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ORIGINAL RESEARCH article
Front. Cell. Infect. Microbiol.
Sec. Antibiotic Resistance and New Antimicrobial drugs
Volume 15 - 2025 |
doi: 10.3389/fcimb.2025.1490528
Establishment and validation of a dual qPCR method for the detection of carbapenem-resistant Acinetobacter baumannii in bloodstream infections
Provisionally accepted- 1 Department of Clinical Laboratory, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
- 2 KingMed School of Laboratory Medicine, Guangzhou Medical University, Guangzhou, Guizhou Province, China
- 3 National Clinical Research Center for Respiratory Diseases, Guangzhou, China
Bloodstream infections(BSIs) caused by carbapenem-resistant Acinetobacter baumannii (CRAB) have a high mortality rate due to the high levels of drug resistance. There is an urgent need to establish a sensitive and accurate detection method to rapidly detect CRAB in BSIs.A new method was developed based on fluorescence quantitative PCR (qPCR) targeting the specific region of 16sRNA and OXA-23 gene from CRAB. The parameters were evaluated and optimized. This qPCR method was further applied in the detection of AB from 30 clinical specimens.The qPCR method established in this study showed high specificity. The method successfully differentiated Acinetobacter baumannii(A. baumanii) from 26 other common pathogens in BSIs and identify the carbapenem resistance gene. The qPCR method shows a limit of detection (LOD) of 3×10 -3 ng/μL, and displays good linear relationship between 16sRNA and OXA-23 and excellent repeatability (CV ≤2%). The results for the detection of 30 clinical specimens using this new qPCR method are in complete agreement with those using blood culture and drug susceptibility test.The qPCR method established in this study has strong specificity, wide linear range, good repeatability, and a lower LOD than PCR (Non-fluorescence quantification). The method provides new technical support for the early clinical diagnosis of CRAB in BSIs.
Keywords: bloodstream infections, Carbapenemase resistance, Simultaneously,, crab, qPCR method
Received: 03 Sep 2024; Accepted: 27 Jan 2025.
Copyright: © 2025 Yu, Kou, Liu, Guan and Sun. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Baoqing Sun, Department of Clinical Laboratory, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
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