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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Clinical Microbiology
Volume 14 - 2024 | doi: 10.3389/fcimb.2024.1477422

ERA-CRISPR/Cas12a-Based, Fast and Specific diagnostic detection for Chlamydia pneumoniae

Provisionally accepted
Yanxia Zhou Yanxia Zhou 1Zijun Yan Zijun Yan 1Shi Zhou Shi Zhou 1Weiwei Li Weiwei Li 2Hongyu Yang Hongyu Yang 1Hongliang Chen Hongliang Chen 3Zhongliang Deng Zhongliang Deng 4Qilin Zeng Qilin Zeng 1Peiyuan Sun Peiyuan Sun 1Yimou Wu Yimou Wu 5*
  • 1 Institution of Pathogenic Biology, Hengyang Medical School, University of South China, Hengyan, China
  • 2 Department of Clinical Laboratory, The Second People's Hospital of Foshan, Foshan, China
  • 3 The First People's Hospital of Chenzhou, Chenzhou, Hunan Province, China
  • 4 Department of Public Health Laboratory Sciences, College of Public Health, Hengyang Medical School, University of South China, Hengyang, China
  • 5 University of South China, Hengyang, China

The final, formatted version of the article will be published soon.

    Chlamydia pneumoniae (C. pneumoniae) is a specialized intracellular parasitic pathogen capable of causing pneumonia, sinusitis, bronchitis, and other respiratory diseases, which pose significant public health challenges. Therefore, rapid, accurate, and sensitive diagnosis is crucial for the prevention and treatment of respiratory diseases caused by C. pneumoniae. In this study, we combined enzymatic recombination amplification (ERA) with the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) 12a system (CRISPR/Cas12a) to develop a dual detection platform termed the Cpn-ERA-CRISPR/Cas12a dual system. This system integrates both the ERA-CRISPR/Cas12a fluorescence system and the ERA-CRISPR/Cas12a lateral flow system. Detection results can be measured using a fluorescence detector or observed with the naked eye on lateral flow strips. The fluorescence system and the lateral flow system detect C. pneumoniae in 30 minutes and 15 minutes, respectively. This dual system exhibits no cross-reactivity with the other seven pathogens, demonstrating high specificity, and achieves a sensitivity of 10 0 copies/µL. Additionally, the Cpn-ERA-CRISPR/Cas12a

    Keywords: Chlamydia pneumoniae, OmpA gene, CRISPR/Cas12a, Era, pathogenic bacteria detection, Trans-cleavage

    Received: 07 Aug 2024; Accepted: 14 Oct 2024.

    Copyright: © 2024 Zhou, Yan, Zhou, Li, Yang, Chen, Deng, Zeng, Sun and Wu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Yimou Wu, University of South China, Hengyang, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.