ORIGINAL RESEARCH article
Front. Cell. Infect. Microbiol.
Sec. Bacteria and Host
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1524820
This article is part of the Research TopicBacteria-Host Interactions: From Infection to CarcinogenesisView all 12 articles
In vitro impact of Streptococcus mitis on the inhibition of oral cancer cell proliferation via mitotic modulation
Provisionally accepted
Inori Inui1
Shinichi Mochizuki2Fumika Hirabayashi-Nishimuta1Yoshie Yoshioka1Osamu Takahashi1Masaaki Sasaguri1Manabu Habu1
Wataru Ariyoshi1
Ryota Yamasaki1,3*- 1Kyushu Dental University, Kokurakita-ku, Kitakyūshū, Japan
- 2University of Kitakyushu, Kitakyushu, Fukuoka, Japan
- 3Kyushu Institute of Technology, Kitakyushu, Fukuoka, Japan
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Recent studies have elucidated a potential correlation between oral carcinogenesis and the oral microbiome. However, few reports exist on the interaction between Streptococcus spp., the most common oral microflora bacterium, and oral cancer. In this study, we aimed to elucidate the effects of Streptococcus spp. on oral squamous cell carcinoma (OSCC) cells in vitro. Methods: HSC-3 (tongue carcinoma) and Ca9-22 (gingival carcinoma) cells were used as models of OSCC cells, and their responses were examined after adding major oral Streptococcus species-S. mitis, S. sanguinis, S. anginosus, S. salivarius, and S. mutans-to the culture medium. Cell viability was assessed using the CCK-8 assay. Gene expression changes were analyzed using RNA sequencing and RT-qPCR followed by Gene Ontology analysis. Flow cytometry was used to observe the effects of bacteria on the cell cycle. Results: Among all examined Streptococcus species, S. mitis had the strongest inhibitory effect on the growth of OSCC cells. RNA sequencing and RT-qPCR revealed an increase in the number of genes involved in mitotic nuclear division, especially DUSP1, in HSC-3 cells treated with S. mitis. Flow cytometry showed that S. mitis caused a decreased number of HSC-3 cells in the G0/G1 phase and an increased number in the G2/M phase, suggesting cell cycle arrest in the G2/M phase. Various treatments of S. mitis were used to examine the effects of intact bacteria and bacterial components on cancer cells, indicating the involvement of structural bacterial proteins. Conclusions: This study, investigating the association between oral cancer cells and bacteria of the genus Streptococcus, revealed that S. mitis may play an important role in the inhibition of cancer cells.
Keywords: Oral microbiota, Streptococcus spp., oral cancer, Carcinoma, Cell Cycle, DUSP1, mitotic nuclear division, Streptococcus mitis
Received: 08 Nov 2024; Accepted: 17 Apr 2025.
Copyright: © 2025 Inui, Mochizuki, Hirabayashi-Nishimuta, Yoshioka, Takahashi, Sasaguri, Habu, Ariyoshi and Yamasaki. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Ryota Yamasaki, Kyushu Dental University, Kokurakita-ku, Kitakyūshū, Japan
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