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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Molecular Bacterial Pathogenesis
Volume 14 - 2024 | doi: 10.3389/fcimb.2024.1427829
This article is part of the Research Topic The Role of Transcriptional Regulation in Mycobacterium Physiology View all 4 articles

MnoSR removal in Mycobacterium smegmatis triggers broad transcriptional response to 1,3-propanediol and glucose as sole carbon sources

Provisionally accepted
Renata Plocinska Renata Plocinska 1Katarzyna Struś Katarzyna Struś 1Małgorzata Korycka-Machała Małgorzata Korycka-Machała 1Przemysław A. Płociński Przemysław A. Płociński 2Magdalena Kuzioła Magdalena Kuzioła 3Anna Żaczek Anna Żaczek 4Marcin Słomka Marcin Słomka 5Jaroslaw Dziadek Jaroslaw Dziadek 1*
  • 1 Institute for Medical Biology, Polish Academy of Sciences, Łódź, Poland
  • 2 Department of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland
  • 3 Other, Lódz, Poland
  • 4 Other, Rzeszów, Poland
  • 5 Biobank Laboratory, Department of Molecular Biophysics, Faculty of Environmental Protection, University of Lodz, Łódź, Łódź, Poland

The final, formatted version of the article will be published soon.

    The two-component signal transduction systems play an essential role in the adaptation of bacteria to changing environmental conditions. One of them is the MnoSR system involved in the regulation of methylotrophic metabolism in M. smegmatis. The ∆mnoS, ∆mnoR, and ∆mnoS/R mutant strains were generated and cultured in the presence of defined carbon sources.Growth curve analysis confirmed that inactivation of the MnoSR impairs the ability of M. smegmatis cells to use alcohols such as 1,3-propanediol and ethanol but improves the bacterial growth on ethylene glycol, xylitol and glycerol. The total RNA sequencing method was employed to understand the importance of MnoSR in the global responses of mycobacteria to limited carbon access and in carbon-rich conditions. The loss of MnoSR had significant effect on carbon utilization in the case of mycobacteria cultured on glucose or 1,3-propanediol as sole carbon sources as it influenced the expression of multiple metabolic pathways. The numerous transcriptional changes could not be linked to the presence of evident MnoR DNA-binding sites within the promotor regions for the genes outside of the mno operon. This was confirmed by EMSA and microscale thermophoresis with mutated MnoR binding consensus region. Our comprehensive analysis highlights the system's vital role in metabolic adaptability, providing insights into its potential impact on environmental survival of mycobacteria.

    Keywords: histidine sensor kinase MnoS, response regulator MnoR, Mycobacterium smegmatis, Methylotrophic metabolism, RNA-Seq

    Received: 04 May 2024; Accepted: 02 Jul 2024.

    Copyright: © 2024 Plocinska, Struś, Korycka-Machała, Płociński, Kuzioła, Żaczek, Słomka and Dziadek. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Jaroslaw Dziadek, Institute for Medical Biology, Polish Academy of Sciences, Łódź, Poland

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