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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Intestinal Microbiome
Volume 14 - 2024 | doi: 10.3389/fcimb.2024.1497563
This article is part of the Research Topic The Role of Gut Microbes and Their Metabolites in Immune-related Diseases-Volume II View all 15 articles

Xingnao Jiutan tablets modulate gut microbiota and gut microbiota metabolism to alleviate cerebral ischemia/reperfusion injury

Provisionally accepted
Yanyan Chen Yanyan Chen Jing Zhang Jing Zhang Xiaoran Hou Xiaoran Hou Shijiao Cai Shijiao Cai Jingyue Zhang Jingyue Zhang Yidan Gou Yidan Gou Hanxu Zhang Hanxu Zhang Yang Zhai Yang Zhai HengJie Yuan HengJie Yuan *
  • Department of Pharmacy, General Hospital, Tianjin Medical University, Tianjin, 300052, China, Tianjin, China

The final, formatted version of the article will be published soon.

    Xingnao Jiutan tablets (XNJT), a compound Chinese medicine, have been applied to the treatment of the sequelae of cerebral thrombosis or cerebral hemorrhage, transient cerebral ischemia, and central retinal vein obstruction, etc., but the underlying mechanisms are not yet clear. This research focused on examining the impact of XNJT for cerebral ischemia/reperfusion (MCAO/R) injury, utilizing gut microbiota and metabolomic studies. The primary components of XNJT were identified through the application of the HPLC technique. We established a MCAO/R model in mice and conducted behavioral evaluations, cerebral blood flow measurements, and TTC staining. We used ELISA, high-throughput 16S rDNA gene sequencing, and metabolomics techniques to detect inflammatory factors, microbial populations, and metabolites, respectively. Finally, we performed Spearman correlation analysis to investigate the relationships among gut microbiota and metabolites, comprehensively exploring the mechanisms of XNJT to alleviate cerebral ischemia-reperfusion injury. We discovered that XNJT effectively enhanced neurological performance, alleviated cerebral infarction, diminished neuronal cell death, and increased cerebral blood flow. Moreover, XNJT downregulated the secretion of pro-inflammatory cytokines like TNF, IL-6, and IL-1β. Additionally, XNJT improved gut microbiota levels in MCAO/R mice, particularly Bacteroides, Firmicutes, Escherichia-Shigella, and Ligilactobacillus. Furthermore, XNJT primarily modulated differential metabolites in the gut through Glycerophospholipid, Linoleic acid, and Sphingolipid metabolism pathways. Spearman correlation analysis revealed significant associations among intestinal microbiota and various metabolites. In summary, our findings suggest that XNJT can improve cerebral ischemia/reperfusion injury outcomes, reduce inflammatory responses, and regulate gut microbiota and differential metabolites. It's possible that the potential mechanisms are connected to controlling gut microbiota and metabolism.

    Keywords: Xingnao Jiutan tablets, Cerebral ischemia/reperfusion, Gut Microbiota, Metabolism, brain-gut axis

    Received: 17 Sep 2024; Accepted: 27 Dec 2024.

    Copyright: © 2024 Chen, Zhang, Hou, Cai, Zhang, Gou, Zhang, Zhai and Yuan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: HengJie Yuan, Department of Pharmacy, General Hospital, Tianjin Medical University, Tianjin, 300052, China, Tianjin, China

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