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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Clinical Infectious Diseases
Volume 14 - 2024 | doi: 10.3389/fcimb.2024.1514046
This article is part of the Research Topic Research Advances toward One Health in Brucellosis View all 6 articles

Preparation and evaluation of Brucella T4SS recombinant proteins in serodiagnosis of human brucellosis based on TMT-based proteomics technology

Provisionally accepted
Qi Wu Qi Wu 1Chen Sun Chen Sun 2Liping Guo Liping Guo 1Yujia Xie Yujia Xie 1Jinpeng Zhang Jinpeng Zhang 1Dehui Yin Dehui Yin 1*
  • 1 Xuzhou Medical University, Xuzhou, China
  • 2 Huai'an Hospital of Huai'an City, Huai'an, China

The final, formatted version of the article will be published soon.

    Brucellosis, a significant zoonotic infectious disease, poses a global health threat.Accurate and efficient diagnosis is crucial for prevention, control, and treatment of brucellosis. VirB proteins, components of the Type IV secretion system (T4SS) in Brucella, play a pivotal role in bacterial virulence and pathogenesis but have been understudied for their diagnostic potential.Tandem Mass Tag (TMT) proteomics technology was utilized to identify highly expressed VirB proteins from wild-type Brucella strains. Recombinant T4SS proteins were prepared, and an indirect ELISA method was established for serological diagnosis of human brucellosis.Seven T4SS proteins (rVirB3, rVirB4, rVirB9, rBMEII0036, rVirB8, rVirB11, and rVirB10) were expressed used to construct the indirect ELISA method which showed high diagnostic accuracy. Sensitivity and specificity of the proteins exceeded 0.9100 and 0.9167, respectively, demonstrating good performance comparable to traditional LPS and Rose Bengal Ag antigens. Cross-reactivity was observed in a limited number of serum samples from febrile patients without brucellosis.The study highlights the potential of VirB proteins as novel diagnostic antigens for human brucellosis. Future research can further optimize the use of VirB proteins in diagnostic assays and explore their applications in vaccine development.

    Keywords: Brucellosis, type IV secretion system, VirB proteins, TMT proteomics, Serodiagnosis

    Received: 19 Oct 2024; Accepted: 27 Dec 2024.

    Copyright: © 2024 Wu, Sun, Guo, Xie, Zhang and Yin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Dehui Yin, Xuzhou Medical University, Xuzhou, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.