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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Veterinary and Zoonotic Infection
Volume 14 - 2024 | doi: 10.3389/fcimb.2024.1452158
This article is part of the Research Topic Evolution, Virulence and Pathogenesis of Viruses in Domestic Birds View all articles

Apoptosis, inflammatory and innate immune responses induced by infection with a novel goose astrovirus in goose embryonic kidney cells

Provisionally accepted
Zhanpeng Hou Zhanpeng Hou 1Shaobing Jin Shaobing Jin 1Yu Liang Yu Liang 1Haiyue Wang Haiyue Wang 1Danli Jiang Danli Jiang 1Nan Cao Nan Cao 1Minhua Sun Minhua Sun 2Yunbo Tian Yunbo Tian 1Wenjun Liu Wenjun Liu 1Danning Xu Danning Xu 1Xinliang Fu Xinliang Fu 1*
  • 1 Zhongkai University of Agriculture and Engineering, Guangzhou, China
  • 2 Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong Province, China

The final, formatted version of the article will be published soon.

    Since 2016, a highly lethal visceral gout induced by infection with the novel goose astrovirus (GoAstV) resulted in an ongoing outbreak in goslings in China, with a mortality rate ranging from 10% to 50%, and causing considerable economic losses in the goose industry.However, the pathogenesis of GoAstV and the molecular mechanism by which kidney lesions are induced by GoAstV infection are unclear. In the present study, a GEK cell infection model for GoAstV was established, and the apoptosis, inflammatory and innate immune responses induced by GoAstV were investigated in GEK cells. The results shown that the expression of proapoptotic proteins, including Bax, caspase-3, caspase-9 and cytochrome c, increased in the infection group; however, the expression of the antiapoptotic protein Bcl-2 decreased, indicating that apoptosis was induced by GoAstV infection in GEK cells. Besides, the activation of the RIG-I/MDA5 pathway and the downstream upregulation of proinflammatory cytokines, including the adapter proteins MAVS, IRF7 and NF-κB and the proinflammatory cytokines IL-6, IL-8 and TNF-α, were detected in GEK cells infected with GoAstV. In addition, GoAstV infection induces the activation of the NLPR3 pathway and further stimulates the increased production of IL-1β. In summary, the present study revealed that GoAstV infection could induce apoptosis and the activation of the RIG-I/MDA5 and NLRP3 pathways in GEK cells, as well as the massive release of proinflammatory cytokines. These results are helpful for elucidating the molecular mechanism of pathological lesions in the kidney in gout goslings infected with GoAstV and the interaction between GoAstV and the innate immune system of the host.

    Keywords: GoAstV, Apoptosis, Inflammatory Response, innate immune, NLRP3

    Received: 20 Jun 2024; Accepted: 07 Oct 2024.

    Copyright: © 2024 Hou, Jin, Liang, Wang, Jiang, Cao, Sun, Tian, Liu, Xu and Fu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Xinliang Fu, Zhongkai University of Agriculture and Engineering, Guangzhou, China

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