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ORIGINAL RESEARCH article
Front. Cell. Infect. Microbiol.
Sec. Intestinal Microbiome
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1485048
MAGs-based genomic comparison of gut significantly enriched microbes in obese individuals pre-and post-bariatric surgery across diverse locations
Provisionally accepted- 1 Peking Union Medical College Hospital (CAMS), Beijing, China
- 2 Plastic Surgery Hospital (PSH), Chinese Academy of Medical Sciences andPeking Union Medical College, Beijing, China
AbstractObesity, a global health challenge, is closely linked to distinct gut microbiota profiles. Bariatric surgeries, including Laparoscopic Sleeve Gastrectomy (LSG), Sleeve Gastrectomy (SG), and Roux-en-Y Gastric Bypass (RYGB), not only result in significant weight loss but also induce profound changes in gut microbiota composition and function. This study analyzed four published metagenomic datasets, comprising 500 samples, using a unified bioinformatics workflow. The findings reveal that RYGB most significantly enhances gut microbiota α-diversity among the three procedures. Through cross-cohort integration and three differential analysis methods, 39 species biomarkers were identified, including 27 microbes enriched in the non-obesity group (NonOB_Enrich) and 12 microbes enriched in the obesity group (OB_Enrich). Additionally, 4201 high-quality Metagenome-Assembled Genomes (MAGs) were constructed, consisting of 177 NonOB_Enrich and 14 OB_Enrich MAGs. Comparative genomic analysis revealed that NonOB_Enrich MAGs exhibit distinct carbohydrate degradation and utilization profiles compared to OB_Enrich MAGs. NonOB_Enrich group showed higher proportions of Glycoside Hydrolase Families including GH105, GH2, GH23, GH43, and GlycosylTransferase Family GT0 genes. KEGG analysis showed significant enrichment in amino acid and metabolism pathways. NonOB_Enrich MAGs exhibit distinct gene profiles and higher gene diversity in fatty acid biosynthesis and secondary metabolite genes compared with the OB_Enrich group. Furthermore, based on estimated Peak-to-Trough Ratios (PTRs) of MAGs, Akkermansia muciniphila and Bacteroides uniformis exhibited higher growth rates post-surgery, facilitating the exploration of the mechanisms by which microbial communities contribute to weight loss.Keywords: Obesity, Bariatric surgeries, Gut microbiota, MAGs.
Keywords: Obesity, Bariatric surgeries, Gut Microbiota, MAGS, fatty acid biosynthesis
Received: 23 Aug 2024; Accepted: 27 Feb 2025.
Copyright: © 2025 Shi and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Jia Li, Peking Union Medical College Hospital (CAMS), Beijing, China
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