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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Virus and Host
Volume 14 - 2024 | doi: 10.3389/fcimb.2024.1497427
This article is part of the Research Topic The Role of DNA Viruses in Human Cancers Volume II View all articles

KIF20A activated by transcription factor GATA2 to promote cell growth in hepatitis B virus-related hepatocellular carcinoma

Provisionally accepted
Juan X Juan X 1Wenhua C Wenhua C 2Yi W Yi W 3Yunpeng Z Yunpeng Z 3Zhiming W Zhiming W 3Yunyan D Yunyan D 3Yaoxuan L Yaoxuan L 4Pinggui C Pinggui C 3Ting L Ting L 2Yifan L Yifan L 2Gaopeng Li Gaopeng Li 1,3*Wenqing Q Wenqing Q 2Jing Chen Jing Chen 2
  • 1 Shanxi Bethune Hospital, Shanxi Medical University, Taiyuan, China
  • 2 Shanxi Provincial Cancer Hospital, Taiyuan, Shanxi Province, China
  • 3 Third Hospital of Shanxi Medical College, Taiyuan, Shanxi Province, China
  • 4 Shanxi Medical University, Taiyuan, Shanxi Province, China

The final, formatted version of the article will be published soon.

    Background: Elevated evidence suggests that KIF20A plays an important role in hepatocellular carcinoma (HCC) progression. Nevertheless, the underlying mechanism by which KIF20A promotes HCC cell growth are not well understood.Using TCGA-LIHC RNAseq and GEO datasets, we assessed the KIF20A expression and patient survival in HCC and hepatitis B virus (HBV)-related HCC. Mutant and CNV analysis were performed to evaluate the genetic alteration of KIF20A in HCC.PPI network and GSEA enrichment was utilized for analyzing the KIF20A-related genes and involved pathways in HCC. To further explore regulatory mechanism in HBVrelated HCC, PROMO prediction and luciferase reporter system was utilized for verifying HBx/GATA2/KIF20A binding sites. CCK-8 and flow cytometry were carried out to determine the regulation of GATA2-KIF20A on HBV-related HCC cell proliferation and apoptosis.Results: KIF20A was significantly upregulated in pan-cancer (including HCC). KIF20A mRNA level was a significant independent predictor of overall survival in HBV-related HCC patients. Genetic alterations analysis revealed the copy number gain and amplification triggered KIF20A upregulation in HCC. In addition, the genes associated with KIF20A expression in HCC was enriched in PLK1 pathway and cell cycle in HCC.HBx might indirectly binds to KIF20A promoter via regulating GATA2. Additionally, transcription factor GATA2 directly binds to the promoter region of KIF20A.Overexpression of GATA2 promotes HepG2.2.15 cell growth and inhibits cell apoptosis via modulating KIF20A.Our findings demonstrated that HBx contributed to cell proliferation by interacting with GATA2 and KIF20A in HBV-related HCC.

    Keywords: Hepatocellular Carcinoma, Hepatitis B virus, Apoptosis, prognosis, HBx protein

    Received: 17 Sep 2024; Accepted: 23 Oct 2024.

    Copyright: © 2024 X, C, W, Z, W, D, L, C, L, L, Li, Q and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Gaopeng Li, Third Hospital of Shanxi Medical College, Taiyuan, Shanxi Province, China

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