The final, formatted version of the article will be published soon.
ORIGINAL RESEARCH article
Front. Cell. Infect. Microbiol.
Sec. Clinical Infectious Diseases
Volume 15 - 2025 |
doi: 10.3389/fcimb.2025.1484144
This article is part of the Research Topic Deciphering Brain Complexity through Multi-Omics Research: Insights into Cancers and Non-Neoplastic Diseases View all articles
Cerebrospinal fluid profiles of targeted metabolomics on neurotransmitter in the patients with post-neurosurgical bacterial meningitis
Provisionally accepted- First Affiliated Hospital of Anhui Medical University, Hefei, China
Background: Post-neurosurgical bacterial meningitis (PNBM) is a severe complication of the patients receiving neurosurgical treatments. Pathogen and neuroinflammation have been reported to influence metabolites in microenvironment of central nervous system. However, information about the relationship between neurotransmitter levels and PNBM is still limited. In this study, we aimed to investigate the diagnostic potential of neurotransmitters for PNBM in the patients with stroke. Methods: In this study, a total of 66 stroke patients were recruited. Among that, 40 patients complicated with PNBM. We profiled cerebrospinal fluid (CSF) levels of precursors and metabolites of neurotransmitter with targeted metabolomics method, which contained 26 precursors and metabolites of neurotransmitters, using ultra-performance liquid chromatography coupled with mass spectrometry (UPLC/MS). Results: We found that 14 biomarkers were down-regulated but 3,4-dihydroxyphenylacetic acid (DOPAC) was up-regulated in the CSF of PNBM patients. Among that, D-glutamine (AUC=1.000), Boc-D-Tyr-OH (AUC=0.9447), L(+)-arginine (AUC=0.9418), and DOPAC (AUC=0.9173) had strong diagnostic efficiency for PNBM. Bioinformatic analysis showed that tyrosine metabolism, butanoate metabolism, histidine metabolism, alanine, aspartate and glutamate metabolism, glycerophospholipid metabolism, arginine and proline metabolism, and tryptophan metabolism might be involved in the pathogenesis of PNBM. In reviewing of previous studies, we found a probably diverse pathophysiological alteration between PNBM and community-acquired bacterial meningitis. Conclusions: In summary, we identified the downregulated levels of D-glutamine, Boc-D-Tyr-OH, L(+)-arginine, and phenprobamate, as well as upregulated level of DOPAC in CSF had strong diagnostic efficiencies. The results also offered potential targets in the treatment of PNBM.
Keywords: neurotransmitter, Bacterial meningitis, Neurosurgery, hemorrhagic stroke, tyrosine metabolism Table: 1 table ZX, LG and LY, Software & Formal analysis: LG, Supervision: HC and LY, Funding acquisition: LY and HC
Received: 21 Aug 2024; Accepted: 31 Jan 2025.
Copyright: © 2025 Mei, Guan, Xu, Cheng and Ye. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Hongwei Cheng, First Affiliated Hospital of Anhui Medical University, Hefei, China
Lei Ye, First Affiliated Hospital of Anhui Medical University, Hefei, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.