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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Molecular Bacterial Pathogenesis
Volume 14 - 2024 | doi: 10.3389/fcimb.2024.1449856

Campylobacter coli of porcine origin exhibit an open pan-genome within a single clonal complex: Insights from comparative genomic analysis

Provisionally accepted
Sandeep Ghatak Sandeep Ghatak 1*Arockiasamy Arun Prince Milton Arockiasamy Arun Prince Milton 1*Samir Das Samir Das 1Kasanchi M. Momin Kasanchi M. Momin 1Kandhan Srinivas Kandhan Srinivas 1Daniel A. Pyngrope Daniel A. Pyngrope 1G Bhuvana Priya G Bhuvana Priya 2
  • 1 Division of Animal and Fisheries Science, ICAR Research Complex for NEH Region, Barapani, India
  • 2 College of Agriculture (CAU, Imphal), Kydremkulai, India

The final, formatted version of the article will be published soon.

    Though Campylobacter spp., including C. coli has emerged as important zoonotic foodborne pathogens globally, the understanding of the genomic epidemiology of C. coli of porcine origin is unavailable. Since pigs are important reservoir of C. coli, we analysed the C. coli genomes that were isolated (n=3) from pigs and sequenced (this study) along with all other C. coli genomes for which pig intestines, pig feces, and pigs were mentioned as source in NCBI database till 6th January, 2023. We report the pan-genomic features, muti-locus sequence types, resistome, virulome, mobilome and phylogenomic analysis of these organisms that were obtained from pigs. Our analysis revealed, that besides having an open pan-genome, majority (63%) of the typeable isolates of pig origin C. coli belonged to a single clonal complex ST-828. Resistome of these C. coli was predominated by tetO (53%), blaOXA-193 (49%) and APH (3')-IIIa (21%) genes, while the virulome analysis revealed a core set 37 virulence genes. Analysis of mobile genetic elements in the genomes revealed wide diversity for plasmids, and bacteriophages, while 30 transposons were common to all genomes of C. coli of porcine origin. Phylogenomic analysis showed two discernible clusters comprising of isolates originating from Japan and another set of isolates comprising mostly copies of type strain stored in three different culture collections.

    Keywords: Campylobacter coli, porcine, Comparative genomics, clonal complex, pangenome

    Received: 25 Jun 2024; Accepted: 28 Aug 2024.

    Copyright: © 2024 Ghatak, Milton, Das, Momin, Srinivas, Pyngrope and Priya. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Sandeep Ghatak, Division of Animal and Fisheries Science, ICAR Research Complex for NEH Region, Barapani, India
    Arockiasamy Arun Prince Milton, Division of Animal and Fisheries Science, ICAR Research Complex for NEH Region, Barapani, India

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