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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Extra-intestinal Microbiome
Volume 14 - 2024 | doi: 10.3389/fcimb.2024.1438777
This article is part of the Research Topic Lung Microbiome in Health and Disease View all 5 articles

Characteristics of low respiratory Microbiota in Children's refractory Mycoplasma pneumoniae pneumonia pre and post COVID 19 era

Provisionally accepted
Aizhen Lu Aizhen Lu *Zhimin Xi Zhimin Xi Jinglong Chen Jinglong Chen Libo Wang Libo Wang
  • Children's Hospital, Fudan University, Shanghai, China

The final, formatted version of the article will be published soon.

    Little was known about the characteristics of low respiratory tract (LRT) microbiota of refractory M. pneumoniae pneumonia (RMPP) in children before and after the COVID-19 pandemic.Forty-two children diagnosed with RMPP in 2019(Y2019 group) and 33 children diagnosed with RMPP in 2023(Y2023 group), entered into the study. The characteristics of the clinical findings were examined, and the LRT microbiota was analyzed by metagenomic next generation sequencing. The ratio of consolidate, atelectasis, lung necrosis, and erythema multiforme in Y2023 group was significantly higher than that in Y2019 (P<0.05). Mycoplasmoides pneumoniae was the top species of the LRT microbiota in both groups. The rate of macrolide resistance MP in Y2023 was significantly higher than that in Y2019 (P<0.05), and the mutant site was all 23S rRNA A2063G. There were no significant differences in α-diversity and β-diversity of LRT microbiota between Y2019 and Y2023 group. Trichoderma_citrinoviride, Canine_mastadenovirus_A, Ralstonia_pickettii, Lactococcus_lactis, Pseudomonas_aeruginosa were the biomarkers of LRT microbiota in children with RMPP of Y2023. The abundance of Mycoplasmoides pneumoniae positively correlated with the levels of D-dimer and LDH, negatively correlated with the counts of CD3+ T cells、CD8+ T cells、CD19+ B cells and CD16+CD56+ NK cells. Our study showed that high abundance of MP was correlated with the severity of RMPP and decrease of immune cells.Trichoderma_citrinoviride, Canine_mastadenovirus_A, Ralstonia_pickettii, Lactococcus_lactis, 2 Pseudomonas_aeruginosa were the biomarkers in microbiota of LRT in children with RMPP post COVID-19 era.

    Keywords: Refractory M. pneumoniae pneumonia (RMPP), COVID-19, Children, Low respiratory tract, microbiota

    Received: 27 May 2024; Accepted: 16 Dec 2024.

    Copyright: © 2024 Lu, Xi, Chen and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Aizhen Lu, Children's Hospital, Fudan University, Shanghai, China

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