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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Clinical Infectious Diseases
Volume 14 - 2024 | doi: 10.3389/fcimb.2024.1456119
This article is part of the Research Topic Advances in the Diagnosis and Management of Infectious Diseases View all 5 articles

The value of metagenomic next-generation sequencing with blood samples for the diagnosis of Disseminated Tuberculosis

Provisionally accepted
Jing Ma Jing Ma Yongfang Jiang Yongfang Jiang Yan He Yan He Huaying Zhou Huaying Zhou *
  • Second Xiangya Hospital, Central South University, Changsha, China

The final, formatted version of the article will be published soon.

    Objective: To assess the clinical value of metagenomic next-generation sequencing (mNGS) of blood samples for the identification of Disseminated Tuberculosis (DTB).Methods: A total of 48 individuals suspected of DTB were enrolled. All patients underwent metagenomic next-generation sequencing (mNGS) of peripheral blood and conventional microbiological tests. Patient's characteristics were collected from their medical records.Results: Twenty-eight patients were diagnosed with DTB, whereas twenty patients were confirmed as non-DTB cases. In the DTB groups, nineteen (67.9%) contained TB sequences, with specific reads of TB ranging from 1 to 219. The TB sequence was more detectable by mNGS in patients with male, elevated PCT levels, HIV positive, and decreased CD4 T cell count. The HIVpositive group shows a higher TB mNGS reads (p=0.012), TB mNGS sensitivity (p=0.05). The sensitivity of TB mNGS in blood samples was 80% for HIV-infected patients, and 44.4% for non-HIV-infected individuals (P=0.05). The non-HIV group had a higher prevalence of military tuberculosis (P = 0.018), and extra-pulmonary tuberculosis was more prevalent in the HIV-positive group.Our research shown that mNGS of blood samples has excellent sensitivity for the diagnosis of DTB. The TB sequence was more detectable by mNGS in patients with elevated PCT levels, HIV positive, and decreased CD4 T cell count.

    Keywords: metagenomic next-generation sequencing, Peripheral Blood, Disseminated tuberculosis, clinical diagnosis, Diagnosis performance

    Received: 28 Jun 2024; Accepted: 12 Nov 2024.

    Copyright: © 2024 Ma, Jiang, He and Zhou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Huaying Zhou, Second Xiangya Hospital, Central South University, Changsha, China

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