, we detected the nearly complete genome sequences of Pseudomonas veronii and Staphylococcus xylosus from the subclinical mastitis-affected cow’s milk. Furthermore, we sequenced the nearly complete genome sequences of Escherichia coli, Klebsiella pneumoniae, Staphylococcus hominis, and Staphylococcus xylosus from the subclinical mastitis-affected cow’s milk using a whole-genome metagenomic sequencing method. Our analysis subsequently revealed that the complete genome sequences of the identified bacteria contained MDR genes and genes for multiple virulence factors. These MDR bacteria may pose a public health risk through exposure to milkers, milk handlers, and farm workers or through the handling and consumption of unpasteurized milk.
Keywords: ESKAPE pathogens, pathogenic bacteria, Multidrug-resistant bacteria, mastitisaffected cow's milk, E coli
Received: 31 Dec 2024; Accepted: 21 Mar 2025.
Copyright: © 2025 Manimaran, Desingu, Kumaresan, Singh, Subramanya, Dodamani and Dineshbhai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Ayyasamy Manimaran, National Dairy Research Institute (Southern Regional Station), Bengaluru, India
Arumugam Desingu, Institute of Advanced Virology (IAV), Thiruvananthapuram, 695 317, Kerala, India
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