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ORIGINAL RESEARCH article
Front. Cell. Infect. Microbiol.
Sec. Intestinal Microbiome
Volume 15 - 2025 |
doi: 10.3389/fcimb.2025.1514201
Chaihuang Qingyi Huoxue Granule Ameliorates Severe Acute Pancreatitis by Modulating Gut Microbiota and Repairing the Intestinal Mucosal Barrier
Provisionally accepted
XiaoBin Zhang 1
Xusen Zeng 2*
Wen Guo 1
Xin Zhou 2 Yi Zhang 3*
Mingyun Tang 1 Juan Fu 2*
Yuqing Deng 1 Xin Liang 1* Long Zhao 2* Zhi Li 2* Tiangang Wang 2* Li Li 2*
Guohui Xiao 2*- 1 College of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, Sichuan Province, China
- 2 Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, China
- 3 Department of Spleen and Gastroenterology, Leshan Traditional Chinese Medicine Hospital, Leshan, China
Background: During severe acute pancreatitis (SAP), damage to the intestinal mucosal barrier and translocation of intestinal pathogenic bacteria are key mechanisms that accelerate the disease progression of SAP. Chaihuang Qingyi Huoxue Granule (CH) is a herbal formula used in the clinical treatment of SAP. This study aims to investigate the role of CH in regulating gut microbiota and intestinal mucosal barrier in SAP rats.Methods: Sodium taurocholate (3.5%) was retrogradely perfused into the biliopancreatic duct to establish the model of SAP in rats. CH (4.4 g/kg) was administered by gavage. Serum amylase, lipase, and endotoxin levels were measured. Hematoxylin-eosin (HE) staining was used to observe morphological changes in the pancreas and colon. The expression of zona occludens-1 (ZO-1) and occludin in the colon was examined by immunohistochemistry (IHC) and western blot. 16S rDNA gene sequencing was used to analyze the gut microbiota of the rats. The content of short-chain fatty acids (SCFAs) in the intestinal contents of the rats was determined by gas chromatography-mass spectrometry (GC-MS). Results: CH reduced serum amylase, lipase, and endotoxin levels in SAP rats, alleviated pathological damage in the pancreas and colon, and restored the expression of ZO-1 and occludin. Moreover, CH alleviated gut microbiota dysbiosis in SAP rats, with restored gut microbiota diversity and structure. At the phylum level, the relative abundance of Firmicutes and Bacteroidetes increased, while that of Proteobacteria decreased. At the genus level, the abundance of Ruminococcus 1, Parabacteroides, Prevotellaceae UCG-001, Lachnospiraceae NK4A136 group, and Lactobacillus increased, while that of Escherichia-Shigella, Enterococcus, and Enterobacter decreased. In addition, CH increased the levels of SCFAs in the intestinal contents of SAP rats.Conclusion: CH ameliorates SAP by maintaining the homeostasis and diversity of the gut microbiota, increasing the levels of SCFAs, and repairing the intestinal mucosal barrier.
Keywords: Severe acute pancreatitis, Traditional Chinese Medicine, Chaihuang Qingyi Huoxue Granule, Gut Microbiota, Intestinal Mucosal Barrier, short-chain fatty acids
Received: 20 Oct 2024; Accepted: 24 Jan 2025.
Copyright: © 2025 Zhang, Zeng, Guo, Zhou, Zhang, Tang, Fu, Deng, Liang, Zhao, Li, Wang, Li and Xiao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Xusen Zeng, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, China
Yi Zhang, Department of Spleen and Gastroenterology, Leshan Traditional Chinese Medicine Hospital, Leshan, China
Juan Fu, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, China
Xin Liang, College of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, 646000, Sichuan Province, China
Long Zhao, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, China
Zhi Li, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, China
Tiangang Wang, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, China
Li Li, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, China
Guohui Xiao, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, China
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