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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Virus and Host
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1499658

Inflammatory and Neuropathological Responses to Vesiculovirus Carajas Encephalitis in Adult Mice: Variability, Tolerance and Resistance

Provisionally accepted
Maria Sueli Barbosa Cavalcante Maria Sueli Barbosa Cavalcante 1Diego Siqueira Santos Diego Siqueira Santos 2Lidineuza Machado Araujo Lidineuza Machado Araujo 2Priscilla dos Santos Lieuthier Freitas Priscilla dos Santos Lieuthier Freitas 2Carlos Augusto Moreira Silva Carlos Augusto Moreira Silva 3Karina Glazziane Barbosa Carvalho Karina Glazziane Barbosa Carvalho 2Marinalva Tereza Fereira de Araujo Marinalva Tereza Fereira de Araujo 2Eliana Vieira Pinto da Silva Eliana Vieira Pinto da Silva 4Ana Paula Drummond Rodrigues Farias Ana Paula Drummond Rodrigues Farias 2Daniel Guerreiro Diniz Daniel Guerreiro Diniz 5Cristovam Wanderley Picanço Diniz Cristovam Wanderley Picanço Diniz 5Jose Antonio Picanço Diniz Jose Antonio Picanço Diniz 2*
  • 1 Programa de Pós-Graduação em Neurociências e Biologia Celular, Universidade Federal do Pará, Belem, Brazil
  • 2 Instituto Evandro Chagas, Belem, Brazil
  • 3 Instituto de Patologia Cirúrgica e Molecular, Belém, Pará, Brasil, Belem, Brazil
  • 4 Evandro Chagas Institute, Ananindeua, Brazil
  • 5 Universidade Federal do Pará, Hospital Universitário João de Barros Barreto, Laboratório de Investigações em Neurodegeneração e Infecção, Belém, Pará, Brasil., Belem, Brazil

The final, formatted version of the article will be published soon.

    This study examines the neuroinflammatory response and variability in sickness behavior caused by Vesiculovirus carajas (CARV) encephalitis in adult BALB/c mice. By administering infected and uninfected brain homogenates intranasally, we observed a range of neuropathological features, cytokine changes, and clinical symptoms, all indicative of inflammatory modulation. CARV antigens were primarily detected in necrotic neurons, with microglial activation occurring mainly near the ventricles and blood vessels. By day 10 post-infection, infected mice exhibited significantly elevated levels of MCP-1, IFN-γ, IL-12 p70, TNF-α, IL-6, and IL-10 in the brain. Neuroinvasion was determined to occur via hematogenous pathways, distinguishing CARV from other vesiculovirus species. The variability in these findings may reflect different levels of tolerance and resistance to the infection.

    Keywords: Vesiculovirus carajas, Encephalitis, Host inflammatory response, Cytokines, encephabilitis

    Received: 21 Sep 2024; Accepted: 05 Feb 2025.

    Copyright: © 2025 Cavalcante, Santos, Araujo, Lieuthier Freitas, Silva, Carvalho, de Araujo, Silva, Rodrigues Farias, Guerreiro Diniz, Picanço Diniz and Picanço Diniz. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Jose Antonio Picanço Diniz, Instituto Evandro Chagas, Belem, Brazil

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