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ORIGINAL RESEARCH article
Front. Cell. Infect. Microbiol.
Sec. Fungal Pathogenesis
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1502579
This article is part of the Research Topic Applications of Fungi View all 5 articles
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Introduction: Over the past three decades, dengue disease incidence has significantly increased worldwide, creating serious public health concerns. The principal mosquito vector, Aedes aegypti, exhibits resistance to commonly used insecticides, reducing the efficacy of vector control measures. Thus, the necessity for alternate strategies is critical. Using bioinsecticides such as entomopathogenic fungi (EPF) is one such strategy. This study details the evaluation of mycoparasitic Trichoderma atroviride and entomopathogenic Aspergillus niger against pyrethroid resistant and susceptible Ae. aegypti, populations. Materials and methods: Molecular identification of the isolated entomopathogenic fungal strains was done using ITS-rDNA sequence data. Larvicidal and adulticidal assays were performed using different spore concentrations of fungal species. Pupal emergence was assessed from the survived larvae of larvicidal assays. Results: Larvicidal assays revealed the highest mortality of 60% for T. atroviride after 9 days of exposure when compared with the highest mortality of 52% of A. niger after 6 days of exposure. No significant difference was observed between the pyrethroid-resistant and susceptible mosquito colonies, suggesting a lack of connection between prior resistance status and EPF pathogenicity. No pupal mortality was observed, although pupal duration was prolonged. Both EPF strains exhibited 100% mortality in adulticidal assays, signifying the potential use of the two fungal species as adulticides. Conclusion: However, further studies are needed to understand the biology of EPF, mechanism of action, mosquito immune pathways activated, as well as the effect on non-target organisms. The findings have implications for the possible use of A. niger and T. atroviride as potential bioinsecticides against the control of Ae. aegypti.
Keywords: bioinsecticides, entomopathogenic fungi, Mosquito Control, Dengue, Aedes aegypti
Received: 27 Sep 2024; Accepted: 17 Mar 2025.
Copyright: © 2025 Banduwardena, Mendis, Thambugala, Fernando and Promputtha. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
H S D Fernando, University of Sri Jayewardenepura, Nugegoda, Sri Lanka
I Promputtha, Chiang Mai University, Chiang Mai, 50200, Thailand
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
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