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BRIEF RESEARCH REPORT article

Front. Cell. Infect. Microbiol.
Sec. Veterinary and Zoonotic Infection
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1490948

Visible and Rapid Detection of Feline Chaphamaparvovirus Using Multienzyme Isothermal Rapid Amplification and Lateral Flow Dipstick Assay

Provisionally accepted
Jun Ji Jun Ji 1*Xinhao Mu Xinhao Mu 1Shunshun Pan Shunshun Pan 1Xin Xu Xin Xu 1Shiyuan Zhang Shiyuan Zhang 1Honghui Huang Honghui Huang 1Ying Li Ying Li 1Yingzuo Bi Yingzuo Bi 2Lunguang Yao Lunguang Yao 1
  • 1 Nanyang Normal University, Nanyang, China
  • 2 South China Agricultural University, Guangzhou, Guangdong Province, China

The final, formatted version of the article will be published soon.

    Feline chaphamaparvovirus (FeChPV) is a novel parvovirus previously reported in Canadian cats and Chinese dogs with diarrhea in 2019 and 2020, respectively. Herein, we aimed to establish a simple detection method for FeChPV in field clinics. The primers and probes for the multienzyme isothermal rapid amplification and lateral flow dipstick (MIRA-LFD) assay were designed to target the conserved regions of the FeChPV genome and determine the optimal reaction temperature and time. Without relying on precision instruments, FeChPV detection using the MIRA-LFD assay was completed within 20 min at 37°C, without any cross-reaction with other reference viruses. The newly established MIRA-LFD assay had a detection limit of 32.3 copies/L, which was 10-fold lower than that of the nested polymerase chain reaction (PCR) assay. Furthermore, the MIRA-LFD assay detected 29 FeChPV-positive samples among 417 cats with diarrhea, providing a slightly higher positivity rate than the nested PCR assay. These results indicate that the newly developed MIRA-LFD assay for FeChPV detection is an efficient, economical, reliable, and simple method that can help in the early prevention and control of FeChPV infection.

    Keywords: FeChPV, MIRA-LFD, detection, Sensitivity, simplicity

    Received: 12 Sep 2024; Accepted: 02 Jan 2025.

    Copyright: © 2025 Ji, Mu, Pan, Xu, Zhang, Huang, Li, Bi and Yao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Jun Ji, Nanyang Normal University, Nanyang, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.