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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Clinical Microbiology
Volume 14 - 2024 | doi: 10.3389/fcimb.2024.1442062
This article is part of the Research Topic Targeted metagenomics in pathogen detection View all 11 articles

Evaluation of Phage-Based Decontamination in Respiratory intensive care unit Environments Using ddPCR and 16S rRNA Targeted Sequencing Techniquese

Provisionally accepted
Yinghan Shi Yinghan Shi 1Weihua Zhang Weihua Zhang 1*Lina Li Lina Li 2*Wencai Wu Wencai Wu 3Mengzhe Li Mengzhe Li 4Kun Xiao Kun Xiao 1Kaifei Wang Kaifei Wang 1Zhaojun Sheng Zhaojun Sheng 1*Xie Fei Xie Fei 1Xiuli Wang Xiuli Wang 1*Xin Shi Xin Shi 1*Yigang Tong Yigang Tong 4*Lixin Xie Lixin Xie 1*
  • 1 College of Pulmonary and Critical Care Medicine, Chinese PLA General Hospital, Beijing, China
  • 2 Fifth Medical Center of the PLA General Hospital, Beijing, Beijing Municipality, China
  • 3 Key Laboratory of Biomedical Information Engineering, Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’ an, Shaanxi Province, China
  • 4 College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, Beijing Municipality, China

The final, formatted version of the article will be published soon.

    Keywor ds: bacter iophages, Hospital-acquir ed infections, dr ug-r esistant bacter ial, Tar geted metagenomics in pathogen, 16S r RNA. Abstr act K. pneumoniae is a major contributor to hospital-acquired infections (HAIs), which are predominantly spread through contamination of hospital environments or medical devices. Research indicates that although current disinfection measures are crucial in controlling the spread of these pathogens, their effectiveness is diminishing due to the development of resistance by some pathogens to existing disinfectants. Moreover, frequent use of chemical disinfectants can cause equipment corrosion, pose hazards to the environment and human health, and may even promote the emergence of new resistance in pathogens. Considering these challenges, developing new disinfectants, particularly those that are both environmentally friendly and highly effective against multidrugresistant organisms, has become increasingly important.In this study, we developed a bacteriophage cocktail targeted at two prevalent clinical strains of carbapenem-resistant Klebsiella pneumoniae (CRKP), ST11 KL47 and ST11 KL64, and employed it as an adjunctive disinfectant in the ward environment through ultrasonic nebulization. Singlemolecule digital PCR quantification showed that the phage mixture, dispersed via an ultrasonic nebulizer, significantly reduced the molecular levels of CRKP. Within the first 24 hours posttreatment, the target pathogen population was significantly decreased. Subsequent measurements

    Keywords: Bacteriophages, Hospital-acquired infections, Drug-resistant bacterial, Targeted metagenomics in pathogen, 16S sRNA

    Received: 01 Jun 2024; Accepted: 01 Aug 2024.

    Copyright: © 2024 Shi, Zhang, Li, Wu, Li, Xiao, Wang, Sheng, Fei, Wang, Shi, Tong and Xie. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Weihua Zhang, College of Pulmonary and Critical Care Medicine, Chinese PLA General Hospital, Beijing, China
    Lina Li, Fifth Medical Center of the PLA General Hospital, Beijing, 100049, Beijing Municipality, China
    Zhaojun Sheng, College of Pulmonary and Critical Care Medicine, Chinese PLA General Hospital, Beijing, China
    Xiuli Wang, College of Pulmonary and Critical Care Medicine, Chinese PLA General Hospital, Beijing, China
    Xin Shi, College of Pulmonary and Critical Care Medicine, Chinese PLA General Hospital, Beijing, China
    Yigang Tong, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, Beijing Municipality, China
    Lixin Xie, College of Pulmonary and Critical Care Medicine, Chinese PLA General Hospital, Beijing, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.